数字农科院2.0

FphA Positively Regulates Fungal Development and AFB1 Biosynthesis and Phosphorylates Ypd1 in Aspergillus flavus

文献类型: 外文期刊

作者: Tian, Yuanyuan;Zhuo, Qiuhan;Sun, Lu;Li, Xu;Wang, Gang;Xing, Fuguo

作者机构:

关键词: aflatoxin;aflatoxin biosyntheticregulation;fphA;phosphorylation

期刊名称: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN: 0021-8561

年卷期: 2025 年

页码:

收录情况: SCIE(2025版) ; ; EI(2025版)

摘要: Aspergillus flavus is a ubiquitous fungus capable of producing aflatoxin B1 (AFB1), a potent carcinogen that threatens food safety and human health. Here, we identified and characterized histidine kinase FphA as a positive regulator of fungal development and AFB1 biosynthesis. Deletion of fphA impaired mycelial growth and markedly reduced AFB1 production, accompanied by downregulation of cluster regulators aflR and aflS and multiple structural genes. Transcriptomic and phosphoproteomic analyses revealed significant transcriptional and phosphorylation changes in MAPK signaling components (e.g., Hog1 and SskA) in the Delta fphA strain. Although yeast two-hybrid assays showed no direct interaction between FphA and MAPK proteins, in vitro kinase assays demonstrated that FphA phosphorylates phosphotransfer protein Ypd1, suggesting regulation via the FphA-Ypd1-MAPK cascade. These findings establish FphA as a key regulator linking environmental sensing with fungal development and toxin biosynthesis, providing potential molecular targets for controlling aflatoxin contamination in agricultural products.

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