数字农科院2.0

Methanosuratincola petrocarbonis gen. nov., sp. nov., a methyl-reducing methanogen isolated from Shengli oil field, and proposal of Methanosuratincolaceae fam. nov., Methanosuratincolales ord. nov. and Methanosuratincolia classis nov. in the phylum Thermoproteota

文献类型： 外文期刊

作者： Kejia Wu;Lei Zhou;Fengfeng Zheng;Laiyan Liu;Min Yang;Jiang Li;Diana Z. Sousa;Lei Cheng

作者机构：

关键词： methanogen;Methanosuratincolia;methyl-reducing methanogenesis;oil field;‘Candidatus Methanomethylicia’

期刊名称： International Journal of Systematic and Evolutionary Microbiology

ISSN： 1466-5026

年卷期： 2025 年 75 卷 7 期

页码：

收录情况： SCIE（2025版）

摘要： An anaerobic, thermophilic methanogen, designated strain LWZ-6T, was isolated from the Shengli oil field, China. The cells of strain LWZ-6T were non-motile cocci, with a diameter of 0.5–1.0 µm, and formed aggregates. They reduced methanol and monomethylamine into methane, using H2 as an electron donor. Dimethylamine, trimethylamine and methanethiol, H2 /CO2, formate, acetate, pyruvate, lactate and glucose were not used as energy sources. Strain LWZ-6T required yeast extract, acetate or CO2 as carbon sources. Strain LWZ-6T grew at 35–65 °C (optimum 55 °C), pH 5.0–8.0 (optimum 6.0–6.5) and 0–60 g l−1 NaCl (optimum 9 g l−1). The genome was 1.54 Mbp with a G+C content of 54.42 mol%. Strain LWZ-6T shared 83.54% 16S rRNA gene sequence identity with Infirmifilum lucidum strain 3507LTT in the class Thermoprotei. Phylogenetic analysis based on the 16S rRNA gene, along with phylogenomic analysis, indicated that strain LWZ-6T belonged to the candidate class ‘Candidatus Methanomethylicia’, which lacks cultivated representatives. Based on these findings, a new species within a new genus, Methanosuratincola petrocarbonis gen. nov., sp. nov., is proposed for LWZ-6T (=CCAM 1872T=JCM 39528T). In addition, we propose the Methanosuratincolia class. nov. for candidate class ‘Ca. Methanomethylicia’ represented by Methanosuratincolaceae fam. nov. and Methanosuratincolales ord. nov. within the phylum Thermoproteota.

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