数字农科院2.0

Efficient Virus-Induced Gene Silencing (VIGS) Method for Discovery of Resistance Genes in Soybean

文献类型： 外文期刊

作者： Deng, Kelin;Lu, Zihua;Yang, Hongli;Chen, Shuilian;Li, Chao;Cao, Dong;Wang, Hongwei;Hao, Qingnan;Chen, Haifeng;Shan, Zhihui

作者机构：

关键词： soybean;TRV-VIGS;GmPDS;gene screening;functional validation

期刊名称： PLANTS-BASEL

ISSN： 2223-7747

年卷期： 2025 年 14 卷 10 期

页码：

收录情况： SCIE（2025版）

摘要： Soybean (Glycine max L.) is a vital grain and oil crop, serving as a primary source of edible oil, plant-based protein, and livestock feed. Its production is crucial for ensuring global food security. However, soybean yields are severely impacted by various diseases, and the development of disease-resistant cultivars remains the most sustainable strategy for mitigating these losses. While stable genetic transformation is a common approach for studying gene function, virus-induced gene silencing (VIGS) offers a rapid and powerful alternative for functional genomics, enabling efficient screening of candidate genes. Nevertheless, the application of VIGS in soybean has been relatively limited. In this study, we established a tobacco rattle virus (TRV)-based VIGS system for soybean, utilizing Agrobacterium tumefaciens-mediated infection. The TRV vector was delivered through cotyledon nodes, facilitating systemic spread and effective silencing of endogenous genes. Our results demonstrate that this TRV-VIGS system efficiently silences target genes in soybean, inducing significant phenotypic changes with a silencing efficiency ranging from 65% to 95%. Key genes, including phytoene desaturase (GmPDS), the rust resistance gene GmRpp6907, and the defense-related gene GmRPT4, were successfully silenced, confirming the system's robustness. This work establishes a highly efficient TRV-VIGS platform for rapid gene function validation in soybean, providing a valuable tool for future genetic and disease resistance research.

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