数字农科院2.0

A molecular framework underlying low-nitrogen-induced early leaf senescence in Arabidopsis thaliana

文献类型： 外文期刊

作者： Hongmei Fan;Shuxuan Quan;Qing Ye;Lei Zhang;Wei Liu;Ning Zhu;Xiaoqi Zhang;Wenyuan Ruan;Keke Yi;Nigel M. Crawford;Yong Wang

作者机构：

关键词： APC/C;GDS1;nitrate signaling;nitrogen-deficiency-induced leaf senescence;NUE;PIF4/PIF5;ubiquitination

期刊名称： Molecular Plant

ISSN： 1674-2052

年卷期： 2023 年 16 卷 4 期

页码：

收录情况： SCIE（2023版） ; ; CSCD（2023-2024年度） ; ; 科技核心（2023版）

摘要： Nitrogen (N) deficiency causes early leaf senescence, resulting in accelerated whole-plant maturation and severely reduced crop yield. However, the molecular mechanisms underlying N-deficiency-induced early leaf senescence remain unclear, even in the model species Arabidopsis thaliana. In this study, we identified Growth, Development and Splicing 1 (GDS1), a previously reported transcription factor, as a new regulator of nitrate (NO3−) signaling by a yeast-one-hybrid screen using a NO3− enhancer fragment from the promoter of NRT2.1. We showed that GDS1 promotes NO3− signaling, absorption and assimilation by affecting the expression of multiple NO3− regulatory genes, including Nitrate Regulatory Gene2 (NRG2). Interestingly, we observed that gds1 mutants show early leaf senescence as well as reduced NO3− content and N uptake under N-deficient conditions. Further analyses indicated that GDS1 binds to the promoters of several senescence-related genes, including Phytochrome-Interacting Transcription Factors 4 and 5 (PIF4 and PIF5) and represses their expression. Interestingly, we found that N deficiency decreases GDS1 protein accumulation, and GDS1 could interact with Anaphase Promoting Complex Subunit 10 (APC10). Genetic and biochemical experiments demonstrated that Anaphase Promoting Complex or Cyclosome (APC/C) promotes the ubiquitination and degradation of GDS1 under N deficiency, resulting in loss of PIF4 and PIF5 repression and consequent early leaf senescence. Furthermore, we discovered that overexpression of GDS1 could delay leaf senescence and improve seed yield and N-use efficiency (NUE) in Arabidopsis. In summary, our study uncovers a molecular framework illustrating a new mechanism underlying low-N-induced early leaf senescence and provides potential targets for genetic improvement of crop varieties with increased yield and NUE.

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