文献类型： 外文期刊
作者： Yang, Guixiang;Yan, Zixiang;Wu, Xiaoqing;Zhang, Meng;Xu, Chunlong;Shi, Linyu;Yang, Hui;Fang, Kailun
作者机构：
期刊名称： GENE THERAPY
ISSN： 0969-7128
年卷期： 2023 年
页码：
收录情况： SCIE（2023版）
摘要： The conversion of non-neuronal cells to neurons is a promising potential strategy for the treatment of neurodegenerative diseases. Recent studies have reported that shRNA-, CasRx-, or ASO-mediated Ptbp1 suppression could reprogram resident astrocytes to neurons. However, some groups have disputed the interpretation of the data underlying the reported neuron conversion events. These controversies surrounding neuron conversion may be due to differences in the astrocyte fate-mapping systems. Here, we suppressed Ptbp1 using Cas13X and labelled astrocytes with an HA tag fused to Cas13X (Cas13X-NLS-HA). We found no astrocyte-to-neuron conversion in the mouse striatum via the HA-tagged labelling system compared with the GFAP-driven tdTomato labelling system (AAV-GFAP::tdTomato-WPRE) used in previous studies. Our findings indicate that Cas13X-mediated Ptbp1 knockdown failed to induce neuron conversion in vivo.
分类号：
- 相关文献
作者其他论文 更多>>
-
Long-term evidence that controlled-release urea enhances yield and soil fertility while mitigating environmental impacts in double-season rice
作者：Fu, Yifan;Yang, Wei;Zhang, Meng;Wu, Xiangning;Yang, Guodong;Xiang, Hongshun;Du, Chanchan;Zhu, Jinjuan;Yang, Chen;Wan, Yunfan;Peng, Shaobing;Li, Yu'e;Wang, Bin;Yuan, Shen
关键词：Double-season rice;Long-term experiment;Resin-coated controlled-release urea;Soil fertility;Yield
-
DerivaPredict: A User-Friendly Tool for Predicting and Evaluating Active Derivatives of Natural Products
作者：Song, Yu;Zhang, Meng;Chang, Sihao;Chu, Ganghui;Ji, Hongchao
关键词：（1-1-2）natural product derivatives;in silico molecular design;software engineering
-
Study of regulated deficit irrigation regime based on individual fruit weight and quality response to water deficit duration: A case study in tomato
作者：Zhang, Xianbo;Yang, Hui;Du, Taisheng
关键词：Saline soil;Regulated deficit irrigation;Tomato fruit quality;Water productivity
-
Advancing novel veterinary vaccines: From comprehensive antigen and adjuvant design to preparation process optimization
作者：Zhang, Meng;Wang, Chunxin;Pan, Junqian;Cui, Haixin;Zhao, Xiang
关键词：veterinary vaccines;; preparation process;; antigen design;; adjuvant
-
Characterization and feature selection of volatile metabolites in Yangxian pigmented rice varieties through GC-MS and machine learning algorithms
作者：Cheng, Kaiqi;Dong, Ruonan;Pan, Fei;Su, Wen;Xi, Lingjie;Zhang, Meng;Geng, Jingzhang;Gao, Ruichang;Jin, Wengang;Abd El-Aty, A. M.
关键词：pigmented rice;metabolites;multivariate statistics;machine learning;volatiles
-
The chimeric gene orf610a reduces cotton pollen fertility by impairing the assembly of ATP synthase
作者：Han, Yang;Ge, Lishuang;Feng, Juanjuan;Zhang, Meng;Zhang, Hexuan;Shi, Lin;Wu, Di;Zhang, Xuexian;Guo, Liping;Qi, Tingxiang;Tang, Huini;Qiao, Xiuqin;Xing, Chaozhu;Wu, Jianyong
关键词：cotton;CMS-D2;RNA-seq;orf610a;ATP synthase;ROS
-
Graph-sequence enhanced transformer for template-free prediction of natural product biosynthesis
作者：Cong, Shan;Zhang, Meng;Song, Yu;Chang, Sihao;Tian, Jing;Zeng, Hongji;Ji, Hongchao
关键词：（0-1-3）