数字农科院2.0

In-house Developed ELISA Indicates High Prevalence of anti-Echinococcus granulosus IgG in Sheep Population – An Update from Pakistan

文献类型： 外文期刊

作者： Alvi MA; Ohiolei JA; Li L; Saqib M; Hussain MH; Tayyab MH; Ghafoor M; Qamar W; Faridi NY; Alvi AA; Fu BQ; Yan HB; Jia WZ

关键词： Echinococcus granulosus; ELISA; epidemiology; seroprevalence; sheep; Pakistan; risk factor

期刊名称： Pathogens

ISSN： 2076-0817

年卷期： 2020 年

页码：

收录情况： JCR（2021版）

摘要： Cystic echinococcosis (CE) is a World Health Organization (WHO)-listed neglected tropical farm economy jeopardizing and public health concern disease. This study was aimed at furnishing sero-epidemiological baseline data of CE in sheep in Pakistan, where data are non-existent. For this purpose, two sheep-rich provinces of Pakistan were selected, and 728 sheep sera were collected using probability proportional to size (PPS) statistical technique. Epidemiological information was recorded on a questionnaire for the estimation of potential risk factors. The serum samples were analyzed for IgG antibodies against Echinococcus granulosus using an in-house-developed EgAgB-based ELISA kit. The overall seroprevalence recorded was 21.98% (160/728) in the tested sheep, suggesting higher seropositivity in sheep from Punjab (23.73%) as compared to Khyber Pakhtunkhwa (KPK) (19.04%). The overall apparent prevalence observed by this ELISA method was almost similar to the calculated true prevalence (21.77%). Prevalence was significantly different (p < 0.05) among sheep from different districts. Higher prevalence was found in females (22.54%, OR 1.41), age group > 5 years (29.66%, OR 1.64), crossbreeds (42.85%, OR 2.70), and sheep with pasture access (25.96%, OR 3.06). Being in age group > 5 years and having pasture access were the factors significantly associated with seropositivity (p < 0.05). This study provides serological evidence of E. granulosus infection in sheep and can be used as a model for ante-mortem screening of the sheep globally.

分类号：

  • 相关文献

[1]First Report Of Bovine Viral D.iarrhea Virus And Mycobacterium A vium Subspecies Paratuberculosis Infection In Tibetan Sheep (Ovis Aries) In Tibetan Plateau, China. Ma, JG, Tian, AL, Zheng, WB, Zou, Y, Zhang, YT, Yang, ZQ. 2019

[2]Echinococcus granulosus (sensu stricto) (G1, G3) and E. ortleppi (G5) in Pakistan: phylogeny, genetic diversity and population structural analysis based on mitochondrial DNA. 贾万忠, 闫鸿斌,,李立,,付宝权. 2020

[3]Dengue Fever Is An Emerging P.ublic Health Concern In T he City Of Multan, Pakistan: Its Seroprevalence And Associated Risk Factors. Mukhtar, MU, Mukhtar, M, Iqbal, N. 2018

[4]Seroprevalence Of Toxoplasma Gondii In S.outh Asian Countries. Khan, MU, Rashid, I, Akbar, H, Islam, S, Riaz, F, Nabi, H, Ashraf, K, Singla, LD. 2017

[5]Seroprevalence And Risk Factors Of F.ascioliasis In Yaks, Bos G runniens, From Three Counties Of Gansu Province, China. Zhang, XX, Feng, SY, Ma, JG, Zheng, WB, Yin, MY, Qin, SY, Zhou, DH, Zhao, Q, Zhu, XQ. 2017

[6]Identification Of Anaplasma Ovis Appendage-Associated P.rotein (Aaap) For Development O f An Indirect Elisa And Its Application. Wang, ZG, Yang, JF, Niu, QL, Brayton, KA, Luo, JX, Liu, GY, Yin, H, Liu, ZJ. 2017

[7]Epidemiological Investigation And Phylogenetic Analysis O.f Caprine Parainfluenza Virus T ype 3 In Sheep Of China. Mao, L, Yang, LL, Li, WL, Liang, PH, Zhang, SH, Li, JZ, Sun, M, Zhang, WW, Wang, LQ, Zhong, CY, Liu, MJ, Jiang, JY, Cai, XP, Luo, XN. 2019

[8]产气荚膜梭菌β1毒素蛋白的原核表达及其间接ELISA方法的建立. 张广林,徐龙,高云艳,李玲霞,刘永生,尚佑军,曹小安. 2021

[9]中国西部和北部边境4省区牛支原体血清流行病学调查及分析. 李媛,闫磊,刘桐,辛九庆. 2021

[10]非洲猪瘟病毒p72蛋白间接ELISA抗体检测方法的建立. 庚辛,孙明霞,王淑杰,汤艳东,高金源,蔡雪辉. 2021

[11]Selenium distribution, translocation and speciation in wheat (Triticum aestivum L.) after foliar spraying selenite and selenate. Di X., Qin X., Zhao L., Liang X., Xu Y., Sun Y., Huang Q.. 2023

[12]Speed of Movement, Fatness, and the Change in Cardiometabolic Risk Factors in Children. Shang X., Li Y., Xu H., Zhang Q., Liu A., Ma G.. 2022

[13]Spatial distribution of bacterial resistance towards antibiotics of rural sanitation system in China and its potential link with diseases incidence. Li H., Tan L., Zhang C., Wei X., Wang Q., Li Q., Zheng X., Xu Y.. 2023

[14]Effects of chilling rate on progression of rigor mortis in postmortem lamb meat. Yan T., Hou C., Wang Z., Li X., Chen L., Liang C., Xu Y., Zhang D.. 2022

[15]A naphthimide fluorescent probe for the detection of selenols in selenium-enriched Tan sheep. Liu Y., Feng X., Meng Q., Zhu J., Jia X., Zhao Q., Tang C., Yu Y., Zhang J.. 2022

[16]Characterization of Shiga toxin-producing Escherichia coli isolated from Cattle and Sheep in Xinjiang province, China, using whole-genome sequencing. Liu Y., Li H., Chen X., Tong P., Zhang Y., Zhu M., Su Z., Yao G., Li G., Cai W.. 2022

[17]Genetic variation of the 8-kDa glycoprotein family from Echinococcus granulosus, Taenia multiceps and Taenia hydatigena. Jia Wan-zhong,Yan Hong-bin,Lou Zhong-zi,Ni Xing-wei,Liu Hong-xia,Li Hong-min,Guo Ai-jiang,Fu Bao-quan. 2011

[18]Proteomic Analysis Of Taenia Hydatigena C.yst Fluid Reveals Unique I nternal Microenvironment. Zheng, YD. 2017

[19]Herbal Medicines against Hydatid Disease: A Systematic Review (2000–2021). Mughees Aizaz Alvi,Sadiq Khan,Rana Muhammad Athar Ali,Warda Qamar,Muhammad Saqib,Noman Yousaf Faridi,Li Li,Bao Quan Fu,Hong Bin Yan,Wan Zhong Jia. 2022

[20]Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences. Shumuye, Nigus Abebe,Li, Li,Ohiolei, John Asekhaen,Qurishi, Sayed Ajmal,Li, Wen-Hui,Zhang, Nian-Zhang,Wu, Yan-Tao,Wu, Yao-Dong,Gao, Sheng-Zhi,Zhang, Fu-Heng,Tian, Xue-Qi,Tian, Wen-Jun,Fu, Yong,Wang, Xie-Zhong,Pan, Yong-Hong,Zhan, Fang,Zhang, Lin-Sheng,Guo, Ming-Kuan,Li, Wen-Dong,Fu, Bao-Quan,Yan, Hong-Bin,Jia, Wan-Zhong. 2023

作者其他论文 更多>>