数字农科院2.0

Screening of molecular markers for NPV resistance in Bombyx mori L. (Lep., Bombycidae)

文献类型： 外文期刊

作者： Yao, Q;Li, MW;Wang, Y;Wang, WB;Lu, J;Dong, Y;Chen, KP

作者机构：

期刊名称： JOURNAL OF APPLIED ENTOMOLOGY-ZEITSCHRIFT FUR ANGEWANDTE ENTOMOLOGIE

ISSN： 0931-2048

年卷期： 2003 年 127 卷 3 期

页码：

收录情况： SCI

摘要： Silkworm (Bombyx mori L.) is one of the important economic insects. Silkworm rearing and silk industry plays an important role in China, India and other developing countries. In the long history of sericultural practice, introduction of silkworm strains with high resistance to diseases has greatly improved cocoon and silk quality and productivity. However, current silkworm breeding is mainly based on traditional method that involves high input of time and labour. In order to increase the selection efficiency and accuracy for future silkworm breeding, it is necessary to establish a molecular marker-assisted selection system. In our study, three silkworm near isogenic lines that had different resistance to nuclear polyhedrosis virus (NPV) were established by means of different hybridization methods. A total of 150 random amplified polymorphic DNA (RAPD) random primers were used to screen molecular markers. Among them, two molecular markers OPA-18(700) and OPY-11(400) were found linked to major genes resistant and susceptible to NPV, respectively. Validity of the molecular markers was proved in F2 populations.

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