数字农科院2.0

Differentially expressed genes in the midgut of Silkworm infected with cytoplasmic polyhedrosis virus

文献类型： 外文期刊

作者： Wu, Ping;Li, Mu-Wang;Wang, Xiu-Ye;Liu, Ting;Qin, Guang-Xing;Guo, Xi-Jie;Wang, Xiu;Zhao, Pan;Guo, Xi-Jie

作者机构：

关键词： Bombyx mori;cytoplasmic polyhedrosis virus;midgut;suppression subtractive hybridization;quantitative real-time PCR

期刊名称： AFRICAN JOURNAL OF BIOTECHNOLOGY

ISSN： 1684-5315

年卷期： 2009 年 8 卷 16 期

页码：

收录情况： SCI

摘要： Understanding of the responsive and interactive mechanism between the host cells and Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is crucial to the diagnosis of CPV-caused disease and the development of new control measures. In this report, we employed suppression subtractive hybridization to compare differentially expressed genes in the midguts of CPV-infected and normal silkworm larvae. 36 genes and 20 novel ESTs were obtained from 2 reciprocal subtractive libraries. Three up-regulated genes (ferritin, rpL11 and alkaline nuclease) and 3 down-regulated genes (serine protease, trypsin-like protease and inhibitor of apoptosis protein) were identified by quantitative real-time PCR. The transcript differences of these 6 genes at 6, 12, 24, 48 and 72 h post-inoculation both in CPV-infected and normal midguts were compared. Our results indicated that ferritin and rpL11 were increased during the early stage (6-12h p.i.) of CPV infection, whereas alkaline nuclease was increased during the late stage (24-72h p.i.) of CPV infection. The expression of serine protease and trypsin-like protease is decreased at 24-72 h after CPV infection, while the expression of inhibitor of apoptosis protein is decreased throughout the infective stage. Our results provide new clues for investigating the molecular mechanism of BmCPV infection.

分类号：

  • 相关文献

[1]Cloning and characterization of the gene encoding an ubiquitin-activating enzyme E1 domain-containing protein of silkworm, Bombyx mori. Wu, Ping,Li, Mu-Wang,Guo, Xi-Jie,Jiang, Yun-Feng,Wang, Zi-Sheng,Guo, Xi-Jie. 2010

[2]Novel protein of IBP from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan. 2012

[3]Digital Gene Expression analysis in the midgut of 4008 silkworm strain infected with cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qjan, He-ying,Qin, Guangxing,Guo, Xi-jie,Gao, Kun,Qjan, He-ying,Qin, Guangxing,Guo, Xi-jie. 2014

[4]iTRAQ-based quantitative proteomic analysis of midgut in silkworm infected with Bombyx mori cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-Yuan,Shang, Meng-Ke,Qin, Guang-Xing,Hou, Cheng-Xiang,Guo, Xi-Jie.

[5]Cloning, characterization, and expression analysis of a novel BmGDAP1 gene from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Deng, Xiangyuan.

[6]Gene expression profiling in the thiamethoxam resistant and susceptible B-biotype sweetpotato whitefly, Bemisia tabaci. Zhou, Xu-guo,Xie, Wen,Yang, Xin,Wang, Shao-li,Wu, Qing-jun,Yang, Ni-na,Li, Ru-mei,Jiao, Xiao-guo,Pan, Hui-peng,Liu, Bai-ming,Feng, Yun-tao,Xu, Bao-yun,Zhang, You-jun. 2012

[7]Microarray analysis of the gene expression profile in the midgut of silkworm infected with cytoplasmic polyhedrosis virus. Wu, Ping,Wang, Xiu,Jiang, Yun-Feng,Guo, Xi-Jie,Wu, Ping,Qin, Guang-xing,Liu, Ting,Li, Mu-Wang,Guo, Xi-Jie.

[8]CDNA cloning and characterization of LASP1 from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan.

[9]Cytoplasmic polyhedrosis virus-induced differential gene expression in two silkworm strains of different susceptibility. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie.

[10]Cloning and expression analysis of a peptidoglycan recognition protein in silkworm related to virus infection. Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan.

[11]Molecular cloning and characterization of biphenyl hydrolase-like (BPHL) protein gene from silkworm, Bombyx mori. Gao, Kun,Deng, Xiang Yuan,Gao, Kun,Shang, Meng Ke,Qian, He Ying,Guo, Xi Jie,Gao, Kun,Shang, Meng Ke,Qian, He Ying,Guo, Xi Jie. 2016

[12]Differentially expressed genes in resistant and susceptible Bombyx mori strains infected with a densonucleosis virus. Bao, Yan-Yuan,Ge, Jun-Qing,Zhang, Chuan-Xi,Li, Mu-Wang,Zhao, Yun-Po,Wang, Cheng-Shu,Huang, Yong-Ping. 2008

[13]Early responses of silkworm midgut to microsporidium infection - A Digital Gene Expression analysis. Yue, Ya-Jie,Tang, Xu-Dong,Xu, Li,Yan, Wei,Li, Qian-Long,Xiao, Sheng-Yan,Fu, Xu-Liang,Wang, Wei,Li, Nan,Shen, Zhong-Yuan,Yue, Ya-Jie,Tang, Xu-Dong,Xu, Li,Yan, Wei,Li, Qian-Long,Xiao, Sheng-Yan,Fu, Xu-Liang,Wang, Wei,Li, Nan,Shen, Zhong-Yuan,Yue, Ya-Jie,Tang, Xu-Dong,Xiao, Sheng-Yan,Li, Nan,Shen, Zhong-Yuan.

[14]Identification of differentially expressed genes during anther abortion of Taigu Genic Male Sterile Wheat by combining suppression subtractive hybridization and cDNA array. Chang, Qing-Shan,Zhou, Rong-Hua,Kong, Xiu-Ying,Yu, Zeng-Liang,Jia, Ji-Zeng. 2006

[15]Identification of stage-specifically expressed genes of Trichinella spiralis by suppression subtractive hybridization. Liu, M. Y.,Wang, X. L.,Fu, B. Q.,Li, C. Y.,Wu, X. P.,Le Rhun, D.,Chen, Q. J.,Boireau, P.. 2007

[16]Isolation and Analysis of Drought-Induced Genes in Maize Roots. Li Hui-yong,Huang Shu-hua,Shi Yun-su,Song Yan-chun,Zhong Zhong-bao,Wang Guo-ying,Wang Tian-yu,Li Yu,Li Hui-yong. 2009

[17]Gene expression profiling of resistant and susceptible Bombyx mori strains reveals nucleopolyhedrovirus-associated variations in host gene transcript levels. Bao, Yan-Yuan,Tang, Xu-Dong,Lv, Zu-Yao,Tian, Cai-Hong,Xu, Yi Peng,Zhang, Chuan-Xi,Wang, Xiu-Ye. 2009

[18]Identification and characterization of genes related to the development of breast muscles in Pekin duck. Xu, Tieshan,Huang, Wei,Hou, Shuisheng,Xu, Tieshan,Zhou, Hanlin,Zhang, Xiaohui,Ye, Baoguo.

[19]Development of a specific molecular tool for the detection of epidemiologically active mulberry causing-disease strains of Ralstonia solanacearum phylotype I (historically race 5-biovar 5) in China. Xu, J.,Xu, J. S.,Zhang, H.,Chen, K. Y.,Tian, Q.,Zhang, L. Q.,Liu, L.,He, L. Y.,Feng, J.,Prior, P.,Pan, Z. C..

[20]Gene transcription analysis during interaction between potato and Ralstonia solanacearum. Li, G. C.,Jin, L. P.,Wang, X. W.,Xie, K. Y.,Huang, S. W.,Qu, D. Y.,Li, G. C.,Yang, Y.,van der Vossen, E. A. G.. 2010

作者其他论文 更多>>