数字农科院2.0

A plant viral effector disrupts ALD1-OSB1 immunity module to suppress chloroplast defenses

文献类型： 外文期刊

作者： Pan, Zuxian;Wang, Yaqin;Li, Fangfang;Mei, Yuzhen;Zhou, Xueping

作者机构：

关键词： ALD1;beta C1;betasatellite;chloroplast immunity;geminivirus;pipecolic acid

期刊名称： JOURNAL OF INTEGRATIVE PLANT BIOLOGY

ISSN： 1672-9072

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版）

摘要： Chloroplasts are central to plant immunity, with the chloroplast-localized protein AGD2-LIKE DEFENSE RESPONSE PROTEIN 1 (ALD1) playing a critical role in producing pipecolic acid (Pip), a key immune signal. However, the regulation of ALD1 and how pathogens evade ALD1-mediated defenses remain poorly understood. Using the geminivirus tomato yellow leaf curl China virus and its associated betasatellite (TYLCCNV/TYLCCNB) as a model, we uncovered a defense mechanism involving organellar single-stranded DNA-binding protein 1 (OSB1), which stabilizes ALD1 and promotes Pip biosynthesis to strengthen immunity. Crucially, the viral beta C1 effector encoded by TYLCCNB disrupts this pathway by binding OSB1 and sequestering it away from chloroplasts, thereby blocking OSB1-ALD1 interaction, destabilizing ALD1, and suppressing Pip-dependent defenses. Strikingly, beta C1 mutants defective in OSB1 binding fail to interfere with the OSB1-ALD1 stability, and TYLCCNV infections carrying these mutants induce attenuated symptoms in Nicotiana benthamiana. Our study not only reveals how ALD1-OSB1 cooperates in chloroplast immunity but also demonstrates how geminiviruses, as a tractable model, can dissect pathogen counter-defense strategies.

分类号：

  • 相关文献

[1]Tobacco RING E3 Ligase NtRFP1 Mediates Ubiquitination and Proteasomal Degradation of a Geminivirus-Encoded beta C1. Hu, Tao,Bao, Min,Cao, Linge,Song, Fengmin,Zhou, Xueping,Bao, Min,Zhou, Xueping,Zhang, Huawei,Xie, Qi,Shen, Qingtang. 2016

[2]Geminivirus satellite-encoded beta C1 activates UPR, induces bZIP60 nuclear export, and manipulates the expression of bZIP60 downstream genes to benefit virus infection. Zhang, Mingzhen,Cao, Buwei,Zhang, Hui,Fan, Zaifeng,Zhou, Xueping,Li, Fangfang. 2022

[3]Effect of Altitude on the Release of Metabolites in the Tobacco Rhizosphere Soil. Gao, L.,Gao, J. M.,Wang, R.,Ren, X. H.,Fan, J.,Shen, G. M.. 2022

[4]Tomato Yellow Leaf Curl China V.irus Impairs Photosynthesis In T he Infected Nicotiana Benthamiana With Beta C1 As An Aggravating Factor. Yang, Qiuying,Zhou, Xueping,Zhou, Xueping,Yang, Qiuying,Farooq, Tahir,Liu, Dandan. 2019

[5]Genomic Analysis of the Natural Population of Wheat dwarf virus in Wheat from China and Hungary. Liu Yan,Wang Biao,Wu Bei-lei,Wang Xi-feng,Liu Yan,Wu Yuan-hua,Vida, Gyula,Cseplo-Karolyi, Monika. 2012

[6]Mimic Phosphorylation of a beta C1 Protein Encoded by TYLCCNB Impairs Its Functions as a Viral Suppressor of RNA Silencing and a Symptom Determinant. Zhong, Xueting,Wang, Zhan Qi,Xiao, Ruyuan,Cao, Linge,Wang, Yaqin,Xie, Yan,Zhou, Xueping,Zhou, Xueping.

[7]The AC5 protein encoded by Mungbean yellow mosaic India virus is a pathogenicity determinant that suppresses RNA silencing-based antiviral defenses. Xu, Xiongbiao,Huang, Changjun,Gu, Zhouhang,Cao, Linge,Hu, Tao,Li, Zhenghe,Zhou, Xueping,Li, Fangfang,Zhou, Xueping,Ding, Ming.

[8]Geminiviruses And Their Application In B.iotechnology. Zhou Xue-ping,Ding Bo,Yang Qiu-ying. 2017

[9]F-actin dynamics in midgut cells enables virus persistence in vector insects. Wang, Hui,Liu, Yan,Liu, Wenwen,Wu, Kongming,Wang, Xifeng. 2022

[10]The C4 protein encoded by Tomato leaf curl Yunnan virus interferes with mitogen-activated protein kinase cascade-related defense responses through inhibiting the dissociation of the ERECTA/BKI1 complex. Yuzhen Mei,Yaqin Wang,Tao Hu,Zifu He,Xueping Zhou. 2021

[11]Iterons Homologous To Helper Geminiviruses A.re Essential For Efficient R eplication Of Betasatellites. Xu, XB, Qian, YJ, Wang, YG, Li, ZH, Zhou, XP. 2019

[12]Nucleocytoplasmic Shuttling Of Geminivirus C4 P.rotein Mediated By Phosphorylation A nd Myristoylation Is Critical For Viral Pathogenicity. Hu, Tao,Zhou, Xueping,Zhou, Xueping,Lozano-Duran, Rosa,Wang, Yaqin,Mei, Yuzhen,Sunter, Garry,Yang, Xiuling. 2018

[13]RepA promotes the nucleolar exclusion of the V2 protein of mulberry mosaic dwarf-associated virus. Zhou, Xueping,Ren, Yanxiang,Ren, Yanxiang,Yang, Xiuling,Zhou, Xueping,Li, Shifang,Wang, Dongxue,Sun, Shaoshuang,Ren, Yanxiang. 2020

[14]Molecular Characterization and Pathogenicity of a Novel Soybean-Infecting Monopartite Geminivirus in China. Min Du,Yongzhi Wang,Cheng Chen,Xiaoyu Li,Runzi Feng,Xueping Zhou,Xiuling Yang. 2022

[15]ty-5 Confers Broad-Spectrum Resistance to Geminiviruses. Yanxiang Ren,Xiaorong Tao,Dawei Li,Xiuling Yang,Xueping Zhou. 2022

[16]Geminiviral C2 proteins inhibit active autophagy to facilitate virus infection by impairing the interaction of ATG7 and ATG8. Cao, Buwei,Ge, Linhao,Zhang, Mingzhen,Li, Fangfang,Zhou, Xueping. 2023

[17]Molecular characterization and pathogenicity of a novel monopartite geminivirus infecting tobacco in China. Yuan Chen,Shiping Guo,Lianqiang Jiang,Fangfang Yan,Kaiqiang Hao,Zhiping Wang,Mengnan An,Zihao Xia,Fangfang Li,Xueping Zhou,Yuanhua Wu. 2024

[18]Two viral proteins translated from one open reading frame target different layers of plant defense. Mei, Yuzhen,Hu, Tao,Wang, Yaqin,Lozano-Duran, Rosa,Yang, Xiuling,Zhou, Xueping. 2024

[19]Identification of Interaction Between Geminivirus and Plant Proteins by Bimolecular Fluorescence Complementation (BiFC). Min Du,Xueping Zhou,Xiuling Yang. 2025

[20]Geminivirus βV1- protein activates bZIP17/28mediated UPR signaling to facilitate viral pathogenicity but its activity is attenuated by autophagic degradation in plants. Hu, Tao,Li, Chenyang,Liu, Hui,Su, Chenlu,Wang, Yaqin,Li, Fangfang,Zhou, Xueping. 2025

作者其他论文 更多>>