文献类型: 外文期刊
作者: Ruixue Xia;Mingxia Sun;Yang Lu;Na Wang;Anqi Zhang;Changyou Guo;Zhenmei Xu;Xuehui Cai;Yuanzheng He
作者机构:
关键词: Acetate;Cryo-EM;Heliorhodopsin;Nanobody
期刊名称: Biochemical and Biophysical Research Communications
ISSN: 0006-291X
年卷期: 2025 年 750 卷
页码:
收录情况: SCIE(2025版)
摘要: Heliorhodopsins (HeRs) represent a distinct class of microbial rhodopsins (MRs) with an inverted membrane topology compared to other MRs. Previous structural studies have shown that HeRs lack a proton acceptor residue, and protons are never released from the protein. In this study, we present the cryo-electron microscopy (cryo-EM) structure of HeR bound to a nanobody. The structure reveals an acetate-like molecule in the Schiff base cavity (SBC) on the intracellular side of HeR under neutral condition. Structural comparisons and analyses suggest that the acetate molecule may function as a proton acceptor for the protonated retinal Schiff base (RSB) and act as a mediator for the intramolecular signaling transduction in HeR during light stimulation. These structural insights shed new light on the mechanism and function of HeR.
分类号:
- 相关文献
作者其他论文 更多>>
-
A Study of the Different Strains of the Genus Azospirillum spp. on Increasing Productivity and Stress Resilience in Plants
作者:Wenli Sun;Mohamad Hesam Shahrajabian;Na Wang
关键词:Azospirillum;Bacillus;biostimulant;plant growth-promoting rhizobacteria
-
Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1
作者:Le Wang;Ruifang Jia;Jianjun Hao;Zhengqiang Chen;Xiaoluo Zhang;Na Wang;Shengze Wang;Yuanyuan Zhang;Kejian Lin
关键词:
-
Development of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) mRNA Vaccine against Highly Pathogenic PRRSV Challenge
作者:Mirwaise Khan;Xinqi Shi;Ziyi Wei;Fandan Meng;Peiyu Xiao;Tao Wang;Lingzhi Luo;Dasong Xia;Tongqing An;Haiwei Wang;Xuehui Cai
关键词:Lipid nanoparticles;Mixed immunization;mRNA vaccine;Porcine reproductive and respiratory syndrome virus;Swine industry
-
Genetic evolution and alterations in pathogenicity of highly pathogenic porcine reproductive and respiratory syndrome virus
作者:Chao Li;Hu Xu;Jinhao Li;Bangjun Gong;Zhenyang Guo;Lirun Xiang;Siyu Zhang;Qi Sun;Jing Zhao;Menglin Zhang;Chaoliang Leng;Kuan Zhao;Jianan Wu;Qian Wang;Jinmei Peng;Guohui Zhou;Huairan Liu;Tongqing An;Xuehui Cai;Zhi Jun Tian;Yan Dong Tang;Hongliang Zhang
关键词:genetic evolution;HP-like PRRSV;HP-PRRSV;pathogenicity alterations;vaccine-like
-
Effect of L-theanine combined with geniposide on depression-like behavior induced by the chronic unpredictable mild stress (CUMS) in mice
作者:Qiming Yu;Yan Zhu;Tao Jiang;Ying Gao;Nan Ling;Na Wang;John Shi;Weichun Pan;Qizhen Du;Junfeng Yin
关键词:CUMS;Depression;Geniposide;L-Theanine;Transthyretin
-
Development of blocking ELISA for detection anti-PRRSV antibodies and serological investigation of PRRSV in China
作者:Shujie Wang;Tianfeng Chen;Yong Bo Yang;Hongfeng Wang;Mingxia Sun;Kexin Gao;Ziyi Wei;Yuanzhe Bai;Yan Dong Tang;Xuehui Cai;Tongqing An
关键词:b-ELISA;Monoclonal antibodies;Nucleocapsid (N) protein;Porcine reproductive and respiratory syndrome virus
-
A lineage 1 branch porcine reproductive and respiratory syndrome virus live vaccine candidate provides broad cross-protection against HP-like PRRSV in piglets
作者:Chao Li;Jinhao Li;Bangjun Gong;Hu Xu;Zhenyang Guo;Lirun Xiang;Siyu Zhang;Qi Sun;Jing Zhao;Menglin Zhang;Yan Dong Tang;Chaoliang Leng;Jianan Wu;Qian Wang;Jinmei Peng;Guohui Zhou;Huairan Liu;Tongqing An;Xuehui Cai;Zhi Jun Tian;Hongliang Zhang
关键词:cross-protective efficacy;HP-like PRRSV;Lineage 1 branch;live vaccine