数字农科院2.0

Single-nucleus RNA sequencing defines adipose tissue subpopulations that contribute to Tibetan pig cold adaptation

文献类型： 外文期刊

作者： Jiali Liu;Yao Jiang;Tianxia Liu;Chuanhe Chen;Linya Chui;Along Cui;Xueping Zhang;Xiao Wang;Yu Wang;Chunhuai Yang;Ying Zhang;Tianwen Wu;Shulin Yang;Jiaojiao Huang;Cong Tao;Jianguo Zhao;Yanfang Wang

作者机构：

关键词： Adipocytes;Cold stimulation;Pig;snRNA-seq;Subpopulations

期刊名称： BMC Biology

ISSN： 1741-7007

年卷期： 2025 年 23 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Background: Thermogenic beige adipocyte displays a remarkable ability in mammals to adapt to cold environments, but the underlying cellular mechanisms remain unclear, especially in pigs that lack functional UCP1. Results: Multilocular beige adipocytes were observed in both Tibetan pigs (cold-tolerant) and Bama pigs (cold-sensitive) after short-term cold exposure (4 ℃ for 3 days). Through single-nucleus RNA sequencing of adipose tissues, including subcutaneous inguinal adipose tissues (IAT) and perirenal adipose tissues (PAT), from both pig breeds at room temperature and cold treatment conditions, we discovered two cell subpopulations specific to Tibetan pigs, PDGFRα+EBF2High in IAT and ADIPOQ+HIF1AHigh in both depots. PDGFRα+EBF2High cells were characterized as potential beige precursors, while ADIPOQ+HIF1AHigh cells were found to express highly thermogenic-related genes. Despite the decrease of the lipogenic subpopulation and the increase of the lipolytic and the thermogenic subpopulations observed in both pig breeds upon cold treatment, Tibetan pigs exhibited stronger cellular and molecular responses compared to Bama pigs. Remarkably, cold-induced de novo beige adipogenesis and white adipocyte browning, likely occurred in Tibetan pigs, while Bama pigs relied more heavily on white browning. Moreover, BMP7, which was highly expressed in the PDGFRα+EBF2High subpopulation, positively regulates porcine beige thermogenic capacity. Conclusions: Our data offers a comprehensive and unprecedented perspective on the heterogeneity and plasticity of adipose tissues of pigs and broadens the understanding of beige fat biology in mammals.

分类号：

  • 相关文献

[1]Piceatannol antagonizes lipolysis by promoting autophagy-lysosome-dependent degradation of lipolytic protein clusters in adipocytes. Jung Yeon Kwon,Jonathan Kershaw,Chih Yu Chen,Susan M. Komanetsky,Yuyan Zhu,Xiaoxuan Guo,Phillip R. Myer,Bruce Applegate,Kee Hong Kim. 2022

[2]CircRNA Profiling Reveals an Abundant circBDP1 that Regulates Bovine Fat Development by Sponging miR-181b/miR-204 Targeting Sirt1/TRARG1. Zhang, Sihuan,Jiang, Enhui,Kang, Zihong,Bi, Yi,Liu, Hongfei,Xu, Han,Wang, Zhen,Lei, Chuzhao,Chen, Hong,Lan, Xianyong. 2022

[3]Individual-breed assignment analysis in swine populations by using microsatellite markers. Fan, B,Chen, YZ,Moran, C,Zhao, SH,Liu, B,Yu, M,Zhu, MJ,Xiong, TA,Lil, K. 2005

[4]Genome-Wide Association Study for Certain Carcass Traits and Organ Weights in a Large WhitexMinzhu Intercross Porcine Population. Liu Xin,Wang Li-gang,Liang Jing,Yan Hua,Zhao Ke-bin,Li Na,Zhang Long-chao,Wang Li-xian,Li Na. 2014

[5]Molecular characterization and association analysis of FBXO40 with partial hematological indexes in pig. Wang, Z. W.,Li, X. Y.,Tang, Z. L.,Yang, S. L.,Ying, Z. Z.,Fu, T.,Mu, Y. L.,Ao, H.,Li, K.,Wang, Z. W.,Li, X. Y.,Ying, Z. Z.,Fu, T.,Fan, B.. 2010

[6]The microRNA expression profile in porcine skeletal muscle is changed by constant heat stress. Hao, Y.,Liu, J. R.,Yang, P. G.,Feng, Y. J.,Cui, Y. J.,Yang, C. H.,Gu, X. H.,Liu, J. R.,Zhang, Y..

[7]Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size. Liang Jing,Li Na,Zhang Long-chao,Wang Li-gang,Liu Xin,Zhao Ka-bin,Yan Hua,Pu Lei,Zhang Yue-bo,Shi Hui-bi,Wang Li-xian,Li Na,Zhang Qin. 2016

[8]Genetic Variation of EPAS1 Gene in Tibetan Pigs and Three Low-Altitude Pig Breeds in China. Dong Kun-zhe,Yao Na,Shu Guo-tao,Zhao Qian-jun,Ma Yue-hui,Kang Ye,Zuo Qing-qing. 2014

[9]Effect of Graded Levels of Fiber from Alfalfa Meal on Nutrient Digestibility and Flow of Fattening Pigs. Chen Liang,Gao Li-xiang,Zhang Hong-fu. 2014

[10]Seroprevalence and genotypes of Toxoplasma gondii isolated from pigs intended for human consumption in Liaoning province, northeastern China. Wang, Dawei,Zhang, Guoxin,Yuan, Gaoming,He, Jianbin,Yang, Na,Liu, Yan,Jiang, Tiantian,Su, Chunlei. 2016

[11]Chromosome Mapping, Expression and Polymorphism Analysis of CRABP1 Gene in Pigs. Zhao Shuan-ping,Tang Zhong-lin,Zhou Rong,Li Kui,Zheng Jian-wei,Qu Chang-qing,Zhao Shuan-ping. 2014

[12]Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs. Chen Liang,Gao Li-xiang,Liu Li,Ding Ze-ming,Zhang Hong-fu,Chen Liang. 2015

[13]Effect of graded levels of fiber from alfalfa meal on intestinal nutrient and energy flow, and hindgut fermentation in growing pigs. Chen, L.,Zhang, H. F.,Gao, L. X.,Zhao, F.,Lu, Q. P.,Sa, R. N.. 2013

[14]Effects of Long-Term Protein Restriction on Meat Quality, Muscle Amino Acids, and Amino Acid Transporters in Pigs. Yin, Jie,Li, Yuying,Zhu, Xiaotong,Han, Hui,Ren, Wenkai,Chen, Shuai,Bin, Peng,Liu, Gang,Li, Tiejun,Yin, Yulong,Yin, Jie,Li, Yuying,Zhu, Xiaotong,Han, Hui,Ren, Wenkai,Chen, Shuai,Bin, Peng,Liu, Gang,Li, Tiejun,Yin, Yulong,Yin, Jie,Li, Yuying,Zhu, Xiaotong,Han, Hui,Ren, Wenkai,Chen, Shuai,Bin, Peng,Liu, Gang,Li, Tiejun,Yin, Yulong,Yin, Jie,Li, Yuying,Zhu, Xiaotong,Han, Hui,Ren, Wenkai,Chen, Shuai,Bin, Peng,Liu, Gang,Li, Tiejun,Yin, Yulong,Yin, Jie,Li, Yuying,Han, Hui,Ren, Wenkai,Chen, Shuai,Bin, Peng,Huang, Xingguo,Fang, Rejun,Huang, Xingguo,Fang, Rejun,Li, Tiejun,Yin, Yulong,Wang, Bin,Wang, Kai,Sun, Liping.

[15]Timing of The First Zygotic Cleavage as a Developmental Potential Marker for Porcine Cloned Embryos. Luo Xue-Ming,Feng Chong,Long Chuan,Yan Jun,Pan Deng-Ke,Xiao Wei,Xue Zhen-Hua,Yun Peng. 2010

[16]Genome-wide profiling of Sus scrofa circular RNAs across nine organs and three developmental stages. Liang, Guoming,Yang, Yalan,Niu, Guanglin,Tang, Zhonglin,Li, Kui,Liang, Guoming,Yang, Yalan,Tang, Zhonglin,Li, Kui,Liang, Guoming,Yang, Yalan,Tang, Zhonglin.

[17]Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China. Wang Shuai,Zhang Meng,Liu Xin-chao,Lin Tao,Zhao Guang-wei,Ia Hassan,Yan Ruo-feng,Song Xiao-kai,Li Xiang-rui,Yang Han-chun,Lin Tao,Yuan Shi-shan. 2015

[18]Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP. Jiang, Hai-Hai,Huang, Si-Yang,Zhou, Dong-Hui,Zhang, Xiao-Xuan,Su, Chunlei,Zhu, Xing-Quan,Deng, Shun-Zhou,Su, Chunlei,Zhang, Xiao-Xuan. 2013

[19]Genome-wide association study for rib eye muscle area in a Large WhitexMinzhu F-2 pig resource population. Guo Yun-yan,Liu Wen-zhong,Guo Yun-yan,Zhang Long-chao,Wang Li-xian. 2015

[20]Energy, amino acid, and phosphorus digestibility of phytase transgenic corn for growing pigs. Li, S. F.,Niu, Y. B.,Feng, M. S.,Du, B.,Liu, J. S.,Lu, L.,Zhang, L. Y.,Ran, C. Y.,Luo, X. G.,Liu, J. S.,Deng, J. L.. 2013

作者其他论文 更多>>