Characterization of phytoene synthase 1 gene (Psy1) located on common wheat chromosome 7A and development of a functional marker
文献类型： 外文期刊
作者： He, X. Y.;He, Z. H.;Wu, Y. P.;Xiao, Y. G.;Xia, X. C.;Zhang, Y. L.;Ma, C. X.;He, Z. H.
作者机构：
期刊名称： THEORETICAL AND APPLIED GENETICS
ISSN： 0040-5752
年卷期： 2008 年 116 卷 2 期
页码：
收录情况： SCI
摘要： Phytoene synthase (Psy), a critical enzyme in the carotenoid biosynthetic pathway, demonstrated high association with the yellow pigment (YP) content in wheat grain. Characterization of Psy genes and the development of functional markers for them are of importance for marker-assisted selection in wheat breeding. In this study, the full-length genomic DNA sequence of a Psy gene (Psy-A1) located on chromosome 7A, was characterized by in silico cloning and experimental validation. The cloned Psy-A1 comprises six exons and five introns, 4,175 bp in total, and an ORF of 1,284 bp. A co-dominant marker, YP7A, was developed based on polymorphisms of two haplotypes of Psy-A1, yielding 194 and 231-bp fragments in cultivars with high and low YP content, respectively. The marker YP7A was mapped on chromosome 7AL using an RIL population from cross PH82-2/Neixing 188, and a set of Chinese Spring nullisomic-tetrasomic lines and ditelosomic line 7AS. Psy-A1, co-segregating with the STS marker YP7A, was linked to SSR marker Xwmc809 on chromosome 7AL with a genetic distance of 5.8 cM, and explained 20-28% of the phenotypic variance for YP content across three environments. A total of 217 Chinese wheat cultivars and advanced lines were used to validate the association between the polymorphic band pattern and grain YP content. The results showed that the functional marker YP7A was closely related to grain YP content and, therefore, could be used in wheat breeding programs targeting of YP content for various wheat-based products.
分类号：
- 相关文献
作者其他论文 更多>>
-
Subtraction suppressive hybridisation analysis of differentially expressed genes associated with puberty in the goat hypothalamus
作者：Cao, G. L.;Feng, T.;Chu, M. X.;Di, R.;Huang, D. W.;Liu, Q. Y.;Hu, W. P.;Wang, X. Y.;Cao, G. L.;Zhang, Y. L.;Feng, T.
关键词：bioinformatic analysis;carbohydrate metabolism;energy metabolism;GNAS;Gria 3;onset of puberty;oxidative phosphorylation;subtracted cDNA library
-
Characterization of DFR allelic variations and their associations with pre-harvest sprouting resistance in a set of red-grained Chinese wheat germplasm
作者：Bi, H. H.;Sun, Y. W.;Xiao, Y. G.;Xia, L. Q.
关键词：Triticum aestivum;Grain color;Dihydroflavonol-4-reductase gene;Pre-harvest sprouting;Gene expression
-
Mapping quantitative trait loci for pre-harvest sprouting resistance in white-grained winter wheat line CA 0431
作者：Miao, X. L.;Zhang, Y. J.;Xia, X. C.;He, Z. H.;Zhang, Y.;Yan, J.;Chen, X. M.;He, Z. H.
关键词：ICIM;microsatellite markers;pre-harvest sprouting;QTL mapping;Tritium aestivum
-
Quantitative trait loci of stripe rust resistance in wheat
作者：Rosewarne, G. M.;Herrera-Foessel, S. A.;Singh, R. P.;Lan, C. X.;He, Z. H.;Huerta-Espino, J.;He, Z. H.
关键词：
-
Pyramiding adult-plant powdery mildew resistance QTLs in bread wheat
作者：Bai, B.;He, Z. H.;Asad, M. A.;Lan, C. X.;Zhang, Y.;Xia, X. C.;Yan, J.;Chen, X. M.;Bai, B.;Wang, C. S.;He, Z. H.;Bai, B.
关键词：adult-plant resistance gene;Blumeria graminis f. sp tritici;gene combinations;molecular markers;Triticum aestivum
-
Breeding Adult Plant Resistance to Stripe Rust in Spring Bread Wheat Germplasm Adapted to Sichuan Province of China
作者：Zou, Y. C.;Yang, W. Y.;Tang, Y. L.;He, Z. H.;Singh, R. P.
关键词：durable resistance;Puccinia striiformis;stripe rust;Triticum aestivum
-
Allelic variants of phytoene synthase 1 (Psy1) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for flour colour
作者：He, X. Y.;He, Z. H.;Xia, X. C.;He, Z. H.;Ma, W.;Appels, R.
关键词：Common wheat;Yellow pigment content;Phytoene synthase 1 gene;1B.1R translocation;Allelic variation