数字农科院2.0

PMT family mannosyltransferase-mediated protein O-mannosylation is required for the development and pathogenicity of Colletotrichum graminicola

文献类型： 外文期刊

作者： Jie Mei;Na Ning;Yan Yang;Shaoqun Zhou;Wende Liu

作者机构：

关键词： Colletotrichum graminicola;Maize anthracnose;O-mannosylation;PMT family protein

期刊名称： Phytopathology Research

ISSN： 2096-5362

年卷期： 2025 年 7 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; 农林核心（2024版）

摘要： Protein O-mannosylation in the endoplasmic reticulum (ER) plays a crucial regulatory role in the development and virulence of many phytopathogenic fungi. However, the corresponding biological function and underlying regulatory mechanism mediated by this process in Colletotrichum graminicola, causing the anthracnose disease of maize (Zea mays), is unclear. The protein O-mannosyltransferase (PMT) family-mediated protein O-mannosylation is absent in plants, which may provide good targets for developing safe antifungal agents. In the present study, we identified three PMT family mannosyltransferases: CgPMT1, CgPMT2, and CgPMT4 in C. graminicola. The subcellular localization analyses proved that these proteins were located in the ER as previously reported. qRT-PCR results showed that the expression levels of all the three genes were highest in the conidia compared with other developmental stages. Gene knockout analysis demonstrated that the PMT-mediated protein O-mannosylation was essential for the vegetative growth, conidiation, appressorium formation, and pathogenicity of C. graminicola. Immunoprecipitation-Mass Spectrometry (IP-MS) analysis showed that the potential interaction proteins of these CgPMTs were mainly enriched in the pathways of sphingolipid metabolism, autophagy, glycosylphosphatidylinositol (GPI)-anchor modification, and endocytosis. These findings enhanced our understanding of the functions of protein O-mannosylation and provide effective molecular targets for maize disease prevention and control.

分类号：

  • 相关文献

[1]Genome Sequence Resource of a Colletotrichum graminicola Field Strain from China. Shi, Xuetao,Xia, Xinyao,Mei, Jie,Gong, Ziwen,Zhang, Junqi,Xiao, Yutao,Duan, Canxing,Liu, Wende. 2023

[2]Effector secretion and stability in the maize anthracnose pathogen Colletotrichum graminicola requires N-linked protein glycosylation and the ER chaperone pathway. Mei, Jie,Li, Zhiqiang,Zhou, Shaoqun,Chen, Xiao-Lin,Wilson, Richard A.,Liu, Wende. 2023

[3]Genome-Wide Identification and Analysis of Carbohydrate-Binding Modules in Colletotrichum graminicola. Ya Fei Wang,Qiu Li Huang,Xin Yu Chen,Hong Lian Li,Jia Xin Chang,Yu Zhang,Yi Wen Wang,Yan Shi. 2025

[4]The Candidate Effector Cgmas2 Orchestrates Biphasic Infection of Colletotrichum graminicola in Maize by Coordinating Invasive Growth and Suppressing Host Immunity. Gong, Ziwen,Yao, Jinai,Ma, Yuqing,Xia, Xinyao,Zhang, Kai,Mei, Jie,Sun, Tongjun,Wang, Yafei,Li, Zhiqiang. 2026

作者其他论文 更多>>