数字农科院2.0

Development and Application of an In Vitro Pollen Viability Assay for Comparative Safety Assessment of Transgenic Alfalfa (Medicago sativa L.)

文献类型: 外文期刊

作者: Chen, Yuxiao;Zhang, Xiaochun;Yang, Jiangtao;Guo, Diandian;Wang, Xujing;Wang, Zhixing

作者机构:

关键词: pollen viability;biosafety;alfalfa;protocol

期刊名称: PLANTS-BASEL

ISSN: 2223-7747

年卷期: 2025 年 14 卷 19 期

页码:

收录情况: SCIE(2025版)

摘要: Alfalfa (Medicago sativa L.) is a vital global forage crop. Transgenic technology promises enhanced yield and quality, but requires rigorous environmental risk assessment, particularly regarding pollen-mediated gene flow, for which standardized protocols are lacking. Based on an optimized in vitro culture medium, this study developed a method to assess alfalfa pollen viability. Using a single-factor experimental design, key assessment parameters were established at 1/4/8 h and 20/30/40 degrees C. A comparative analysis revealed no significant difference (p > 0.05) in pollen viability between the transgenic line SA6-8 and its non-transgenic parent ZM-1 within this evaluation system. This result indicates that the genetic modification did not impact the pollen viability of SA6-8. By establishing this in vitro germination-based pollen viability assessment system and comparatively analyzing pollen viability between transgenic alfalfa and its non-transgenic parent under diverse environmental conditions, our approach provides crucial insights for optimizing transgenic alfalfa planting strategies and strengthening biosafety review protocols.

分类号:

  • 相关文献

[1]卫星搭载对紫花苜蓿当代植株生物量的影响(英文). 任卫波,赵亮,王蜜,陈立波,郭慧琴. 2008

[2]An optimized protocol for detecting guard cell specific gene expression by in situ RT-PCR in Brassica rapa. Yingying Song,Xinlei Guo,Jian Wu,Jianli Liang,Runmao Lin,Zifu Yan,Xiaowu Wang. 2022

[3]GLUCAN SYNTHASE-LIKE 5 (GSL5) Plays an Essential Role in Male Fertility by Regulating Callose Metabolism During Microsporogenesis in Rice. Shi, Xiao,Sun, Xuehui,Zhang, Zhiguo,Feng, Dan,Zhang, Qian,Han, Lida,Wu, Jinxia,Lu, Tiegang.

[4]Pollen Semi-Sterility1 Encodes a Kinesin-1-Like Protein Important for Male Meiosis, Anther Dehiscence, and Fertility in Rice. Zhou, Shirong,Wang, Yang,Li, Wanchang,Zhao, Zhigang,Ren, Yulong,Wang, Yong,Jiang, Ling,Liu, Linglong,Wan, Jianmin,Zhou, Shirong,Gu, Suhai,Lin, Qibing,Wang, Dan,Su, Ning,Zhang, Xin,Cheng, Zhijun,Lei, Cailin,Wang, Jiulin,Guo, Xiuping,Wu, Fuqing,Wang, Haiyang,Wan, Jianmin,Ikehashi, Hiroshi.

[5]Pollen viability and stigma receptivity in Lilium during anthesis. He, Guoren,Ming, Jun,Liu, Chun,Yuan, Suxia,He, Guoren,Hu, Fengrong.

[6]Shade Delayed Flowering Phenology and Decreased Reproductive Growth of Medicago sativa L.. Qin, Fengfei,Shen, Yixin,Li, Zhihua,Qu, Hui,Feng, Jinxia,Kong, Lingna,Teri, Gele,Luan, Haoming,Cao, Zhiling. 2022

[7]Cloning and Characterization of Protein Prenyltransferase Alpha Subunit in Rice. Wang Tao,Lou Lijuan,Li Zeyu,Shang Lianguang,Wang Quan. 2021

[8]Pollen grain number and viability contribute to variation in effective ovule number among oilseed rape genotypes. Xiao Guo,Bao Luo Ma,Neil B. McLaughlin,Yajun Gao,Xiaoming Wu,Biyun Chen. 2022

[9]A Novel Laboratory Protocol for Pollen Viability Assessment to Inform Biosafety Evaluation of Transgenic Rice (Oryza sativa L.). Yuxiao Chen,Caiyue Liu,Xiaochun Zhang,Yufeng Dong,Jiangtao Yang,Dongmei Wang,Zhixing Wang,Xujing Wang. 2025

[10]A Novel Laboratory Protocol for Pollen Viability Assessment to Inform Biosafety Evaluation of Transgenic Rice. 陈宇枭,,刘彩月,,张晓春,,董玉凤,,王冬梅,,杨江涛,,王志兴,,王旭静. 2025

[11]Optimization of grape artificial hybrid pollination technology process and the effect of different male parents on the fruit setting rate. Zhou, Ruijin,Gai, Ruxin,Liao, Wanying,Wu, Qiujie,Cheng, Jiahui,Li, Yongmu,Fan, Xiucai,Li, Guirong. 2025

[12]Response Of The Bacterial Community O.f Propylea Japonica (Thunberg) T o Cry2Ab Protein. Cui, Jinjie,Zhang, Shuai,Zhang, Shuai,Ma, Yan,Luo, Junyu,Wu, Linke,Zhang, Lijuan,Wang, Li,Ji, Jichao,Cui, Jinjie,Jiang, Weili. 2019

[13]GM wheat development in China: current status and challenges to commercialization. Jones, Huw D.,Xia, Lanqin,Ma, Youzhi,He, Yi. 2012

[14]Genetic diversity and gene flow dynamics revealed in the rare mixed populations of wild soybean (Glycine soja) and semi-wild type (Glycine gracilis) in China. Wang, Ke-Jing,Li, Xiang-Hua. 2013

[15]Early instar response to plant-delivered Bt-toxin in a herbivore (Spodoptera litura) and a predator (Propylaea japonica). Zhang, GF,Wan, FH,Liu, WX,Guo, HY. 2006

[16]A 90-day safety study of genetically modified rice expressing rhIGF-1 protein in C57BL/6J rats. Tang, Maoxue,Cheng, Wenke,Qian, Lili,Yang, Shulin,Cui, Wentao,Li, Kui,Tang, Maoxue,Cheng, Wenke,Qian, Lili,Yang, Shulin,Cui, Wentao,Li, Kui,Xie, Tingting,Yang, Daichang.

[17]Safety assessment of sFat-1 transgenic pigs by detecting their co-habitant microbe in intestinal tract. Tang, Maoxue,Cheng, Wenke,Jin, Erhui,Yang, Shulin,Cui, Wentao,Li, Kui,Tang, Maoxue,Cheng, Wenke,Jin, Erhui,Yang, Shulin,Cui, Wentao,Li, Kui,Zheng, Xinmin,Chen, Hongxing.

[18]Measuring gene flow in the cultivation of transgenic cotton (Gossypium hirsutum L.). Bao-Hong Zhang,Xiao-Ping Pan,Teng-Long Guo,Qing-Lian Wang,Todd A. Anderson. 2005

[19]Brazil and the development of international scientific biosafety testing guidelines for transgenic crops. Capalbo, DMF,Hilbeck, A,Andow, D,Snow, A,Bong, BB,Wan, FH,Fontes, EMG,Osir, EO,Fitt, GP,Johnston, J,Songa, J,Heong, KL,Birch, ANE. 2003

[20]Advances in the biosafety research of non-target DNA in transgenic plants. Ma, RC,Peng, YF,Guan, HS. 2001

作者其他论文 更多>>