数字农科院2.0

Cryo-EM structure of a nanobody-bound heliorhodopsin

文献类型: 外文期刊

作者: Ruixue Xia;Mingxia Sun;Yang Lu;Na Wang;Anqi Zhang;Changyou Guo;Zhenmei Xu;Xuehui Cai;Yuanzheng He

作者机构:

关键词: Acetate;Cryo-EM;Heliorhodopsin;Nanobody

期刊名称: Biochemical and Biophysical Research Communications

ISSN: 0006-291X

年卷期: 2025 年 750 卷

页码:

收录情况: SCIE(2025版)

摘要: Heliorhodopsins (HeRs) represent a distinct class of microbial rhodopsins (MRs) with an inverted membrane topology compared to other MRs. Previous structural studies have shown that HeRs lack a proton acceptor residue, and protons are never released from the protein. In this study, we present the cryo-electron microscopy (cryo-EM) structure of HeR bound to a nanobody. The structure reveals an acetate-like molecule in the Schiff base cavity (SBC) on the intracellular side of HeR under neutral condition. Structural comparisons and analyses suggest that the acetate molecule may function as a proton acceptor for the protonated retinal Schiff base (RSB) and act as a mediator for the intramolecular signaling transduction in HeR during light stimulation. These structural insights shed new light on the mechanism and function of HeR.

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