数字农科院2.0

Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response to Verticillium dahliae and abiotic stress

文献类型： 外文期刊

作者： Xin Zhou;Ling Su;Rui Tang;Yuxuan Dong;Fei Wang;Rong Li;Quanliang Xie;Xianliang Zhang;Guanghui Xiao;Hongbin Li

作者机构：

关键词： abiotic stress response;cotton;HSP40 gene family;HSP70 gene family;Verticillium wilt resistance

期刊名称： Frontiers in Genetics

ISSN： 1664-8021

年卷期： 2023 年 14 卷

页码：

收录情况： SCIE（2023版）

摘要： Introduction: Cotton is an important economic crop to provide natural fibers as raw materials to textile industry, and is significantly affected by biotic and abiotic stress during the whole growth stage, in which Verticillium wilt (VW) caused by Verticillium dahliae is one of the most destructive disease to lead to a significant yield reduction. Heat shock proteins (Hsps) are important molecular chaperones, and play crucial roles in plant growth, development, resistance to biotic and abiotic stress. Hsp40 and Hsp70 are two key Hsps in cell chaperone network, however, the function and regulatory mechanism of Hsp40 and Hsp70 members in VW resistance and abiotic stress in cotton are largely unknown. Methods and Results: Herein, a systematic and comprehensive analysis of Hsp40s and Hsp70s in four cotton species of Gossypium arboretum, G. raimondii, G. hirsutum, and G. barbadense were performed. A total of 291 Hsp40s and 171 Hsp70s identified in four Gossypium species. Sequence analysis revealed that all Hsp40 proteins contained J domain that provides the binding sites to Hsp70. Protein-protein interaction prediction analysis displayed that GhHsp40-55 might interact with GhHsp70-2 and GhHsp70-13, suggesting their potential function as protein complex. Promoter cis-acting element analysis demonstrated that multiple cis-elements related to disease and stress response consists in GhHsp40 and GhHsp70 promoters. Further expression analysis showed that eight GhHsp40s (Hsp40-2,4,8,11,20,23,53,55) and seven GhHsp70s (Hsp70-2,3,6,8,13,19,22) were up-regulated after V. dahliae infection. In addition, five GhHsp40s (Hsp40-2,8,11,53,55) and four GhHsp70s (Hsp70-3,6,8,13) were up-regulated after salt treatment, six GhHsp40s (Hsp40-4,11,20,23) and three GhHsp70s (Hsp70-2,8,19) were up-regulated after drought treatment, four GhHsp40s (Hsp40-2,11,20,23) and four GhHsp70s (Hsp70-3,6,19,22) were up-regulated after temperature treatment, suggesting these Hsps have possible important function in the process of abiotic stress response. Discussion: Our results lay a foundation for understanding the function of Hsp40 and Hsp70 in the resistance against V. dahliae and abiotic stress, and elucidating the regulatory mechanism of the protein complex, evolution and molecular mechanism under stress.

分类号：

  • 相关文献

[1]Genome-wide identification and analysis of a cotton secretome reveals its role in resistance against Verticillium dahliae. Ran Li,Xi Yue Ma,Ye Jing Zhang,Yong Jun Zhang,He Zhu,Sheng Nan Shao,Dan Dan Zhang,Steven J. Klosterman,Xiao Feng Dai,Krishna V. Subbarao,Jie Yin Chen. 2023

[2]Cloning and Expression of the Chloroplast Copper/Zinc-Superoxide Dismutase Gene in Upland Cotton （Gossypium hirsutum L.）. Yu Shuxun,Fan Shuli,Song Meizhen. 2007

[3]2015年全国棉花种植品种监测报告——播种品种(系)344个;数量减少83个;减幅19.4%. 毛树春,冯璐,芦建华. 2016

[4]2015年中国与美国棉花种植品种比较. 冯璐,毛树春. 2016

[5]兰州百合病毒病原的DAS-ELISA检测. 王发林,古勤生,刘芬,彭斌,刘丽锋. 2003

[6]Cloning and characterization of the Gossypium hirsutum major latex protein gene and functional analysis in Arabidopsis thaliana. Chen, Jie-Yin,Dai, Xiao-Feng.

[7]Construction of a bacterial artificial chromosome library for Gossypium herbaceum var. africanum. Gao HaiYan,WANG XingFen,LIU Fang,PENG RenHai,ZHANG Yan,CHENG Hua,WANG KunBo. 2013

[8]Construction and characterization of a bacterial artificial chromosome library for the allotetraploid Gossypium tomentosum. F. Liu,Y.H. Wang,H.Y. Gao,C.Y. Wang,Z.L. Zhou,X.Y. Cai,X.X. Wang,Z.S. Zhang,K.B. Wang. 2015

[9]Heterologous Expression Of The Cotton N.bs-Lrr Gene Gbana1 Enhances V erticillium Wilt Resistance In Arabidopsis. Li, NY, Zhou, L, Zhang, DD, Klosterman, SJ, Li, TG, Gui, YJ, Kong, ZQ, Ma, XF, Short, DPG, Zhang, WQ, Li, JJ, Subbarao, KV, Chen, JY, Dai, XF. 2018

[10]Co-expression of multi-gene in cotton promotes the aggregation of multi-resistance and yield traits. Peng Wang,Hang Zhao,Xingxing Liu,Guilin Li,Xiaoshuang Zhang,Xueyan Zhang,Yadi Xing. 2023

[11]Uridine Kinase-like Protein (GhUKL4) Positively Regulates Resistance to Verticillium Wilt in Cotton. Baimei Cheng,Yanmeng Sun,Xiaohui Sang,Jianhua Lu,Pei Zhao,Wei Chen,Yunlei Zhao,Hongmei Wang. 2025

[12]A putative maize zinc-finger protein gene, ZmAN13, participates in abiotic stress response. Xuan, Ning,Jin, Ying,Zhang, Hongwei,Wang, Guoying,Xie, Yuanhong,Liu, Yunjun,Wang, Guoying.

[13]Genetic diversity analysis of abiotic stress response gene TaSnRK2.7-A in common wheat. Zhang, Hongying,Mao, Xinguo,Zhang, Jianan,Chang, Xiaoping,Jing, Ruilian,Zhang, Hongying,Wang, Chengshe.

[14]Overexpression of a Wheat CCaMK Gene Reduces ABA Sensitivity of Arabidopsis thaliana During Seed Germination and Seedling Growth. Yang, Cui,Kang, Zhensheng,Yang, Cui,Kang, Zhensheng,Yang, Cui,Li, Aili,Zhang, Zenglin,Zhu, Yuanfang,Tan, Xiaomei,Guo, Hanzi,Zhang, Xueyong,Mao, Long,Zhao, Yongliang,Geng, Shuaifeng.

[15]Genome-wide identification and analysis of abiotic stress responsiveness of the mitogen-activated protein kinase gene family in Medicago sativa L.. Hao Liu,Xianyang Li,Fei He,Mingna Li,Yunfei Zi,Ruicai Long,Guoqing Zhao,Lihua Zhu,Ling Hong,Shiqing Wang,Junmei Kang,Qingchuan Yang,Lin Chen. 2024

[16]Molecular Characteristics and Expression Patterns of Carotenoid Cleavage Oxygenase Family Genes in Rice (Oryza sativa L.). Hanjing Dai,Hao Ai,Yingrun Wang,Jia Shi,Lantian Ren,Jieqin Li,Yulu Tao,Zhaoshi Xu,Jiacheng Zheng. 2024

[17]Functional Identification Reveals That TaTGA16-2D Promotes Drought and Heat Tolerance. Jingna Ru,Jiamin Hao,Xiaoqian Ji,Bingqing Hao,Jiale Yang,Hongtao Wang,Baoquan Quan,Pengyan Guo,Jiping Zhao,Chao Wang,Huawei Shi,Zhaoshi Xu. 2025

[18]Comprehensive profiling of Bcl-2-associated athanogene (BAG) genes and their genetic potential role under cold stress in Cotton. Aamir Ali Abro,Cong Sun,Mubashir Abbas,Qiankun Liu,Zheng Jie,Yanchao Xu,Yuqing Hou,Zhongli Zhou,Rashid Iqbal,Fang Liu,Xiaoyan Cai. 2025

[19]Genome-wide identification, expression analysis, and response to abiotic stress of the phosphate transporter gene family in cucumber (Cucumis sativus L.). Kunhao Xie,Mintao Sun,Xiaoqin Wang,Xiaoya Zhou,Qinghua Di,Yang Li,Guoxiu Wu,Yansu Li,Chaoxing He,Shengli Li. 2025

[20]Genome-wide identification and analysis of the abiotic stress responsiveness of the heat shock protein 90 gene family in Medicago sativa L. Liu, Hao,Zhang, Yuqi,Li, Xianyang,Zhao, Li,Ma, Xinyue,He, Fei,Li, Mingna,Wang, Xue,Long, Ruicai,Kang, Junmei,Yang, Qingchuan,Chen, Lin. 2025

作者其他论文 更多>>