数字农科院2.0

Detection of homozygosity in near isogenic Lines of non-susceptible to Zhenjiang strain of densonucleosis virus in silkworm

文献类型： 外文期刊

作者： Li Muwang;Hou Chengxiang;Zhao Yunpo;Xu Anying;Guo Xijie;Huang Yongping

作者机构：

关键词： silkworm;non-susceptible to DNVZ;near isogenic line;SSR marker;homozygosity and linkage group

期刊名称： AFRICAN JOURNAL OF BIOTECHNOLOGY

ISSN： 1684-5315

年卷期： 2007 年 6 卷 14 期

页码：

收录情况： SCI

摘要： The near isogenic lines (NILs) of non-susceptible to DNV-Z were bred through backcrossing successively using L10 as donor parent and Js as recurrent parent. The homozygosity of the NILs was detected using the SSR markers from the SSR linkage map. The results showed that the ratio of the linkage groups from the recurrent parent in the NILs increased rapidly along with the increase of the backcrossing generations. After 6 generations of backcrossing, it was selfed and was fed with DNV-Z. The linkage groups of 9 of the 5 BC6 pairs, whose 1/4 offspring were non-susceptible to DNV-Z, were all replaced by the recurrent parent except the linkage group that held nsd-Z, and only part of one linkage group from the other one individual had not been replaced. According to the markers linked to nsd-Z, FI0316 had not been replaced in all of the 10 individuals, but crossover happened in 2 individuals between FI0568 and nsd-Z.

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