数字农科院2.0

Prime editing‐mediated correction of the endogenousxa23 gene confers broad‐spectrum resistance to ricebacterial blight

文献类型： 外文期刊

作者： Man Yu;; Sujie Zhang;;Junlian Yan;;Jinguang Huang;;Zhiyuan Ji;;Xueping Zhou;; Wenxian Sun;;Huanbin Zhou

关键词： bacterial blight resistance; CRISPR/Cas; Oryza sativa L.; prime editing; Xa23

期刊名称： New Plant Protection

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年卷期： 2025 年

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收录情况： 无来源刊（2025版）

摘要： Rice bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), isa devastating disease that threatens global rice (Oryza sativa L.) production.Xoo delivers transcription activator‐like effectors that bind to effector bindingelements in the promoters of host susceptibility (S) or resistance (R) genes,thereby triggering either disease development or defense responses. Xa23 is abroad‐spectrum R gene that confers strong BB resistance by recognizing theconserved EBEAvrXa23 in its promoter. In this study, we used an ScCas9‐basedprime editing system to precisely correct three consecutive mutations in thedysfunctional EBEAvrXa23 sequence of the endogenous xa23 promoter in thesusceptible japonica cultivar N9208. The engineered N9208(Xa23) linerestored Xa23 inducibility upon Xoo infection and exhibited robust resistanceagainst diverse Xoo strains without growth penalties. These results demon-strate a universal, precise, efffcient, and biosafe strategy for engineeringendogenous executor R genes and rapidly upgrading commercial rice cultivarsfor BB resistance without introducing exogenous DNA.

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