数字农科院2.0

Determination of thiram by a peptide-Cu mimetic enzyme with peroxidase-like activity

文献类型： 外文期刊

作者： Yunling Shao;Miao Wang;Jianpeng Zhang;Yongxin She;Zhen Cao;Guohua Zhang;Fen Jin;Jing Wang;A. M. Abd El-Aty

作者机构：

关键词： Peptide-Cu mimetic enzyme;Pesticides;POD activity;Thiram

期刊名称： Food Chemistry

ISSN： 0308-8146

年卷期： 2026 年 505 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： In this study, we constructed a peptide‑copper (peptide-Cu) mimetic enzyme for thiram detection. The enzyme mimics peroxidase-like activity (POD), which allows it to catalyze the oxidation of o -phenylenediamine (OPD) substrates in the presence of hydrogen peroxide (H₂O₂). Thiram can prevent the catalytic activity of peptide-Cu mimetic enzymes by surface blocking, inhibiting chromogenic substrate oxidation and facilitating the rapid detection of thiram. The inhibitory mechanism was elucidated through molecular dynamics (MD) simulations and density functional theory (DFT) calculations, revealing that thiram synergistically reduces the catalytic activity of the peptide-Cu mimetic enzyme by disrupting its structural stability and electronic properties. Thiram exhibited good linearity in the concentration range from 0.01 to 5 μg/mL, with a lowest limit of detection (LOD) of 0.048 μg/mL, and showed a good recovery range from 79.71% to 108.13% in real samples. This approach, based on peptide-metal complexes, offers a straightforward and affordable method for pesticide monitoring.

分类号：

  • 相关文献

[1]Effective duration of pesticide-induced susceptibility of rice to brown planthopper (Nilaparvata lugens Stal, Homoptera : Delphacidae), and physiological and biochemical changes in rice plants following pesticide application. Wu, JC,Qiu, HM,Yang, GQ,Liu, JL,Liu, GJ,Wilkins, RM. 2004

[2]Opportunities of Knowledge and Technology Exchange between China and Europe to Solve Common Groundwater Management Problems Related to Agriculture. Wilko, Schweers. 2008

[3]Simultaneous Determination of 70 Pesticide Residues in Coffees by Gas Chromatography-Mass Spectrometry. Yang, Xin,Wang, Jing,Yang, Xin,Zhang, Hua,Xu, Dechang,Qiu, Jianwei,Wang, ZhenYu,Cao, WeiQiang. 2010

[4]Study on multiple pesticides by High Performance Liquid Chromatography-Mass Spectrometry using modified QuEChERS method - A case study on CHM. Dong, Yiwei,Bai, Wei,Li, Yuzhong,Xu, Chunying,Fang, Fuli,Guo, Zhicheng. 2012

[5]Multiresidue determination of 114 multiclass pesticides in flue-cured tobacco by solid-phase extraction coupled with gas chromatography and tandem mass spectrometry. Cao, Jianmin,Sun, Na,Yu, Weisong,Pang, Xueli,Lin, Yingnan,Kong, Fanyu,Qiu, Jun.

[6]Novel acetylcholinesterase inhibitors: Synthesis and structure-activity relationships of phthalimide alkyloxyphenyl N,N-dimethylcarbamate derivatives. Yang, Guoquan,Mei, Xiangdong,Yuan, Huizhu,Ning, Jun,Zhao, Qianfei.

[7]Multiresidue method for determination of 88 pesticides in berry fruits using solid-phase extraction and gas chromatography-mass spectrometry: Determination of 88 pesticides in berries using SPE and GC-MS. Yang, X.,Zhang, H.,Zhang, Y. C.,Dong, A. J.,Zhao, H. T.,Cui, J.,Liu, Y.,Yang, X.,Wang, J.,Sun, C. H..

[8]Simultaneous determination of 118 pesticide residues in Chinese teas by gas chromatography-mass spectrometry. Yang, Xin,Xu, De Chang,Qiu, Jian Wei,Zhang, Hua,Zhang, Ying Chun,Dong, Ai Jun,Ma, Ying,Wang, Jing,Wang, Jing.

[9]Evaluation of surface-enhanced Raman scattering detection using a handheld and a bench-top Raman spectrometer: A comparative study. Zheng, Jinkai,Pang, Shintaro,He, Lili,Zheng, Jinkai,Labuza, Theodore P..

[10]Transfer Rates of 19 Typical Pesticides and the Relationship with Their Physicochemical Property. Chen, Hongping,Pan, Rong,Zhang, Minglu,Liu, Xin,Lu, Chengyin,Chen, Hongping,Pan, Meiling,Liu, Xin,Lu, Chengyin,Chen, Hongping,Liu, Xin,Lu, Chengyin.

[11]Pesticide productivity, host-plant resistance and productivity in China. Widawsky, D,Rozelle, S,Jin, SQ,Huang, JK. 1998

[12]Determination of Organophosphorus Pesticides Based on Biotin-Avidin Enzyme Linked Immunosorbent Assay. Wang Lei,Zhang Li-Jie,Lue Wei,Han Shi-He,Zhang Fu-Kai,Pan Jia-Rong,Han Shi-He. 2011

[13]Stereoselective determination of benalaxyl in plasma by chiral high-performance liquid chromatography with diode array detector and application to pharmacokinetic study in rabbits. Wang, Qiuxia,Zhu, Wentao,Jia, Guifang,Wang, Xinquan,Zhou, Zhiqiang.

[14]Enantioselective effects of metalaxyl on soil enzyme activity. Yue, Heng,Fang, Song,Zhang, Yizhi,Ning, Yang,Yu, Weisong,Kong, Fanyu,Qiu, Jun,Yue, Heng,Fang, Song,Zhang, Yizhi,Ning, Yang,Yu, Weisong,Kong, Fanyu,Qiu, Jun.

[15]Comparison of different cleanup procedures for oil crops based on the development of a trace analytical method for the determination of pyraclostrobin and epoxiconazole. Dong, Fengshou,Xu, Jun,Liu, Xingang,Cheng, Youpu,Chen, Zenglong,Liu, Na,Chen, Xixi,Tao, Yan,Zheng, Yongquan.

[16]Field-Testing of Synthetic Herbivore-Induced Plant Volatiles as Attractants for Beneficial Insects. Zhang, Yongjun,Wu, Kongming,Guo, Yu Yuan,Yu, Huilin.

[17]Lethal and Sublethal Effects of Endosulfan on Apolygus lucorum (Hemiptera: Miridae). Lu, Yanhui,Wu, Kongming,Liu, Yangqing,Xue, Fangsen,Wyckhuys, Kris A. G..

[18]Simultaneous Determination of 69 Pesticide Residues in Coffee by Gas Chromatography-Mass Spectrometry. Yang, Xin,Xu, D. C.,Qiu, J. W.,Ma, Ying,Cui, J.,Yang, Xin,Wang, Jing. 2011

[19]Ubi1 intron-mediated enhancement of the expression of Bt cry1Ah gene in transgenic maize (Zea mays L.). Lang Zhihong,Huang DaFang,Zhang Jie,He KangLai,Song FuPing.

[20]Residue behaviour of six pesticides in button crimini during home canning. Liu, Xingang,Dong, Fengshou,Xu, Jun,Li, Yuanbo,Zheng, Yongquan,Gu, Xiaojun,Kong, Zhiqiang. 2014

作者其他论文 更多>>