数字农科院2.0

OSNRT1.1B-OSCNGC14/16-CA2+-OSNLP3 Pathway: Phosphorylation-Mediated Maintenance of Nitrogen Homeostasis

文献类型： 外文期刊

作者： Wang, Xiaohan;Liu, Yongqiang;Li, Weiwei;Ma, Xiaojun;Wang, Wei;Jiang, Zhimin;Wang, Yiqin;Li, Legong;Hu, Bin;Chu, Chengcai

作者机构：

关键词： Ca2(+) signaling;nitrate Signaling;nitrogen homeostasis;phosphorylation;rice

期刊名称： ADVANCED SCIENCE

ISSN：

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Nitrate, a crucial nutrient and signaling molecule, is extensively studied across plants. While the NRT1.1-NLP-centered pathway dominates nitrate signaling in Arabidopsis and rice, however, whether there is functional interaction or co-regulation between the primary nitrate response (PNR) and long-term nitrogen utilization remains unclear. Here, a novel nitrate signaling pathway is identified in rice that works alongside the established ubiquitination-mediated OsNRT1.1B-OsSPX4-OsNLP3 cascade. It is demonstrated that OsCNGC14, OsCNGC16, and OsNRT1.1B form a plasma membrane-localized complex in root tips, mediating nitrate-triggered Ca2(+) influx. The absence of either OsCNGC14 or OsCNGC16 abolished Ca2(+) signaling and suppressed PNR. The OsNRT1.1B-OsCNGC14/16 complex activates Ca2(+)-dependent phosphorylation of OsNLP3 at Ser193, which accelerates its nuclear translocation and transcriptional activation of nitrate-responsive genes. This phosphorylation enhances both short-term PNR and long-term nitrogenutilization. This findings reveal a dual regulatory network in rice: the Ca2(+)-OsNLP3 pathway rapidly amplifies nitrate signals, while the ubiquitination-mediated OsSPX4 degradation ensures sustained nitrogen homeostasis.

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