数字农科院2.0

TaTPS11 enhances wheat cold resistance by regulating source-sink factor

文献类型： 外文期刊

作者： Xiaoguang Lu;Fuzhi Zhang;Chenglong Zhang;Guorui Li;Yuchen Du;Cicong Zhao;Wei Zhao;Fengmei Gao;Lianshuang Fu;Xin Liu;Jun Liu;Xiaonan Wang

作者机构：

关键词： CRISPR/Cas9;Sucrose;TaTPS11;Tolerance cold;Trehalose;Wheat

期刊名称： Plant Physiology and Biochemistry

ISSN： 0981-9428

年卷期： 2024 年 211 卷

页码：

收录情况： SCIE（2024版）

摘要： The presence of sugar in plant tissue can lead to an increase in the osmotic pressure within cells, a decrease in the freezing point of plants, and protection against ice crystal damage to the tissue. Trehalose is closely related to sucrose, which comprises the largest proportion of sugar and has become a hot topic of research in recent years. Our previous studies have confirmed that a key trehalose synthesis gene, TaTPS11, from the cold-resistant winter wheat DM1, could enhance the cold resistance of plants by increasing sugar content. However, the underlying mechanism behind this phenomenon remains unclear. In this study, we cloned TaTPS11-6D, edited TaTPS11-6D using CRISPR/Cas9 technology and transformed ‘Fielder’ to obtain T2 generation plants. We screened out OE3-3 and OE8-7 lines with significantly higher cold resistance than that of ‘Fielder’ and Cri 4–3 edited lines with significantly lower cold resistance than that of ‘Fielder’. Low temperature storage limiting factors were measured for OE3-3, OE8-7 and Cri 4–3 treated at different temperatures.The results showed that TaTPS11-6D significantly increased the content of sugar in plants and the transfer of sugar from source to storage organs under cold conditions. The TaTPS11-6D significantly increased the levels of salicylic, jasmonic, and abscisic acids while also significantly decreasing the level of gibberellic acid. Our research improves the model of low temperature storage capacity limiting factor.

分类号：

  • 相关文献

[1]充分发掘保护利用生物多样性;促进农业可持续发展——《生物多样性与小麦改良》简评. 李祥洲. 1997

[2]30个小麦新品系抗条锈病基因分析及成株期抗病性评价. 冯晶,蔺瑞明,林凤,徐世昌. 2013

[3]小麦抗菌蛋白编码基因BS108的克隆及其转基因抗病小麦的创制. 李钊,周淼平,张增艳,庄洪涛,徐惠君,杜丽璞,叶兴国. 2010

[4]渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究(英文). 孙朝霞,侯思宇,王玉国. 2008

[5]普通小麦品种辐射敏感性模糊聚类分析<英文>. 冯志杰,王琳清. 1992

[6]基因枪转化小麦主要轰击参数的优化. 闵东红,何莎,张彦,夏兰琴. 2013

[7]小麦矮缩病毒分子群体遗传结构研究. 刘艳,王锡锋,周广和. 2008

[8]Induction of mucosal immune responses and protection of cattle against direct-contact challenge by intranasal delivery with foot-and-mouth disease virus antigen mediated by nanoparticles. Pan, Li,Zhang, Zhongwang,Lv, Jianliang,Zhou, Peng,Hu, Wenfa,Fang, Yuzhen,Chen, Haotai,Liu, Xinsheng,Shao, Junjun,Zhao, Furong,Ding, Yaozhong,Lin, Tong,Chang, Huiyun,Zhang, Jie,Zhang, Yongguang,Wang, Yonglu,Zhang, Zhongwang,Lv, Jianliang,Zhou, Peng,Hu, Wenfa,Fang, Yuzhen,Chen, Haotai,Liu, Xinsheng,Shao, Junjun,Zhao, Furong,Ding, Yaozhong,Lin, Tong,Chang, Huiyun,Zhang, Jie,Zhang, Yongguang,Wang, Yonglu. 2014

[9]Cloning, expression and characterization of a trehalose synthase gene from rhodococcus opacus. Yan, Junyan,Qiao, Yu,Ding, Hongbiao,Hu, Jun,Ding, Hongbiao.

[10]Gene cloning, expression, and characterization of a novel trehalose synthase from Arthrobacter aurescens. Wu Xiuli,Ding Hongbiao,Yue Ming,Qiao Yu.

[11]Identification of TPS and TPP gene families in Cannabis sativa and their expression under abiotic stresses. Sun, J.,Dai, Z. G.,Zhang, X. Y.,Tang, Q.,Cheng, C. H.,Liu, C.,Yu, Y.,Xu, G. C.,Xie, D. W.,Su, J. G.. 2022

[12]Transcriptomic and Metabolomic Analysis of the Effects of Exogenous Trehalose on Salt Tolerance in Watermelon (Citrullus lanatus). Yuan, Gaopeng,Sun, Dexi,An, Guolin,Li, Weihua,Si, Wenjing,Liu, Junpu,Zhu, Yingchun. 2022

[13]Trehalose Induced By Reactive Oxygen S.pecies Relieved The Radial G rowth Defects Of Pleurotus Ostreatus Under Heat Stress. Lei, M, Wu, XL, Huang, CY, Qiu, ZH, Wang, LN, Zhang, RY, Zhang, JX. 2019

[14]ABF1 Positively Regulates Rice Chilling Tolerance via Inducing Trehalose Biosynthesis. Yazhou Shu,Wensheng Zhang,Liqun Tang,Zhiyong Li,Xinyong Liu,Xixi Liu,Wanning Liu,Guanghao Li,Jiezheng Ying,Jie Huang,Xiaohong Tong,Honghong Hu,Jian Zhang,Yifeng Wang. 2023

[15]Combined transcriptomics and metabolomics analysis reveals the molecular mechanism of heat tolerance of Le023M, a mutant in Lentinula edodes. Qin Zhang,Rencai Feng,Renyun Miao,Junbin Lin,Luping Cao,Yanqing Ni,Wensheng Li,Xu Zhao. 2023

[16]Insight into the effect of osmosis agents on macro- and micro- texture, water distribution, and thermal stability of instant controlled pressure drop drying peach chips. Wang F.,Bi J.,Lyu M.,Lyu J.. 2024

[17]Textural formation of instant controlled pressure drop–dried peach chips: Investigation of the electrical, thermal, and textural properties of predried peach slices with osmotic dehydration pretreatment. Wang F.,Bi J.,Wang J.,Lyu J.. 2024

[18]Revealing the interaction mechanism between pectin and sugar: A case study of peach pectin and trehalose. Fengzhao Wang,Jinfeng Bi,Yuting Huang,Jian Lyu. 2025

[19]Osmotic dehydration with different agents induced pectin diversity: Physicochemical and structural properties of pectin from instant controlled pressure drop dried peach chips. Wang, Fengzhao,Bi, Jinfeng,Xie, Jin,Wang, Jingxuan,Lyu, Jian. 2025

[20]Trehalose accumulation contributes to enhanced cold stress tolerance in Telenomus remus, a dominant egg parasitoid of Spodoptera frugiperda. Wanbin Chen,Huan Liu,Yuyan Li,Mengqing Wang,Jianjun Mao,Zhijie Guo,Lisheng Zhang. 2025

作者其他论文 更多>>