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The effect of bovine IFN-alpha on the immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus

文献类型： 外文期刊

作者： Guo, HC;Liu, ZX;Sun, SQ;Leng, QW;Li, D;Liu, XT;Xie, QG

作者机构：

关键词： foot-and-mouth disease virus (FMDV);DNA vaccine;IFN-alpha;gene vaccination

期刊名称： ACTA BIOCHIMICA ET BIOPHYSICA SINICA

ISSN： 1672-9145

年卷期： 2004 年 36 卷 10 期

页码：

收录情况： SCI

摘要： In this study, we constructed recombinant plasmid pcDNA3.1/P12X3C3D including Pl, 2A, 3C, 31) and part of 2B gene of FMDV and pcDNA3.l/IFN containing the gene encoding bovine IFN-alpha. We inoculated the DNA vaccine pcDNA3.1/P12X3C3D with or without pcDNA3.1/IFN to evaluate the efficiency of this DNA vaccine and the immunogenicity of DNA vaccine enhanced by the co-delivery with pcDNA3.1/IFN. After two times of vaccination with DNA vaccine, all of guinea pigs were challenged with 10(3) ID50 FMDV type O. Anti-FMDV antibody levels were detected by ELISA and T lymphocyte proliferation response was tested by MTT assay. The result shows that guinea pigs inoculated by pcDNA3.1/P12X3C3D alone or with pcDNA3.1/IFN generated specific antibodies and induced an FMDV-specific T lymphocyte proliferate m response. FMDV challenge tests showed that one in four guinea pigs immunized by pcDNA3.1/Pl2X3C3D with pcDNA3.1/IFN was protected from the FMDV serotype O infection. This result indicated that the efficiency of the DNA vaccine was enhanced by co-delivery with pcDNA3.1/IFN. However, the protection rate was considerably lower than that immunized with conventional FMD vaccine.

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