数字农科院2.0

Fusion Partner Facilitates Expression of Cell-Penetrating Peptide L2 in Pichia pastoris

文献类型： 外文期刊

作者： Xuan Li;Na Yang;Yuxin Fang;Ruoyu Mao;Ya Hao;Da Teng;Na Dong;Anshan Shan;Jianhua Wang

作者机构：

关键词： cell-penetrating peptide L2;fusion expression;Pichia pastoris;small fusion tags;small ubiquitin-related modifier (SUMO)

期刊名称： Antibiotics

ISSN： 2079-6382

年卷期： 2024 年 13 卷 12 期

页码：

收录情况： SCIE（2024版）

摘要： Background: L2 is formed by combining the pheromone of Streptococcus agalactiae (S. agalactiae) and a cell-penetrating peptide (CPP) with cell-penetrating selectivity. L2 has more significant penetration and better specificity for killing S. agalactiae. However, the production of AMPs by chemical synthesis is always a challenge because of the production cost. Methods: This study was devoted to the heterologous expression of the cell-penetrating peptide L2 in Pichia pastoris using SUMO and a short acidic fusion tag as fusion partners, and the high-density expression of SUMO-L2 was achieved in a 5 L fermenter. Results: The results showed that SUMO-L2 expression in the 5 L fermenter reached 629 mg/L. The antibacterial activity of recombinant L2 was examined; the minimum inhibitory concentration (MICs) and minimum bactericidal concentration (MBCs) of purified L2 were 4–8 μg/mL and 8–16 μg/mL against S. agalactiae after 84 h of lysis with 50% formic acid. Conclusions: The findings suggest that SUMO is a suitable fusion tag to express cell-penetrating peptide L2.

分类号：

  • 相关文献

[1]Fusion strategy enables high-level expression of fungal laccase in Pichia pastoris for efficient detoxification of aflatoxin B1 and zearalenone. Lin Cui,Mengting Liu,Yang He,Ruiju Miao,Huiying Luo,Huoqing Huang,Xiaoyun Su、田健、Tao Tu,Yuan Wang,Xing Qin,Honglian Zhang,Jie Zhang,Yaru Wang,Bin Yao,Yingguo Bai,Xiaolu Wang. 2025

[2]Fusion expression of bovine lactoferricin in Escherichia coli. Feng, Xing-jun,Wang, Jian-hua,Shan, An-shan,Teng, Da,Yang, Ya-lin,Yao, Yi,Yang, Guan-pin,Shao, Yan-chun,Liu, Shuo,Zhang, Fan.

[3]Multimerization and fusion expression of bovine lactoferricin derivative LfcinB15-W4,10 in Escherichia coli. Tian, Zi-gang,Da Teng,Yang, Ya-lin,Luo, Jin,Feng, Xing-jun,Fan, Ying,Zhang, Fan,Wang, Jian-hua.

[4]Decreased Rhamnose Metabolic Flux Improved P.roduction Of Target Proteins A nd Cell Flocculation In Pichia Pastoris. Zhang, Xue,Zhang, Yuwei,Zhang, Yuhong,Liu, Bo,Zhang, Wei,Yan, Chengliang,Xu, Xinxin,Zhang, Zhifang. 2018

[5]Basal Transcription Profiles Of The R.hamnose-Inducible Promoter P-Lra3 And T he Development Of Efficient P-Lra3-Based Systems For Markerless Gene Deletion And A Mutant Library In Pichia Pastoris. Liu, Bo,Xu, Xinxin,Zhang, Wei,Zhang, Yuhong,Liang, Mingli,Jiao, Jian,Wang, Shuai. 2019

[6]Deleting the first disulphide bond in an arenicin derivative enhances its expression in Pichia pastoris. Yang, N.,Wang, X.,Teng, D.,Mao, R.,Hao, Y.,Wang, J.,Yang, N.,Wang, X.,Teng, D.,Mao, R.,Hao, Y.,Wang, J.,Feng, X..

[7]Preparation of high-purity fructo-oligosaccharides by Aspergillus japonicus beta-fructofuranosidase and successive cultivation with yeast. Yang, Ya-Lin,Wang, Jian-Hua,Teng, Da,Zhang, Fan.

[8]Recombinant production of the antimicrobial peptide NZ17074 in Pichia pastoris using SUMO3 as a fusion partner. Wang, X. J.,Wang, X. M.,Teng, D.,Zhang, Y.,Mao, R. Y.,Wang, J. H.,Wang, X. J.,Wang, X. M.,Teng, D.,Zhang, Y.,Mao, R. Y.,Wang, J. H..

[9]Eukaryotic expression and antimicrobial spectrum determination of the peptide tachyplesin II. Xu, Feng,Meng, Kun,Wang, Ya-Ru,Lu, Hui-Ying,Yang, Pei-Long,Yao, Bin,Xu, Feng,Wu, Ning-Feng,Fan, Yun-Liu.

[10]High-level expression and immunogenicity of a porcine circovirus type 2 capsid protein through codon optimization in Pichia pastoris. Tu, Yabin,Wang, Yanqun,Wang, Gang,Wu, Jianan,Liu, Yonggang,Wang, Shujie,Jiang, Chenggang,Cai, Xuehui.

[11]An acidic pectin lyase from Aspergillus niger with favourable efficiency in fruit juice clarification. Xu, S. X.,Qin, X.,Wu, K.,Qin, X.,Liu, B.,Zhang, D. Q.,Zhang, W.,Zhang, Y. H..

[12]Molecular cloning and characterization of a novel alpha-galactosidase gene from Penicillium sp F63 CGMCC 1669 and expression in Pichia pastoris. Mi, Shijun,Meng, Kun,Wang, Yaru,Bai, Yingguo,Yuan, Tiezheng,Luo, Huiying,Yao, Bin. 2007

[13]Expression of serine proteinase P186 of Arthrobotrys oligospora and analysis of its nematode-degrading activity. Zhao, Hailong,Qiao, Jun,Meng, Qingling,Gong, Shasha,Chen, Cheng,Liu, Tianli,Tian, Lulu,Chen, Chuangfu,Cai, Xuepeng,Luo, Jianxun.

[14]Functional expression of the keratinolytic serine protease gene sfp2 from Streptomyces fradiae var. k11 in Pichia pastoris. Li, Jiang,Shi, Peng-Jun,Han, Xiao-Yu,Meng, Kun,Yang, Pei-Long,Wang, Ya-Ru,Luo, Hui-Ying,Wu, Ning-Feng,Yao, Bin,Fan, Yun-Liu.

[15]Expression of tachyplesin gene in yeast Pichia pastoris. Zhang, CY,Zhao, J,Wu, CJ,Fan, YL. 1998

[16]A thermophilic beta-mannanase from Neosartorya fischeri P1 with broad pH stability and significant hydrolysis ability of various mannan polymers. Yang, Hong,Shi, Pengjun,Lu, Haiqiang,Wang, Huimin,Luo, Huiying,Huang, Huoging,Yang, Peilong,Yao, Bin.

[17]Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: Purification and characterization. Chu, Xiao-Yu,Wu, Ning-Feng,Deng, Min-Jie,Tian, Jian,Yao, Bin,Fan, Yun-Liu.

[18]A thermophilic and acid stable family-10 xylanase from the acidophilic fungus Bispora sp MEY-1. Luo, Huiying,Yang, Jun,Wang, Hui,Yang, Yuhui,Huang, Huoqing,Shi, Pengjun,Yuan, Tiezheng,Yao, Bin,Li, Jiang,Fan, Yunliu.

[19]Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33. Mao, Ruoyu,Teng, Da,Wang, Xiumin,Zhang, Yong,Jiao, Jian,Cao, Xintao,Wang, Jianhua,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Zhang, Yong,Jiao, Jian,Cao, Xintao,Wang, Jianhua. 2015

[20]Phytase production by fermentation of recombinant Pichia pastoris in monosodium glutamate wastewater. Bai, Yingguo,Yang, Peilong,Wang, Yaru,Shi, Pengjun,Luo, Huiying,Meng, Kun,Wu, Bo,Yao, Bin.

作者其他论文 更多>>