数字农科院2.0

A Ferritin-Based Eg95 Nanoparticle Vaccine Adjuvanted with pCpG Eliciting Robust Immune Responses Against Cystic Echinococcosis in Mice Model

文献类型： 外文期刊

作者： Gao, Xintao;Zhu, Xizhou;Liu, Xingjian;Zhou, Chenghao;Shang, Yuting;Wu, Tong;Jia, Hong;Zhang, Zhifang;Li, Yinue;Xin, Ting

作者机构：

关键词： cystic echinococcosis;vaccine;combination adjuvant;ferritin nanoparticle;CpG

期刊名称： INTERNATIONAL JOURNAL OF NANOMEDICINE

ISSN： 1178-2013

年卷期： 2025 年 20 卷

页码：

收录情况： SCIE（2025版）

摘要： Introduction: Cystic echinococcosis (CE), a chronic disabling parasitic zoonosis, poses a great threat to public health and livestock production and causes huge economic losses globally. The commercial Quil-A-adjuvanted Eg95 vaccine was empirically effective for CE control; however, it is expensive and has side effects and insufficient immunity. Purpose: This study aimed to employ a novel adjuvant consisting of a delivery system and an immune potentiator and assess its adjuvanticity to Eg95 antigen, thereby developing a safe and cost-effective novel vaccine against the disease. Methods: A ferritin-based Eg95 nanoparticle antigen was prepared and then mixed with a plasmid containing the TLR9 agonist CpG to formulate a novel nanovaccine. The safety and efficacy of the vaccine were evaluated in vitro and in vivo. Results: The nanovaccine induced potent and enduring Eg95-specific humoral and cellular immune responses, as well as protective immunity-associated Th1 polarization supported by the higher ratios of IgG2a/IgG1 and IFN-gamma/IL-4. Meanwhile, this nanovaccines exhibited favorable safety and economic profiles. Conclusion: Our data demonstrated that the ferritin-CpG hybrid is a promising combination adjuvant to upgrade the traditional Quil-A and this combination adjuvant-based nanovaccine presents good potential as an alternative to the commercial one for practical CE control.

分类号：

  • 相关文献

[1]马流感灭活疫苗的研制. 郭巍,戚亭,相文华,王晓钧. 2015

[2]Cloning and Functional Analysis of the Full Length cDNA Sequence of Eukaryotic Translation Initiation Factor 5 in Schistosoma japonicum. Cheng Guofeng,Lin Jiaojiao,Sun Jun,Li Hao,Zhu Chuangang,Zhou Yuancong,Cai Youmin. 2005

[3]ProtectiveimmunityinducedbyaDNAvaccineexpressingeIF4AofToxoplasmagondiiagainstacutetoxoplasmosisinmice. 陈佳,SiyangHuang,ZhongyuanLi,ZiguoYuan,DonghuiZhou,EskildPetersen,NianzhangZhang,XingquanZhu. 2013

[4]传染性法氏囊病病毒反向遗传技术发展及其应用DevelopmentandApplicationoftheReverseGeneticTechnologiesforInfectiousBursalDiseaseVirus. 祁小乐1,王永强1,高立1,高宏雷1,高玉龙1,王笑梅1,2*QIXiaole1,WANGYongqiang1,GAOLi1,GAOHonglei1,GAOYulong1,WANGXiaomei1,2*. 2015

[5]运用反向遗传学培养产生高成长性的猪流感疫苗(英文). TaoYangMingLiuChunguoLiuYunZhangDafeiLiuHaoChenBiotechnologyAnimalInfluenzaLaboratoryoftheMinistryAgricultureandNationalKeyLaboratoryofVeterinary,HarbinVeterinaryResearchInstituteofCAAS,Harbin150001,China. 2007

[6]表达H3N8亚型马流感病毒重组禽痘病毒载体疫苗的研究. 戚亭,郭巍,王晓钧. 2015

[7]Poly(I:C) and CpG improve the assembly of foot-and-mouth disease virus-like particles and immune response in mice. Haiyun Liu,Zhidong Teng,Hu Dong,Jiajun Li,Sahibzada Waheed Abdullah,Yun Zhang,Jinen Wu,Huichen Guo,Shiqi Sun. 2023

[8]Baculovirus-expressed self-assembling SARS-CoV-2 nanoparticle vaccines targeting the S protein induce protective immunity in mice. Xingjian Liu,Haozhi Song,Jianmin Jiang,Xintao Gao,Yongzhu Yi,Yuting Shang,Jialei Li,Dan Li,Zhen Zeng,Yinü Li,Zhifang Zhang. 2023

[9]Immunoadjuvant efficacy of CpG plasmids for H9N2 avian influenza inactivated vaccine in chickens with maternal antibodies. Xue Pan,Qinfang Liu,Mart C.M. de Jong,Maria Forlenza,Shiqi Niu,Dawei Yan,Qiaoyang Teng,Xuesong Li,Nancy Beerens,Zejun Li. 2023

[10]A Pool of Ferritin Nanoparticles Delivering Six Proteins of African Swine Fever Virus Induces Robust Humoral and Cellular Immune Responses in Pigs. Lu, Zhanhao,Zhong, Dailang,Song, Xin,Lan, Jing,Wang, Yanjin,Luo, Rui,Chen, Shengmei,Huang, Ruojia,Qiu, Hua-Ji,Li, Yongfeng,Wang, Tao,Sun, Yuan. 2026

[11]CpG基序的作用机理及其在寄生虫学上的应用. 傅志强,刘金明. 2001

[12]CpGDNA对鸡传染性喉气管炎病毒DNA疫苗免疫效果的影响. 孟松树,李曦,智海东,王云峰,王柳,童光志. 2001

[13]CpG模体的免疫激活机制及应用. 窦永喜,景志忠,张玉德. 2004

[14]CpG DNA对鸡传染性喉气管炎病毒DNA疫苗免疫效果的影响. 孟松树,李曦,智海东,王云峰,王柳,童光志. 2001

[15]经分子佐剂诱导产生的卵黄抗体对NDRV的预防和治疗效力评价. 武华,孟利佳,阴雅洁,乔思娜,倪维玲,陈立功,侯绍华,徐倩倩,董世山. 2022

[16]CpG和poly I:C增强口蹄疫病毒样颗粒疫苗免疫效果的研究. 任丽华,罗新,董虎,谭书桢,郭慧琛,孙世琪,魏彦明,滕志东. 2024

[17]Multiple-trait genome-wide association study based on principal component analysis for residual covariance matrix. Gao, H.,Wu, Y.,Li, J.,Zhang, T.,Wu, Y.,Jiang, L.,Zhan, J.,Yang, R..

[18]EgSeverin and Eg14-3-3zeta from Echinococcus granulosus are potential antigens for serological diagnosis of echinococcosis in dogs and sheep. Xiaoli Zhang,Chenxi Wei,Yajie Lv,Rongsheng Mi,Baoping Guo,Sajid Ur Rahman,Yehua Zhang,Long Cheng,Haiyan Jia,Yan Huang,Xiangan Han,Haiyan Gong,Zhaoguo Chen. 2023

[19]A systematic review and meta-analysis on prevalence and distribution of Taenia and Echinococcus infections in Ethiopia. Nigus Abebe Shumuye,John Asekhaen Ohiolei,Mebrahtu Berhe Gebremedhin,Hong Bin Yan,Li Li,Wen Hui Li,Nian Zhang Zhang,Bao Quan Fu,Wan Zhong Jia. 2021

[20]Echinococcus granulosus sensu lato in animal intermediate hosts: What is with the organ location?. John Asekhaen Ohiolei,Hong Bin Yan,Paul Olalekan Odeniran,Li Li,Nigus Abebe Shumuye,Sayed Ajmal Qurishi,Clement Isaac,Bao Quan Fu,Wan Zhong Jia. 2022

作者其他论文 更多>>