数字农科院2.0

The microrna mir-14 regulates egg-laying by targeting ecr in honeybees (Apis mellifera)

文献类型： 外文期刊

作者： Chen Xiao;Fu Jinluan

作者机构：

关键词： EcR; Egg number; Honeybees; MiRNA

期刊名称： Insects

ISSN： 2075-4450

年卷期： 2021 年 12 卷 4 期

页码：

收录情况： JCR（2021版）

摘要： Honeybees (Apis mellifera) are important pollinators and are commonly used for honey production. The oviposition behavior in honeybees is complex and errors in oviposition could affect the development of the bee colony. Recent studies reported that RNA–RNA cross-talk played a critical role in several biological processes, including reproduction. Ecdysone receptor (EcR) and miR-14 were previously reported to play important roles in egg-laying. Moreover, EcR was predicted to be the target gene of miR-14 and may form miR-14-EcR cross-talk. In this study, knocking down and overexpression of miR-14 and EcR in queen model were implemented. The effect of RNA expression of miR-14 and EcR on the number of eggs laid by honeybee queens were analyzed. Further, luciferase assay was used to confirm the target relation between miR-14 and 3′ UTR of EcR. The results showed that the expression of miR-14 and EcR was associated with the number of eggs laid by queens. In specific, inhibition of miR-14 expression enhanced the number of eggs laid, while overexpression of EcR enhanced the number of eggs laid. Lastly, we determined that miR-14 directly targets the mRNA of EcR. These findings suggest that the cross-talk of miR-14-EcR plays an important role in the number of eggs laid by honeybee queens. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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