数字农科院2.0

Exploring the Anti-Inflammatory Effects and Molecular Mechanisms of Novel Peptides from Coregonus Peled: An Integrated Approach Combining In Silico and In Vitro

文献类型： 外文期刊

作者： Li, Ruohan;Liu, Shuyan;Zhang, Rong;Ma, Zhuang;Li, Yi;Gao, Yanan

作者机构：

关键词： Coregonus peled;anti-inflammatory peptides;network pharmacology;proteomics;molecular docking;cAMP/PKA/CREB pathway

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2026 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Inflammation is a key pathological process in chronic diseases. This study investigated the anti-inflammatory potential of bioactive peptides from Coregonus peled, a cold-water fish rich in protein. Among the six hydrolysates, Neutrase hydrolysates (NH) showed superior efficacy in reducing inflammation in LPS-induced RAW 264.7 macrophages. Amino acid composition analysis suggested that NH could be used as a potential source of anti-inflammatory peptides. Utilizing a combined approach of peptidomics and bioinformatics, four novel peptides, FGPPGPPTRPS (P1), AMPPPVRP (P2), PPPPTIPR (P3), and DRPGPPGGP (P4), were identified within NH, with P2 exhibiting the most potent anti-inflammatory activity. Integrated analyses of network pharmacology, proteomics, and molecular docking indicated that the cAMP signaling pathway was involved in the anti-inflammatory effect of peptide P2. Further validation using the PKA inhibitor H-89 demonstrated that the peptide P2 exerted its anti-inflammatory effect by inhibiting the cAMP/PKA/CREB signaling pathway. These findings highlight the potential of C. peled-derived peptides for functional foods or pharmaceutical applications.

分类号：

  • 相关文献

[1]Network pharmacology and phytochemical composition combined with validation in vivo and in vitro reveal the mechanism of platycodonis radix ameliorating PM2.5-induced acute lung injury. Han, Xianlei,Zhang, Yue,Zhang, Fan,Li, Xiumei,Meng, Yanli,Huo, Jinhai,Chen, Mian,Liu, Fei,Wang, Weiming,Wang, Nan. 2024

[2]The Discovery of Potential Repellent Compounds for Zeugodacus cucuribitae (Coquillett) from Six Non-Favored Hosts. Yu Fu,Yupeng Chen,Yani Wang,Xinyi Fu,Shunda Jin,Chunyan Yi,Xue Bai,Youqing Lu,Wang Miao,Xingyu Geng,Xianli Lu,Rihui Yan,Zhongshi Zhou,Fengqin Cao. 2025

[3]Network Pharmacology and Molecular Docking: Exploring the Mechanism of Peppermint in Mastitis Prevention and Treatment in Dairy Cows. Xinyu Wang,Jiaxin Lai,Fei Xu,Mingchun Liu. 2025

[4]Application of Network Pharmacology, Molecular Docking, and In Vitro Experimental Evaluation to Decipher the Anti-Inflammatory Mechanisms of Cirsium japonicum. Jiaxue Wang,Hui Tao,Zhenlong Wang,Wei An,Ya Zhao,Bing Han,Jinquan Wang,Xiuzhu Sun,Xiumin Wang. 2024

[5]α-Glucosidase Inhibition-Guided Network Pharmacology and Molecular Docking Reveal the Antidiabetic Potential of Cichorium intybus as a Functional Food. Shah, Abdul Bari,Baiseitova, Aizhamal,Amzeyeva, Ulpan,Shang, Xiaofei,Jenis, Janar. 2025

[6]Modified Bufei decoction improves calf health by enhancing immunity, reducing inflammation, and modulating gut microbiota. Zhuo Yang,Peng Ji,Yongli Hua,Chenchen Li,Haochi Yang,Fanlin Wu,Xiuqin Wang,Yanming Wei,Jianpeng Yan. 2025

[7]In-depth profiling of Phenolics in strawberries and their potential modulatory effects on the oxidative stress-disease Axis: Insights from UHPLC-QTOF-MS-based untargeted metabolomics, network pharmacology, and molecular docking. Zhiyuan Zeng,Lei Xu,Jiayue Xu,Xiaoyu Tian,Liang Zhao,Xiaojun Liao. 2025

[8]Platelet Proteomics and Tissue Metabolomics Investigation for the Mechanism of Aspirin Eugenol Ester on Preventive Thrombosis Mechanism in a Rat Thrombosis Model. Qi Tao,Li Ping Fan,Ji Feng,Zhi Jie Zhang,Xi Wang Liu,Zhe Qin,Jian Yong Li,Ya Jun Yang. 2024

[9]The blockage of the electron transport chains caused by PFOA isomers induced metabolic damage of Arabidopsis leaves. Hao Wang,Jianxiong Mei,Zeying He,Bingjie Liu,Jishi Wang,Yue Geng,Yanwei Zhang. 2025

[10]Royal jelly proteins-derived anti-inflammatory peptides protected inflammation in DSS-induced colitis mice through Src/NF-κB signaling pathway. Jianying Guo,Ruihua Ye,Qingyun Guo,Fei Pan,Zixu Wang,Yaoxing Chen,Wenli Tian,Yulan Dong. 2025

[11]Artificial intelligence-driven discovery of bioactive peptides: Computational approaches and future perspectives. Liu, Xu,Guan, Feifei,Luo, Huiying,Yao, Bin,Tian, Jian. 2025

[12]Integration of Network Pharmacology and Molecular Docking to Analyse the Mechanism of Action of Oregano Essential Oil in the Treatment of Bovine Mastitis. Guangjie Cao,Jing Liu,Huan Liu,Xiaojie Chen,Na Yu,Xiubo Li,Fei Xu. 2023

[13]Antioxidant Effects and Potential Molecular Mechanism of Action of Limonium aureum Extract Based on Systematic Network Pharmacology. Zhen Yang,Yanan Mo,Feng Cheng,Hongjuan Zhang,Ruofeng Shang,Xuehong Wang,Jianping Liang,Yu Liu,Baocheng Hao. 2022

[14]Anti-Liver Fibrosis Activity and the Potential Mode of Action of Ruangan Granules: Integrated Network Pharmacology and Metabolomics. Shang, Xiaofei,Yuan, Huixin,Dai, Lixia,Liu, Yang,He, Jian,Chen, Huan,Li, Hongyan,Li, Xiuhui. 2022

[15]Based on network pharmacology and molecular docking to explore the protective effect of Epimedii Folium extract on cisplatin-induced intestinal injury in mice. Xia J.,Hu J.-N.,Wang Z.,Cai E.-B.,Ren S.,Wang Y.-P.,Lei X.-J.,Li W.. 2022

[16]Natural bioactive compounds as potential sources for alleviation and treatment of androgenetic alopecia: A research advance. Fang C.,Yang H.,Hui J.,Fan D.,Deng J.. 2024

[17]Screening of Active Substances Regulating Alzheimer’s Disease in Ginger and Visualization of the Effectiveness on 6-Gingerol Pathway Targets. Pan Y.,Li Z.,Zhao X.,Du Y.,Zhang L.,Lu Y.,Yang L.,Cao Y.,Qiu J.,Qian Y.. 2024

[18]Efficacy of Chlorogenic Acid in Treating Tripterygium Glycoside-Induced Asthenozoospermia in Rats and Its Possible Mechanisms. Long Chen,God’spower Bello-Onaghise,Mo Chen,Shunda Li,Yu Zhang,Haoran Wang,Qianwei Qu,Yanhua Li. 2025

[19]The Extract of Piper nigrum Improves the Cognitive Impairment and Mood in Sleep-Deprived Mice Through the JAK1/STAT3 Signalling Pathway. Dongyan Guan,Zhiying Hou,Bei Fan,Yajuan Bai,Honghong Wu,Jiawei Yu,Hui Xie,Zhouwei Duan,Fengzhong Wang,Qiong Wang. 2025

[20]Identifying the active components and mechanisms of Persicae Semen in treating androgenetic alopecia: Insights from network pharmacology and experimental evaluations. Xiaojuan Li,Xiaoli Liu,Bin Li,Ziyi Liu,Shiqi Guo,Sha Xiong,Ting Xiao,Siwen Jiao,Yu Chen,Yuehong Xu. 2025

作者其他论文 更多>>