数字农科院2.0

Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification

文献类型： 外文期刊

作者： Chen, Hao-tai;Zhang, Jie;Yang, Sheng-hai;Ma, Li-na;Ma, Yan-ping;Liu, Xiang-tao;Cai, Xue-peng;Zhang, Yong-guang;Liu, Yong-sheng

作者机构：

关键词： Porcine parvovirus (PPV);Detection;Loop-mediated isothermal amplification (LAMP);Sensitivity;Specificity

期刊名称： JOURNAL OF VIROLOGICAL METHODS

ISSN： 0166-0934

年卷期： 2009 年 158 卷 1-2 期

页码：

收录情况： SCI

摘要： A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine parvovirus (PPV) DNA. The amplification could be finished in 45 min under isothermal condition at 62 degrees C by employing a set of four primers targeting VP2 gene of PPV. LAMP assay showed higher sensitivity than PCR, with a detection limit of 5 copies of PPV genomic DNA per reaction. No cross reactivity was observed from the samples of other related viruses including canine parvovirus, parvovirus B19, porcine circovirus type 1, porcine circovirus type 2 and porcine peudorabies virus. The detection rate of PPV LAMP for 125 clinical samples was 97.6% and appeared higher than that of PCR method. The result indicated the potential usefulness of the technique as a simple, rapid procedure for the detection of PPV. (c) 2009 Elsevier B.V. All rights reserved.

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