数字农科院2.0

Cloning and sequence analysis of a gene encoding polygalacturonase-inhibiting protein from cotton

文献类型： 外文期刊

作者： Dou, DL;Wang, BS;Tang, YX;Wang, ZX;Sun, JS

作者机构：

关键词： Gossypium barbadense;polygalacturonase-inhibiting protein ( pgip) gene;rapid amplification of cDNA ends (RACE);sequence analysis;leucine-rich repeats (LRRs)

期刊名称： PROGRESS IN NATURAL SCIENCE

ISSN： 1002-0071

年卷期： 2003 年 13 卷 2 期

页码：

收录情况： SCI

摘要： Polygalacturonase-inhibiting proteins (PGIP) play important roles in plant defense of pathogen, especially fungi. A pair of degenerated primers is designed based on the conserved sequence of 20 other known pgip genes and used to amplify Gossypium barbadense cultivation 7124 cDNA library by touch-down PCR. A 561 bp internal fragment of the pgip gene is obtained and used to design the primers for rapid amplification of cDNA ends. A composite pgip gene sequence is constructed from the products of 5' and 3' RACE, which are 666 bp and 906 bp respectively. Analysis of nucleic acid sequence shows 69.2% and 68.7% similarity to Citrus and Poncirus pgip genes, respectively. Its open reading frame of the gene encodes a polypeptide of 330 amino acids, in which 10 leucine-rich repeats arrange tandemly. A new set of primers is designed to the 5' and 3' ends of the gene, which allows amplification of the full-length gene from the cotton cDNA library. Genomic DNA analysis reveals that this gene has no intron.

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