数字农科院2.0

Isolation and genetic characterization of a fragile plant mutant in rice (Oryza sativa L.)

文献类型: 外文期刊

作者: Qian Qian ; Li Yunhai +; Zeng Dali ; Teng Sheng ; Wang Zhengke ; Li Xueyong ; Dong Zhigang ; Dai Ning ; Sun Lei ; Li Jiayang *

作者机构:

关键词: Oryza sativa L.;fragile plant locus (fpl);molecular markers

期刊名称: Chinese Science Bulletin

ISSN: 1001-6538

年卷期: 2001 年 46 卷 24 期

页码:

收录情况: SCI

摘要: The fragile rice mutant was isolated from an M-2 population of indica variety Shuang Ke Zao (SKZ) treated with x-rays, and designated as fp1 (fragile plant 1) because of its fragile leaves and culms. To map FP1 locus, an F-2 mapping population was derived from a cross between the fp1 and C-bao, a polymorphic japonic variety. The primary mapping result places the FP1 locus in an interval between two molecular markers, microsatellite marker RM16 (3.1 cM proximal to FP1) and STS marker G144a (9.1 cM distal to FP1) in the centromere region of chromosome 3. A CAPS marker C524a was further developed between RM16 and G144a, with 0.4 cM genetic distances to the FP1 locus, providing a practical starting point for constructing a BAC contig spanning the FP1 locus and cloning the fp1 gene Allelism test demonstrated that fp1 is allelic to bc1, a fragile rice mutant reported previously.

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