数字农科院2.0

Identifying long non-coding RNAs and characterizing their functional roles in swine mammary gland from colostrogenesis to lactogenesis br

文献类型： 外文期刊

作者： Shi, Lijun;Zhang, Longchao;Wang, Ligang;Liu, Xin;Gao, Hongmei;Hou, Xinhua;Zhao, Fuping;Yan, Hua;Cai, Wentao;Wang, Lixian

作者机构：

关键词： Colostrum;LncRNA;Mammary Gland;Pig;Weighted Gene Co-expression Network Analysis (WGCNA)

期刊名称： ANIMAL BIOSCIENCE

ISSN： 2765-0189

年卷期： 2022 年 35 卷 6 期

页码：

收录情况： SCIE（2022版）

摘要： Objective: This study was conducted to identify the functional long non-coding RNAs (lncRNAs) for swine lactation by RNA-seq data of mammary gland.Methods: According to the RNA-seq data of swine mammary gland, we screened lncRNAs, performed differential expression analysis, and confirmed the functional lncRNAs for swine lactation by validation of genome wide association study (GWAS) signals, functional anno-tation and weighted gene co-expression network analysis (WGCNA).Results: We totally identified 286 differentially expressed (DE) lncRNAs in mammary gland at different stages from 14 days prior to (-) parturition to day 1 after (+) parturition, and the expressions of most of lncRNAs were strongly changed from day -2 to day +1. Further, the GWAS signals of sow milk ability trait were significantly enriched in DE lncRNAs. Functional annotation revealed that these DE lncRNAs were mainly involved in mammary gland and lactation developing, milk composition metabolism and colostrum function. By performing weighted WGCNA, we identified 7 out of 12 lncRNA-mRNA modules that were highly associated with the mammary gland at day -14, day -2, and day +1, in which, 35 lncRNAs and 319 mRNAs were involved.Conclusion: This study suggested that 18 lncRNAs and their 20 target genes were promising candidates for swine parturition and colostrum occurrence processes. Our research provided new insights into lncRNA profiles and their regulating mechanisms from colostrogenesis to lactogenesis in swine

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