文献类型： 外文期刊

作者： Zhuo Renying *; Qiao Guirong ; Sun Zongxiu

作者机构：

关键词： Chinese sweetgum;plantlet regeneration;transgenic plantlet;salt induced promoter

期刊名称： Plant Cell, Tissue and Organ Culture

ISSN： 0167-6857

年卷期： 2007 年 88 卷 1 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Conventional breeding of Chinese sweetgum is constrained by its long-reproductive cycle, which includes long-juvenile periods, and by its complex reproductive characteristics, including self-incompatibility and a high degree of heterozygosis. Like other tree species, sweetgum has undergone relatively little domestication; the methodology described here in illustrates the possibility of transforming Liquidambar formosana L. obtained from leafy explants using Agrobacter tumefaciens. PCR and Southern blotting show that foreign gene had integrated to genomic DNA. The results indicated that superoxide dismutase (SOD) and peroxidase (POD) activities increased with the stress time in all treated plants and these activities of the transgenic plant were stably higher than those of the control. RT-PCR showed that BADH expressed strongly induced by NaCl. The present study showed that the Rd29A promoter is able to direct osmotant gene expression when plant was exposed to salt, cold, and drought stress, with the advantage that expression was absent or undetectable in natural grow phase.

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