数字农科院2.0

Efficient Agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection

文献类型： 外文期刊

作者： Ding Zai-Song;Zhao Ming;Jing Yu-Xiang;Li Liang-Bi;Kuang Ting-Nin

作者机构：

关键词： Agrobacterium-mediated transformation;mannose selection;transgenic rice

期刊名称： PLANT MOLECULAR BIOLOGY REPORTER

ISSN： 0735-9640

年卷期： 2006 年 24 卷 3-4 期

页码：

收录情况： SCI

摘要： A positive selection system was developed for Agrobacterium-mediated transformation of rice that does not use toxic Compounds such as antibiotics or herbicides. The selection system is based on the Escherichia coli phosphomannose isomerase (pmi) gene as a selectable marker and mannose as the selective agent. Only transgenic plants were able to metabolize mannose into a usable Source of carbon, fructose. Selection was achieved using a combination of mannose and sucrose at 10 g/L and 5 g/L, respectively. Transgenic rice plants were produced efficiently in japanica rice variety Zhonghua 8, with transformation frequency of 16.5%, which was slightly lower than that achieved by hygromycin selection.

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