数字农科院2.0

Identification fi cation of an Aux/IAA regulator for fl esh fi rmness using combined GWAS and bulked segregant RNA-Seq analysis in watermelon

文献类型： 外文期刊

作者： Anees, Muhammad;Zhu, Hongju;Umer, Muhammad Jawad;Gong, Chengsheng;Yuan, Pingli;Lu, Xuqiang;He, Nan;Kaseb, Mohamed Omar;Yang, Dongdong;Zhao, Yong;Liu, Wenge

作者机构：

关键词： Watermelon;Flesh firmness;GWAS;BSR-Seq;KASP marker analysis;Transient overexpression

期刊名称： HORTICULTURAL PLANT JOURNAL

ISSN： 2095-9885

年卷期： 2024 年 10 卷 5 期

页码：

收录情况： SCIE（2024版） ; ; CSCD（2023-2024年度） ; ; 科技核心（2024版） ; ; 农林核心（2020版）

摘要： Watermelon is a highly cultivated fruit crop renowned for its quality properties of fruit flesh. Among various quality factors, fruit flesh firmness is a crucial quality parameter influencing the fruit texture, shelf life and its commercial value. The auxin/indole-3-acetic acid (Aux/ IAA) plays a significant role in fruit development and ripening of non-climacteric fruits. However, the regulatory mechanism of Aux/IAA in controlling fruit flesh firmness and ripening in watermelon remains unknown. In this study, we employed an integrative approach combining genome-wide association study (GWAS) and bulked segregant RNA-Seq analysis (BSR-Seq) to identify an overlapping candidate region between 12 776 310 and 12 968 331 bp on chromosome 6, underlying an auxin-responsive gene (Aux/IAA) associated with flesh firmness in watermelon. Transcriptome analysis, followed by real-time quantitative reverse transcription PCR (qRT-PCR), confirmed that the expression of Aux/IAA was consistently higher in fruits with high flesh firmness. The sequence alignment revealed a single base mutation in the coding region of Aux/IAA. Furthermore, the concomitant Kompetitive/Competitive allele-specific PCR (KASP) genotyping data sets for F2 2 population and germplasm accessions identified Aux/IAA as a strong candidate gene associated with flesh firmness. Aux/IAA was enriched in the plant hormone signal transduction pathway, involving cell enlargement and leading to low flesh firmness. We determined the higher accumulation of abscisic acid (ABA) in fruits with low flesh firmness than hard flesh. Moreover, overexpression of Aux/IAA induced higher flesh firmness with an increased number of fruit flesh cells while reducing ABA content and flesh cell sizes. Additionally, the allelic variation in Aux/IAA for soft flesh firmness was found to exist in Citrullus mucosospermus and gradually fixed into Citrullus lanatus during domestication, indicating that soft flesh firmness was a domesticated trait. These findings significantly enhanced our understanding of watermelon fruit flesh firmness and consequently the watermelon fruit quality.

分类号：

  • 相关文献

[1]Identification of Key Gene Networks Associated With Cell Wall Components Leading to Flesh Firmness in Watermelon. Muhammad Anees,Lei Gao,Muhammad Jawad Umer,Pingli Yuan,Hongju Zhu,Xuqiang Lu,Nan He,Chengsheng Gong,Mohamed Omar Kaseb,Shengjie Zhao,Wenge Liu. 2021

[2]Genome-wide association study provides genetic insights into natural variation in watermelon rind thickness and single fruit weight. Chengsheng Gong,Xuqiang Lu,Hongju Zhu,Muhammad Anees,Nan He,Wenge Liu. 2022

[3]A 48 kb fragment deletion is required for the formation of trichomes in watermelon. Yu Wang,Na Li,Nannan Li,Jianli Shang,Dan Zhou,Shuangwu Ma. 2025

[4]利用BSR-Seq定位小麦品种郑麦103抗条锈病基因YrZM103. 张怀志,谢菁忠,陈永兴,刘旭,王勇,闫素红,杨兆生,赵虹,王西成,贾联合,曹廷杰,刘志勇. 2017

[5]小麦农家品种武都白茧儿抗条锈病基因定位. 董亚超,董艳,赵聪,李雷,商航,肖永贵,白斌,夏先春,刘金栋,耿洪伟. 2024

[6]西瓜EST资源的SSR信息分析及标记开发. 赵胜杰,刘文革,阎志红,何楠,包文风. 2009

[7]施用钾肥对西瓜番茄红素含量的影响. 何楠,赵胜杰,刘文革,阎志红,万学闪. 2009

[8]NaCl胁迫对不同西瓜种质发芽的影响. 阎志红,刘文革,赵胜杰,何楠,王俊良. 2009

[9]不同倍性西瓜果实Vc含量比较研究. 程志强,刘文革,刘志敏,阎志红,赵胜杰,何楠,张俊杰. 2009

[10]不同染色体倍性西瓜花粉形态观察比较. 刘文革,王鸣,阎志红. 2009

[11]蛋鸡资源群体的盲肠长度全基因组关联分析. 王星果,曲亮,卢建,窦套存,马猛,胡玉萍,沈曼曼,郭军,李尚民,王克华. 2018

[12]中国籼稻群体的遗传结构解析及12个株型性状的全基因组关联分析. 鲁清,章孟臣,魏兴华. 2015

[13]北京油鸡血清IgG含量的GWAS初步分析. 张磊,刘冉冉,赵桂苹,文杰,郑麦青,吴丹,胡耀东,李鹏,刘丽. 2011

[14]黄瓜发芽期耐热性评价及全基因组关联分析. 张松,苗晗,宋子超,薄凯亮,王烨,顾兴芳,张圣平. 2019

[15]功能基因筛选方法的研究进展. 姚雅馨,喇永富,汤继顺,贺小云,张壮彪,储明星. 2020

[16]玉米叶片叶绿素含量的全基因组关联性分析. 滕守振1,,2 汪海2 梁海生1,,2 辛红佳2 李圣彦2 郎志宏2. 2017

[17]基于通路分析方法诠释肉鸡体重性状全基因组关联研究. 王杰,刘璐,刘杰,赵桂苹,刘冉冉,郑麦青,文杰. 2017

[18]黄瓜核心种质幼苗耐低温性评价及GWAS分析. 王伟平,宋子超,薄凯亮,董邵云,魏爽,苗晗,李锦斌,张圣平,顾兴芳. 2019

[19]黄瓜核心种质幼苗耐热性评价及GWAS分析. 魏爽,张松,薄凯亮,王伟平,苗晗,董邵云,顾兴芳,张圣平. 2019

[20]利用两种统计模型对中国肉用西门塔尔牛屠宰性状的全基因组关联分析. 常天鹏,夏江威,宝金山,金生云,朱波,徐凌洋,陈燕,张路培,高雪,李俊雅,高会江. 2018

作者其他论文 更多>>