数字农科院2.0

Metagenomics insights into the functional profiles of soil carbon, nitrogen under long-term chemical and humic acid urea application

文献类型： 外文期刊

作者： Tengfei Guo 1 ; Mengyuan Wang; Ke Yue; Peng Luo; Xiao Song ; Shaomin Huang; Xinpeng Xu ; Qian Zhang; Yulu Chen; Zekun Zhang; Sumiao Su; Shijie Ding; Shuiqing Zhang; Kun Zhou

关键词： metagenomics; carbon cycling; nitrogen cycling; humic acid urea

期刊名称： Agronomy-Basel

ISSN：

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Long-term positioning tests can systematically reveal the evolution characteristics of soil fertility and crop productivity, and reflect the spatiotemporal changes in soil quality and their driving factors. While soil microorganisms mediating nutrient cycling are crucial for maintaining crop productivity and the long-term resilience of agricultural ecosystems, how prolonged use of different fertilization strategies affects their functional capacity remains insufficiently understood. In this study, we applied metagenomic sequencing to investigate how three fertilization treatments, namely (i) N0 receiving only phosphorus (P) and potassium (K) fertilizers, (ii) N250 receiving conventional urea + P and K, and (iii) F250 receiving humic acid urea + P and K, influence soil microbial communities, functional genes related to C and N cycling, and associated soil properties in a long-term field experiment. The F250 treatment significantly increased average annual yields of wheat and maize to 7166.21 kg hm−2 and 8309.96 kg hm−2, respectively. These values were 148.66% and 73.47% higher than those under N0, and 8.22% and 11.64% higher than those under N250. Compared with N0, both N250 and F250 signally augmented soil nitrate, ammonium, total nitrogen (TN), and soil organic carbon (SOC), altered microbial community composition, and enhanced the relative abundance of genes engaged in C fixation and methane oxidation. Both treatments also promoted denitrification and dissimilatory nitrate reduction to ammonium (DNRA). Relative to N250, F250 specifically enriched the beneficial bacterial genus Pedobacter, further increased the abundance of the C fixation gene pccA, and markedly upregulated the DNRA gene nrfA. Soil TN and SOC were identified as the key environmental factors regulating microbial community structure and the functional potential of C and N cycling pathways. Collectively, our findings provide a mechanistic understanding of how long-term application of humic acid urea enhances crop productivity by modulating the genetic potential of soil microorganisms in biogeochemical cycles, offering a biological foundation for optimizing fertilization strategies in sustainable agriculture.

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