数字农科院2.0

Immunogenicity and Protective Capacity of Sugar ABC Transporter Substrate-Binding Protein against Streptococcus suis Serotype 2, 7 and 9 Infection in Mice

文献类型： 外文期刊

作者： Zujie Yan;Ruyi Pan;Junjie Zhang;Jianhe Sun;Xiaochun Ma;Nihua Dong;Xiaohui Yao;Jianchao Wei;Ke Liu;Yafeng Qiu;Katie Sealey;Hester Nichols;Michael A. Jarvis;Mathew Upton;Xiangdong Li;Zhiyong Ma;Juxiang Liu;Beibei Li

作者机构：

关键词： serotypes;Streptococcus suis;subunit vaccine;sugar ABC transporter substrate-binding protein;universal vaccine

期刊名称： Vaccines

ISSN： 2076-393X

年卷期： 2024 年 12 卷 5 期

页码：

收录情况： SCIE（2024版）

摘要： Background: Streptococcus suis (S. suis) is a Gram-positive bacterium that causes substantial disease in pigs. S. suis is also an emerging zoonoses in humans, primarily in Asia, through the consumption of undercooked pork and the handling of infected pig meat as well as carcasses. The complexity of S. suis epidemiology, characterized by the presence of multiple bacterial serotypes and strains with diverse sequence types, identifies a critical need for a universal vaccine with the ability to confer cross-protective immunity. Highly conserved immunogenic proteins are generally considered good candidate antigens for subunit universal vaccines. Methods: In this study, the cross-protection of the sugar ABC transporter substrate-binding protein (S-ABC), a surface-associated immunogenic protein of S. suis, was examined in mice for evaluation as a universal vaccine candidate. Results: S-ABC was shown to be highly conserved, with 97% amino acid sequence identity across 31 S. suis strains deposited in GenBank. Recombinantly expressed S-ABC (rS-ABC) was recognized via rabbit sera specific to S. suis serotype 2. The immunization of mice with rS-ABC induced antigen-specific antibody responses, as well as IFN-γ and IL-4, in multiple organs, including the lungs. rS-ABC immunization conferred high (87.5% and 100%) protection against challenges with S. suis serotypes 2 and 9, demonstrating high cross-protection against these serotypes. Protection, albeit lower (50%), was also observed in mice challenged with S. suis serotype 7. Conclusions: These data identify S-ABC as a promising antigenic target within a universal subunit vaccine against S. suis.

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