数字农科院2.0

Flow injection-chemiluminescence determination of soybean isoflavone

文献类型： 外文期刊

作者： Dan, Yang;Jing, Cang;Hao Zaibin;Qiu Lijuan

作者机构：

关键词： flow injection;chemiluminescence;soybean isoflavone

期刊名称： CHINESE JOURNAL OF ANALYTICAL CHEMISTRY

ISSN： 0253-3820

年卷期： 2006 年 34 卷 8 期

页码：

收录情况： SCI

摘要： A flow injection-chemiluminescence ( CL) method based on the chemiluminescence reaction of soybean isoflavone and hexacyanoferrate (fff) in NaOH solution was developed for the determination of soybean isoflavones. The optimal conditions of CL system were 5.0 x 10(-4) mol/L KFe (CN)(6) 0.5 mol/L NaOH, and peristaltic pump 145 r/min and sub-peristaltic pump (35 r/min) were choiled. The method has a linear range of 1.0 x 10(-3) -0.5 g/L. Regression equation: A (apex area) = 193305C (mg/L) + 229.97, r = 0.9962. The detection limit is 4.6 x 10(-4) g/L. The relative standard deviation was 2.5 % (c = 5.0 x 10(-3) g/L, n=7). The method was successfully applied to the determination of soybean isoflavone in biologic manufacture.

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