数字农科院2.0

Recombinant pseudorabies virus expressing the consensus VP2 protein of porcine parvovirus 1 (PPV1) protects pigs against pseudorabies virus and PPV1

文献类型： 外文期刊

作者： Tian, Xiaoxiao;Wang, Haojie;Song, Hao;Wei, Ziyi;Zhu, Xulong;Liu, Guoqing;Sun, Mingxia;Huang, Xinyi;Chen, Meng;Tang, Yandong;Wang, Haiwei;Yang, Yongbo;An, Tongqing

作者机构：

关键词： PRV variant;PPV1;consensus VP2;genetic stability;chimeric viral vector vaccine

期刊名称： VETERINARY RESEARCH

ISSN： 0928-4249

年卷期： 2025 年 56 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Pseudorabies virus (PRV) and porcine parvovirus type 1 (PPV1) are major pathogens that cause reproductive disorders in sows, and mixed infections are frequently detected on pig farms. In this study, a recombinant PRV (rPRV-VP2) based on a PRV variant (HLJ8) was constructed to express the PPV1 VP2 protein. To ensure safety, four virulence genes (TK, gE, gI, and UL39) of PRV HLJ8 were deleted, followed by further evaluation of safety and immunogenicity in piglets. The PPV1 VP2 expression cassette was inserted into the gE/gI deletion site, enabling successful expression of the VP2 protein, which exhibited hemagglutination activity with a titre of 1:64. Animal experiments demonstrated that rPRV-VP2 elicited robust antibody responses against both PRV and PPV1, providing complete protection in both mice and piglets. Compared with commercial PRV and PPV1 vaccines, rPRV-VP2 conferred comparable or superior protection, resulting in not only no clinical symptoms but also significantly reduced viral loads in tissues. The deletion of the gE gene can distinguish wild-type virus infection from vaccine immunity in clinical applications. Overall, rPRV-VP2 offers a safe and effective strategy for dual protection against PRV and PPV1, reducing vaccination costs in pig production.

分类号：

  • 相关文献

[1]Effects of Different Conservation Methods on the Genetic Stability of Potato Germplasm. Bai, J. M.,Chen, X. L.,Lu, X. X.,Xin, X.,Zhang, Z. E.,Zhang, J. M.,Bai, J. M.,Sui, Q. J.,Liu, X. C.,Sun, B. Sh..

[2]Identification of a highly successful cryopreservation method (droplet-vitrification) for petunia. Zhang, Jin-Mei,Chen, Xiao-Ling,Huang, Bin,Zhou, Yuan-Chang,Zhang, Xiao-Ning,Volk, Gayle M..

[3]Selection of cold tolerant somaclonal variant from Citrus sinensis cv. Jincheng and genetic stability evaluation of its cold tolerance. Lin, DB,Yan, QS,Shen, DX. 1999

[4]Mapping of quantitative trait loci for kernel row number in maize across seven environments. Lu, Ming,Xie, Chuan-Xiao,Li, Xin-Hai,Hao, Zhuan-Fang,Li, Ming-Shun,Weng, Jian-Feng,Zhang, De-Gui,Bai, Li,Zhang, Shi-Huang,Lu, Ming.

[5]The rPRRSV-E2 strain exhibited a low level of potential risk for virulence reversion. Yifeng Jiang,Fei Gao,Liwei Li,Yanjun Zhou,Wu Tong,Lingxue Yu,Yujiao Zhang,Kuan Zhao,Haojie Zhu,Changlong Liu,Guoxin Li,Guangzhi Tong. 2023

[6]Engineering Zymomonas mobilis for improving genetic transformation and stability of multi-gene biosynthetic pathways. Yuhuan Huang,Xiaojie Wang,Mao Chen,Yanran Wu,Tatsaporn Todhanakasem,Nan Peng,Mingxiong He,Bo Wu. 2025

[7]Multi-trait improvement of sorghum: co-expression of bar, mcry1Ab, and mvip3A enhances herbicide tolerance and pest resistance. 刘璇,,杨江涛,,崔宇欣,,张晓春,,王旭静,,王志兴. 2025

作者其他论文 更多>>