数字农科院2.0

Study of the occurrence of toxic alkaloids in forage grass by liquid chromatography tandem mass spectrometry

文献类型： 外文期刊

作者： Mengjie Qie;Shuangyue Li;Chuntao Guo;Shuming Yang;Yan Zhao

作者机构：

关键词： Ergot alkaloids;Feed;Forage grass;LC-MS/MS;Pyrrolizidine alkaloids;Tropane alkaloids

期刊名称： Journal of Chromatography A

ISSN： 0021-9673

年卷期： 2021 年 1654.0 卷

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： The toxic alkaloids in forage grass present a serious health hazard to humans and livestock, especially ergot alkaloids (EAs), pyrrolizidine alkaloids (PAs) and tropane alkaloids (TAs). Hence, there is a need for a simultaneous method that allows these dangerous plant toxins to be determined. A simple and effective method was developed to determine fifteen toxic alkaloids (EAs, PAs and TAs) in forage grass using the QuEChERS method and liquid chromatography tandem mass spectrometry (LC-MS/MS). The developed method was validated with average recoveries ranging from 63.10 to 102.10%, and relative standard deviations lower than or equal to 6.39% were obtained. Good linearity over the concentration range of 10-600 µg/kg dry matter (DM) was observed for the target alkaloids. The determination coefficients R2 calculated for each of the matrix calibration curves were greater than 0.99. The limits of detection and quantification were 5 µg/kg DM and 10 µg/kg DM, respectively. The reproducibility of the method was verified in three laboratories: all of the mean recoveries of 15 alkaloids were higher than 60%, and the relative standard deviations in alkaloids between laboratories were all less than 14.24%. The proposed method was applied to analyse 134 forage grass samples from the meadow steppe of Inner Mongolia to monitor toxic alkaloids. A significant difference in the frequency of contamination was found between different herbage species and different regions.

分类号：

  • 相关文献

[1]Simultaneous determination of ergot alkaloids in swine and dairy feeds using ultra high-performance liquid chromatography-tandem mass spectrometry. Saranya Poapolathep,Narumol Klangkaew,Zhaowei Zhang,Mario Giorgi,Antonio Francesco Logrieco,Amnart Poapolathep. 2021

[2]Metabolic Effects of Elicitors on the Biosynthesis of Tropane Alkaloids in Medicinal Plants. Yuru Wen,Yiran Liao,Yueli Tang,Hongbo Zhang,Jiahui Zhang,Zhihua Liao. 2023

[3]Functional divergence of two arginine decarboxylase genes in tropane alkaloid biosynthesis and root growth in Atropa belladonna. Xiaoqiang Liu,Mei Yang,Jiahui Zhu,Junlan Zeng,Fei Qiu,Lingjiang Zeng,Chunxian Yang,Hongbo Zhang,Xiaozhong Lan,Min Chen,Zhihua Liao,Tengfei Zhao. 2024

[4]阿维菌素在柑桔和土壤中的残留及其消解动态. 张娇娇,刘炎,叶玉凤,赵其阳,彭震宇,焦必宁,王成秋. 2017

[5]基于分散固相萃取液相色谱-串联质谱法测定大米中8种真菌毒素. 孙伟华,杨欢,曹赵云,马有宁,秦美玲,柴爽爽,陈铭学. 2017

[6]大丽轮枝菌分泌蛋白提取方法比较. 肖红利,桂月晶,祁伟彦,陈捷胤,李蕾,徐明,戴小枫. 2014

[7]LC-MS/MS检测饲料中二甲氧苄啶、甲氧苄啶和二甲氧甲基苄啶方法的建立. 杨亚军,刘希望,李冰,孔晓军,李世宏,秦哲,杜文斌,李剑勇. 2015

[8]黄瓜根分泌物中的7;8-苯并黄酮及其对枯萎病发生的影响. 李世东,郭荣君. 2010

[9]液相色谱-串联质谱法检测鸡蛋中金刚烷胺残留. 汤晓艳,陈东宇,周剑,毛雪飞,邱静,王敏. 2014

[10]晋麦47幼苗中一个水分胁迫应答蛋白的SDS-PAGE和Nano LC-MS/MS鉴定. 范锋贵,张晓科,任万杰,王晓龙,叶石,付晓洁,王宏礼,朱建楚. 2012

[11]黄瓜根分泌物中的7,8-苯并黄酮及其对枯萎病发生的影响. 李世东,郭荣君. 2010

[12]玉米DDGS中抗生素残留分析. 姚婷,秦玉昌,吴宁鹏,彭丽,孙丹丹,谷旭. 2014

[13]荷斯坦奶牛瘤胃微生物脲酶的分离与鉴定. 赵圣国,王加启,刘开朗,李旦,于萍,卜登攀. 2010

[14]卷丹百合及其鳞茎中秋水仙碱含量的测定. 陈凯,王米,何菁琦,张赛奇,许璟. 2020

[15]利用多功能针式过滤器快速检测油菜和苹果中68种农药及代谢物残留. 贾琪,王纯强,马蕊,王宇彤,钱永忠,邱静. 2019

[16]农产品与饲料中T-2毒素免疫亲和柱净化-液相色谱串联质谱检测技术研究. 李静,马飞,李培武,张奇,丁小霞,张文. 2014

[17]LC-MS/MS测定青稞中氨基酸的含量及主成分分析. 王猛强,邱城,张唐伟,李腾飞,李壮,王淼,曹振,王珊珊,郑鹭飞,金茂俊,王静,邵华,金芬,佘永新. 2022

[18]QuEChERS联合LC-MS/MS测定土壤及蚯蚓中吡虫啉及异丙甲草胺13种代谢产物. 符芙蓉,赵丽霞,李晓晶,叶会科,孙扬,李永涛. 2023

[19]LC-MS/MS法测定橄榄油中7种聚酯低聚物. 李佳琦,冯佳宁,仇凯,李众,夏伊宁,吴刚. 2024

[20]蔬菜中啶虫脒残留免疫分析方法研究. 刘蓓蓓,吕军,张杰,刘广洋,翟荣启,陈鸽,徐东辉. 2025

作者其他论文 更多>>