Cloning of fatty acid elongase 1 gene and molecular identification of A and C genome in Brassica species
文献类型: 外文期刊
作者: Wu Yuhua;Xiao Ling;Wu Gang;Lu ChangMing
作者机构:
关键词: brassica;fatty acid elongase 1;A and C genome identification;AvrII
期刊名称: SCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN: 1006-9305
年卷期: 2007 年 50 卷 3 期
页码:
收录情况: SCI
摘要: The fatty acid elongase 1 (FAE1) genes of Brassic napus were cloned from two cultivars, i.e. Zhongshuan No. 9 with low erucic acid content, and Zhongyou 821 with high erucic acid content, using the degenerate PCR primers. The sequence analysis showed that there was no intron within the FAE1 genes. The FAE1 genes from Zhongyou 821 contained a coding sequence of 1521 nucleotides, and those cloned from ZhongE;huan No. 9 contained a 1517 bp coding sequence. Alignment of the FAE1 sequences from Brassica rapa, B. oleracea and B. napus detected 31 single nucleotide polymorphic sites (2.03%), which resulted in 7 amino-acid substitutions. Further analysis indicated that 19 SNPs were genome-specific, of which, 95% were synonymous mutations. The nucleotide substitution at position 1217 in the FAE1 genes led to a specific site of restricted cleavage. An AvrII cleavage site was present only in the C genome genes and absent in the A genome FAE1 genes. Digestion profile of the FAE1 sequences from B. rapa, B. oleracea and B. napus produced with AvrII confirmed that the FAE1 genes of B. oleracea origin was recognized and digested, while that of B. rapa origin could not. The results indicated that by AvrII cleavage it was possible to distinguish B. rapa from B. oleracea and between the A and C genome of B. napus. In addition, the FAE1 genes could be used as marker genes to detect the pollen flow of P. napus, thus providing an alternative method for risk assessment of gene flow.
分类号:
- 相关文献
作者其他论文 更多>>
-
Coupled system for underground heating exchange and solar heat-humidity regulation in greenhouse: Experimental study and simulation analysis
作者:Chen Xinge;Zang Jianbin;Wu Gang;Liang Hao;Yang Yunfan;Shi Dawei;Feng Chaoqing
关键词:Composite desiccant;Greenhouse;Heat and humidity control;Underground heat exchange
-
Direct utilization of solar linear Fresnel reflector on multi-effect eccentric horizontal tubular still with falling film
作者:Wu Gang; Yang Qichang;Zheng Hongfei; Zhang Yi;Fang Hui; Jin Rihui
关键词:Falling water film;Heat and mass transfer;Multi-eccentric;Horizontal tubular still;Solar distillation
-
Aberrant Meiotic Prophase I Leads to Genic Male Sterility in the Novel TE5A Mutant of Brassica napus
作者:Yan Xiaohong;Zeng Xinhua;Wang Shasha;Li Keqi;Yuan Rong;Gao Hongfei;Luo Junling;Liu Fang;Wu Yuhua;Li Yunjing;Zhu Li;Wu Gang
关键词:
-
Molecular evidence for blocking erucic acid synthesis in rapeseed (Brassica napus L.) by a two-base-pair deletion in FAE1 (fatty acid elongase 1)
作者:Wu Lei;Jia Yan-li;Wu Gang;Lu Chang-ming
关键词:erucic acid;fatty acid elongase 1;natural mutation;Brassica napus L.
-
Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events
作者:Wu Yu-hua;Zhang Li;Wu Gang;Nie Shu-jing;Lu Chang-ming;Zhang Li
关键词:transgenic rapeseed;junction fragment;pre-insertion site;DNA rearrangement
-
Rationalizing the isolation distance needed for field trials involving genetically modified rapeseed (Brassica napus L.) in China
作者:Zhao XiangXiang;Lu ChangMing;Zhao XiangXiang;Tang Tang;Chen GuiMin;Liu FuXia;Wang XingLong;Bu CuiPing
关键词:regulated isolation distance;genetically modified winter rapeseed;pollen flow rate;tolerance threshold
-
Effects of elevated CO2 on the nutrient compositions and enzymes activities of Nilaparvata lugens nymphs fed on rice plants
作者:Wu Gang;Zhuang Jing;Zhao WanYun;Hua HongXia;Huang WenKun;Su Li;Li JunSheng;Xiao NengWen;Xiong YanFei
关键词:Nilaparvata lugens;elevated CO2;rice plants;total superoxide dismutase;detoxification enzyme;glutathione S-transferase