数字农科院2.0

A genome-specific repetitive DNA sequence from Oryza eichingeri: characterization, localization, and introgression to O-sativa

文献类型： 外文期刊

作者： Yan, HH;Liu, GQ;Cheng, ZK;Li, XB;Liu, GZ;Min, SK;Zhu, LH

作者机构：

关键词： repetitive DNA sequence;Oryza eichingeri;fluorescent in situ hybridization;introgression lines

期刊名称： THEORETICAL AND APPLIED GENETICS

ISSN： 0040-5752

年卷期： 2002 年 104 卷 2-3 期

页码：

收录情况： SCI

摘要： In the course of transferring the brown plant-hopper resistance from a diploid, CC-genome wild rice species, OryZa cichingeri (IRGC ace. 105159 and 105163), to the cultivated rice variety 02428, we have isolated many alien addition and introgression lines. The O. eichingeri chromatin in some of these lines has previously been identified using genomic in situ hybridization and molecular-marker analysis. Here we cloned a tandemly repetitive DNA sequence from O. eichingeri IRGC acc105163, and detected it in 25 introgression lines. This repetitive DNA sequence showed high specificity to the rice CC genome, but was absent from all the four tetraploid species with BBCC or CCDD genomes. The monomer in this repetitive DNA sequence is 325-366-bp long, with a copy number of about 5,000 per I C of the O. eichingeri genome, showing 88% homology to a repetitive DNA sequence isolated from Oryza officinalis (2n=2x=24, CC). Fluorescent in situ hybridization revealed 11 signals distributed over eight O. cichingeri chromosomes, mostly in terminal or subterminal regions.

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