FphA Positively Regulates Fungal Development and AFB1 Biosynthesis and Phosphorylates Ypd1 in Aspergillus flavus
文献类型: 外文期刊
作者: Tian, Yuanyuan;Zhuo, Qiuhan;Sun, Lu;Li, Xu;Wang, Gang;Xing, Fuguo
作者机构:
关键词: aflatoxin;aflatoxin biosyntheticregulation;fphA;phosphorylation
期刊名称: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN: 0021-8561
年卷期: 2025 年
页码:
收录情况: SCIE(2025版) ; ; EI(2025版)
摘要: Aspergillus flavus is a ubiquitous fungus capable of producing aflatoxin B1 (AFB1), a potent carcinogen that threatens food safety and human health. Here, we identified and characterized histidine kinase FphA as a positive regulator of fungal development and AFB1 biosynthesis. Deletion of fphA impaired mycelial growth and markedly reduced AFB1 production, accompanied by downregulation of cluster regulators aflR and aflS and multiple structural genes. Transcriptomic and phosphoproteomic analyses revealed significant transcriptional and phosphorylation changes in MAPK signaling components (e.g., Hog1 and SskA) in the Delta fphA strain. Although yeast two-hybrid assays showed no direct interaction between FphA and MAPK proteins, in vitro kinase assays demonstrated that FphA phosphorylates phosphotransfer protein Ypd1, suggesting regulation via the FphA-Ypd1-MAPK cascade. These findings establish FphA as a key regulator linking environmental sensing with fungal development and toxin biosynthesis, providing potential molecular targets for controlling aflatoxin contamination in agricultural products.
分类号:
- 相关文献
作者其他论文 更多>>
-
Lactic Acid Bacteria as the Green and Safe Food Preservatives: Their Mechanisms, Applications and Prospects
作者:Zhang, Yuwei;Li, Lianrui;Pang, Xiaoyang;Zhang, Shuwen;Liu, Yang;Wang, Yunna;Xie, Ning;Li, Xu
关键词:lactic acid bacteria (LAB);food preservatives;antimicrobial mechanism;food control;prospects
-
Solid-State Fermentation of Acid-Resistant Aspergillus niger A515: An Approach to Degrade AFB1 and ZEN and Enhance Nutritional Components in Maize Products
作者:Gao, Yuan;Sun, Lu;Tian, Xiaoyu;Yang, Shuo;Du, Haolan;Zhao, Haiyan;Yang, Bolei;Xing, Fuguo
关键词:solid-state fermentation;Aspergillus niger;maize-based products;mycotoxins;nutritional components;antinutritional factors
-
From hemorrhage to apoptosis: understanding the devastating impact of ASFV on piglets
作者:Xu, Fanghua;Li, Ning;Xue, Yue;Wang, Zhenru;Fang, Zheng;An, Hui;Liu, Sidang;Weng, Changjiang;Huang, Li;Wang, Gang
关键词:ASFV;multiple organ lesions;pathogenicity;apoptosis;piglets
-
Differential Retention and Loss of a Mycotoxin in Fungal Evolution
作者:Chen, Lin;Yan, Ziying;Yang, Bolei;Tai, Bowen;Li, Weizhao;Li, Erfeng;Wang, Gang;Xing, Fuguo
关键词:ochratoxin;gene cluster;evolution;degeneration
-
Metarhizium spp. encode an ochratoxin cluster and a high efficiency ochratoxin-degrading amidohydrolase revealed by genomic analysis
作者:Wang, Gang;Wu, Wenqing;Keller, Nancy P.;Guo, Xu;Li, Erfeng;Ma, Junning;Xing, Fuguo
关键词:Secondary metabolite;Mycotoxin;OTA;Detoxification;Ochratoxigenic fungi
-
Exploring the Mechanism of Benzyl Isothiocyanate in the Prevention and Control of Ceratocystis fimbriata and the Role of Its Response Protein PRX1
作者:Wang, Shan;Geng, Qingru;Zhuo, Qiuhan;Ye, Yan;Li, Shuolong;Zhang, Ninghui;Wang, Bo;Yang, Kunlong;Tian, Jun
关键词:sweetpotato black rot;benzyl isothiocyanate;proteomics;bioinformatics;PRX1
-
Effect of heat-resistant protease from psychrotrophic bacteria in raw milk on shelf-life quality of UHT milk
作者:Xu, Jinyu;Hei, Xue;Zheng, Mumin;Wang, Yunna;Xie, Ning;Li, Xu;Wang, Xiaodan;Lv, Jiaping;Lu, Gang;Pang, Xiaoyang;Yu, Dongwei;Zhang, Shuwen
关键词:UHT milk;Heat-resistant protease;Quality deterioration;Shelf life