数字农科院2.0

Methyl Jasmonate- and Salicylic Acid-Induced Transcription Factor ZjWRKY18 Regulates Triterpenoid Accumulation and Salt Stress Tolerance in Jujube

文献类型： 外文期刊

作者： Cuiping Wen;Zhong Zhang;Qianqian Shi;Xiaoshan Duan;Jiangtao Du;Cuiyun Wu;Xingang Li

作者机构：

关键词： jujube (Ziziphus jujuba Mill.);overexpression;salt tolerance;silencing;triterpenoid biosynthesis;ZjWRKY18

期刊名称： International Journal of Molecular Sciences

ISSN： 1661-6596

年卷期： 2023 年 24 卷 4 期

页码：

收录情况： SCIE（2023版）

摘要： Triterpenoids are important, pharmacologically active substances in jujube (Ziziphus jujuba Mill.), and play an important role in the plant’s resistance to abiotic stress. However, regulation of their biosynthesis, and the underlying mechanism of their balance with stress resistance, remain poorly understood. In this study, we screened and functionally characterized the ZjWRKY18 transcription factor, which is associated with triterpenoid accumulation. The transcription factor is induced by methyl jasmonate and salicylic acid, and its activity was observed by gene overexpression and silencing experiments, combined with analyses of transcripts and metabolites. ZjWRKY18 gene silencing decreased the transcription of triterpenoid synthesis pathway genes and the corresponding triterpenoid content. Overexpression of the gene promoted the biosynthesis of jujube triterpenoids, as well as triterpenoids in tobacco and Arabidopsis thaliana. In addition, ZjWRKY18 binds to W-box sequences to activate promoters of 3-hydroxy-3-methyl glutaryl coenzyme A reductase and farnesyl pyrophosphate synthase, suggesting that ZjWRKY18 positively regulates the triterpenoid synthesis pathway. Overexpression of ZjWRKY18 also increased tolerance to salt stress in tobacco and Arabidopsis thaliana. These results highlight the potential use of ZjWRKY18 to improve triterpenoid biosynthesis and salt stress tolerance in plants, and provide a strong basis for metabolic engineering to improve the content of triterpenoids and breeding of jujube varieties that are resistant to stress.

分类号：

  • 相关文献

[1]A High-K+ Affinity Transporter (HKT) from Actinidia valvata Is Involved in Salt Tolerance in Kiwifruit. Shichao Gu,Shiming Han,Muhammad Abid,Danfeng Bai,Miaomiao Lin,Leiming Sun,Xiujuan Qi,Yunpeng Zhong,Jinbao Fang. 2023

[2]Overexpression of MsNIP2 improves salinity tolerance in Medicago sativa. Weiye Kong,Haijun Huang,Wenxuan Du,Zhihu Jiang,Yijing Luo,Dengxia Yi,Guofeng Yang,Yongzhen Pang. 2024

[3]大麦黄矮病毒GAV株系编码的基因沉默抑制因子的鉴定. 王锡锋,周广和. 2006

[4]Identification And Characterization Of A N.ovel Emaravirus Associated With J ujube (Ziziphus Jujuba Mill.) Yellow Mottle Disease. Cao, Mengji,Di Serio, Francesco,Cao, Mengji,Yang, Caixia,Han, Tong,Yang, Caixia,Fu, Jingjing,Zhang, Song,Zhang, Song. 2019

[5]The Effect of Bone Morphogenetic Protein 2 or Extracellular Signal-Regulated Kinase 1 Silencing on Phosphorus Utilization and Related Parameters in Primary Broiler Osteoblasts. Yanqiang Geng,Tingting Li,Yun Hu,Liyang Zhang,Xiaoyan Cui,Ling Zhu,Bingxin Wu,Xugang Luo. 2022

[6]GhHB14_D10 and GhREV_D5, two HD-ZIP III transcription factors, play a regulatory role in cotton fiber secondary cell wall biosynthesis. Li S.,Yu M.,Qanmber G.,Feng M.,Hussain G.,Wang Y.,Yang Z.,Zhang J.. 2024

[7]A wheat R2R3-MYB gene, TaMYB30-B, improves drought stress tolerance in transgenic Arabidopsis. Zhang, Lichao,Zhao, Guangyao,Xia, Chuan,Jia, Jizeng,Liu, Xu,Kong, Xiuying. 2012

[8]Overexpression of the novel Zygophyllum xanthoxylum C2H2-type zinc finger gene ZxZF improves drought tolerance in transgenic Arabidopsis and poplar. Chu, Yanguang,Zhang, Weixi,Huang, Qinjun,Zhang, Bingyu,Su, Xiaohua,Chu, Yanguang,Zhang, Weixi,Huang, Qinjun,Zhang, Bingyu,Su, Xiaohua,Wu, Bin.

[9]Overexpression of a Lotus corniculatus AP2/ERF transcription factor gene, LcERF080, enhances tolerance to salt stress in transgenic Arabidopsis. Sun, Zhan-Min,Zhou, Mei-Liang,Tang, Yi-Xiong,Wu, Yan-Min,Sun, Zhan-Min,Xiao, Xing-Guo.

[10]CkDREB gene in Caragana korshinskii is involved in the regulation of stress response to multiple abiotic stresses as an AP2/EREBP transcription factor. Wang, Xuemin,Chen, Xiaofang,Gao, Hongwen,Wang, Zan,Sun, Guizhi,Liu, Yun.

[11]The wheat calcium-dependent protein kinase TaCPK7-D positively regulates host resistance to sharp eyespot disease. Wei, Xuening,Shen, Fangdi,Hong, Yantao,Rong, Wei,Du, Lipu,Liu, Xin,Xu, Huijun,Ma, Lingjian,Zhang, Zengyan,Shen, Fangdi,Ma, Lingjian.

[12]Overexpression of a wheat MYB transcription factor gene, TaMYB56-B, enhances tolerances to freezing and salt stresses in transgenic Arabidopsis. Zhang, Lichao,Zhao, Guangyao,Xia, Chuan,Jia, Jizeng,Liu, Xu,Kong, Xiuying.

[13]Molecular cloning and characterization of three isoprenyl diphosphate synthase genes from alfalfa. Sun, Yan,Zhou, He,Zhang, Ze,Kang, Junmei,Zhang, Tiejun,Yang, Qingchuan.

[14]Overexpression of sugarcane gene SoSnRK2.1 confers drought tolerance in transgenic tobacco. Sun, Bo,Niu, Jun-Qi,Tan, Qin-Liang,Li, Jian,Yang, Li-Tao,Li, Yang-Rui,Yang, Li-Tao,Li, Yang-Rui.

[15]Correlation between AS1 gene expression and seed protein contents in different soybean (Glycine max [L.] merr.) cultivars. Wan, TF,Shao, GH,Shan, XC,Zeng, NY,Lam, HM.

[16]Transcript characteristic of myostatin in sheep fibroblasts. Lu, Jian,Ding, Jiatong,Li, Bichun,Lu, Jian,Zhang, Xiaoning,Zhao, Fuping,Zhou, Xinlei,Zhang, Li,Wei, Caihong,Du, Lixin,Ren, Hangxing,Sheng, Xihui,Li, Shangang.

[17]Isolation and functional characterization of salt-stress induced RCI2-like genes from Medicago sativa and Medicago truncatula. Long, Ruicai,Zhang, Fan,Li, Zhenyi,Cong, Lili,Kang, Junmei,Zhang, Tiejun,Yang, Qingchuan,Li, Mingna,Sun, Yan,Zhao, Zhongxiang,Zhao, Zhongxiang.

[18]A putative leucine-rich repeat receptor kinase, OsBRR1, is involved in rice blast resistance. Peng, Hao,Zhang, Qian,Sun, Xuehui,Lu, Tiegang,Zhang, Qian,Zhai, Ying,Sun, Daye,Sun, Ying,Li, Yadong,Lei, Cailin.

[19]High-yield production of a chitinase from Aeromonas veronii B565 as a potential feed supplement for warm-water aquaculture. Zhang, Yuting,Zhou, Zhigang,Liu, Yuchun,Cao, Yanan,He, Suxu,Huo, Fengmin,Qin, Chubin,Yao, Bin,Ringo, Einar.

[20]Gene Cloning, Overexpression, and Characterization of a Xylanase from Penicillium sp CGMCC 1669. Liu, Wanli,Shi, Pengjun,Yang, Peilong,Wang, Guozeng,Wang, Yaru,Luo, Huiying,Yao, Bin,Liu, Wanli,Chen, Qiang. 2010

作者其他论文 更多>>