数字农科院2.0

Lactiplantibacillus plantarum FRT4 protects against fatty liver hemorrhage syndrome: regulating gut microbiota and FoxO/TLR-4/NF-κB signaling pathway in laying hens

文献类型： 外文期刊

作者： Daojie Li;Kun Meng;Guohua Liu;Zhiguo Wen;Yunsheng Han;Weiwei Liu;Xin Xu;Liye Song;Hongying Cai;Peilong Yang

作者机构：

关键词： Fatty liver hemorrhage syndrome;FoxO/TLR-4/NF-κB signaling pathway;Gut microbiota;Lactiplantibacillus plantarum FRT4;Oxidative stress

期刊名称： Microbiome

ISSN： 2049-2618

年卷期： 2025 年 13 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Background: Fatty liver hemorrhage syndrome (FLHS) has become one of the major factors leading to the death of laying hen in caged egg production. FLHS is commonly associated with lipid peroxidation, hepatocyte injury, decreased antioxidant capacity, and inflammation. However, there are limited evidences regarding the preventive effect of Lactiplantibacillus plantarum on FLHS in laying hens and its mechanisms. Our previous results showed that Lp. plantarum FRT4 alleviated FLHS by regulating lipid metabolism, but did not focus on its antioxidant and anti-inflammatory functions and mechanisms. Therefore, this study aimed to investigate the preventive mechanisms of Lp. plantarum FRT4 in alleviating FLHS, with a focus on its role in antioxidant activity and inflammation regulation. Results: Supplementation with Lp. plantarum FRT4 enhanced the levels of T-AOC, T-SOD, and GSH-Px, while reducing the levels of TNF-α, IL-1β, IL-8, and NLRP3 in the liver and ovary of laying hens. Additionally, Lp. plantarum FRT4 upregulated the mRNA expressions of SOD1, SOD2, CAT, and GPX1, downregulated the mRNA expressions of pro-inflammatory factors IL-1β, IL-6, and NLRP3, and upregulated the mRNA expressions of anti-inflammatory factors IL-4 and IL-10. Lp. plantarum FRT4 improved the structure and metabolic functions of gut microbiota, and regulated the relative abundances of dominant phyla (Bacteroidetes, Firmicute, and Proteobacteria) and genera (Prevotella and Alistipes). Additionally, it influenced key KEGG pathways, including tryptophan metabolism, amino sugar and nucleotide sugar metabolism, insulin signaling pathway, FoxO signaling pathway. Spearman analysis revealed that the abundance of microbiota at different taxonomic levels was closely related to antioxidant enzymes and inflammatory factors. Furthermore, Lp. plantarum FRT4 modulated the mRNA expressions of related factors in the FoxO/TLR-4/NF-κB signaling pathway by regulating gut microbiota. Moreover, the levels of E2, FSH, and VTG were significantly increased in the ovary after Lp. plantarum FRT4 intervention. Conclusions: Lp. plantarum FRT4 effectively ameliorates FLHS in laying hens. This efficacy is attributed to its antioxidant and anti-inflammatory properties, which are mediated by modulating the structure and function of gut microbiota, and further intervening in the FoxO/TLR-4/NF-κB signaling pathway. These actions enhance hepatic and ovarian function and increase estrogen levels. 7zWWtwaUetzY3M_g_ZYGWGVideo Abstract

分类号：

  • 相关文献

[1]Lactiplantibacillus plantarum FRT4 attenuates high-energy low-protein diet-induced fatty liver hemorrhage syndrome in laying hens through regulating gut-liver axis. Li D.,Cai H.,Liu G.,Han Y.,Qiu K.,Liu W.,Meng K.,Yang P.. 2024

[2]Hydroxytyrosol attenuates diquat-induced oxidative stress by activating Nrf2 pathway and modulating colonic microbiota in mice. Hui Han,Ruqing Zhong,Shunfen Zhang,Mengyu Wang,Xiaobin Wen,Bao Yi,Yong Zhao,Liang Chen,Hongfu Zhang. 2023

[3]Supplementation With Chinese Medicinal Plant Extracts From Lonicera hypoglauca and Scutellaria baicalensis Mitigates Colonic Inflammation by Regulating Oxidative Stress and Gut Microbiota in a Colitis Mouse Model. Fan Wan,Mengyu Wang,Ruqing Zhong,Liang Chen,Hui Han,Lei Liu,Yong Zhao,Huiyuan Lv,Fujiang Hou,Bao Yi,Hongfu Zhang. 2022

[4]Selenomethionine Ameliorates Cognitive Impairment, Decreases Hippocampal Oxidative Stress and Attenuates Dysbiosis in D-Galactose-Treated Mice. Ying Gao,Yongquan Xu,Junfeng Yin. 2022

[5]The Interaction between Oxidative Stress Biomarkers and Gut Microbiota in the Antioxidant Effects of Extracts from Sonchus brachyotus DC. in Oxazolone-Induced Intestinal Oxidative Stress in Adult Zebrafish. Yang J.,Zhou W.-W.,Shi D.-D.,Pan F.-F.,Sun W.-W.,Yang P.-L.,Li X.-M.. 2023

[6]Cereal polyphenols inhibition mechanisms on advanced glycation end products and regulation on type 2 diabetes. Dong, Lezhen,Li, Ying,Chen, Qin,Liu, Yahui,Wu, Zufang,Pan, Daodong,Yan, Ning,Liu, Lianliang. 2023

[7]Effects of tea against alcoholic fatty liver disease by modulating gut microbiota in chronic alcohol-exposed mice. Bangyan Li,Qianqian Mao,Dandan Zhou,Min Luo,Renyou Gan,Hangyu Li,Siyu Huang,Adila Saimaiti,Ao Shang,Huabin Li. 2021

[8]Hydroxytyrosol Alleviates Intestinal Oxidative Stress by Regulating Bile Acid Metabolism in a Piglet Model. Xiaobin Wen,Fan Wan,Ruqing Zhong,Liang Chen,Hongfu Zhang. 2024

[9]Sika Deer Velvet Antler Peptide Exerts Neuroprotective Effect in a Parkinson’s Disease Model via Regulating Oxidative Damage and Gut Microbiota. Ying Liu,Hongyuan Li,Min Yang,Jia Guo,Zepeng Sun,Shuyue Wang,Ru Li,Xin Pang,Yumi Kim,Xiaohui Wang,Yinghua Peng. 2024

[10]Network Analysis of Gut Microbial Communities Reveals Key Reason for Quercetin Protects against Colitis. Yanan Lv,Jing Peng,Xiaoyu Ma,Zeyi Liang,Ghasem Hosseini Salekdeh,Qunhua Ke,Wenxiang Shen,Zuoting Yan,Hongsheng Li,Shengyi Wang,Xuezhi Ding. 2024

[11]Gut microbiota dysbiosis and oxidative damage in high-fat diet-induced impairment of spermatogenesis: Role of protocatechuic acid intervention. Hu, Ruizhi,Yang, Xizi,Wang, Long,Su, Dingding,He, Ziyu,Li, Jiaxing,Gong, Jiatai,Zhang, Wentao,Ma, Siqi,Shi, Mingkun,Lv, Jing,Zhang, Qianjin,Hou, De-Xing,Zhang, Hongfu,He, Jianhua,Yin, Yulong,Wang, Jun,Wu, Shusong. 2024

[12]Mangosteen Pericarp Extract Mitigates Diquat-Induced Hepatic Oxidative Stress by NRF2/HO-1 Activation, Intestinal Barrier Integrity Restoration, and Gut Microbiota Modulation. Weichen Huang,Yujie Lv,Chenhao Zou,Chaoyue Ge,Shenao Zhan,Xinyu Shen,Lianchi Wu,Xiaoxu Wang,Hongmeng Yuan,Gang Lin,Dongyou Yu,Bing Liu. 2025

[13]Lactobacillus plantarum QL01 Alleviates D-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Ageing Mice. Li, Haichuan,Zhang, Mingqing,Wu, Diyan,Gong, Di,Huang, Jiazhang,Tang, Zhenchuang,Wang, Liang,Zhang, Ying. 2025

[14]Fufang Huangqi Jianpi Oral Liquid alleviates spleen deficiency diarrhea via modulation of the NF-κB/Nrf2 signaling pathway and apoptosis. Lina Huang,Zhengying Qiu,Ke Sun,Cong Yu,Qilin Huang,Chen Liu,Xiangyi Pan,Sifan Li,Shaoguang Ge,Xiongwei Shi,Fangdi Hu,Ruihua Xin. 2025

[15]Dietary 18β-Glycyrrhetinic Acid Supplementation Improves Intestinal Function and Gut Microbiota in D-Galactose-Challenged Weanling Pigs. Ma, Cui,Wang, Fuxi,Li, Ruitong,Huang, Kang,Zhao, Qingyu,Qin, Yuchang,Zhang, Junmin,Si, Wei. 2025

[16]Gut microbiota and bile acid profiles in purebred vs. crossbred sows: links to oxidative stress and inflammation in late gestation. Chenggang Yin,Lei Xu,Zixi Wei,Ying Zhao,Rong Bai,Ge Gao,Yuyang Fan,Yanpin Li,Wenjuan Sun,Xilong Li,Yu Pi. 2025

[17]Limosilactobacillus reuteri ZY15 Alleviates Intestinal Inflammation and Barrier Dysfunction via AKT/mTOR/HIF-1α/RORγt/IL-17 Signaling and the Gut Microbiota in ETEC K88-Challenged Mice. Xin Xu,Hongwei Zhang,Kun Meng,Hongying Cai,Weiwei Liu,Liye Song,Zihan Zhang,Qijun Zhu,Xiling Han,Yunsheng Han,Peilong Yang. 2025

[18]Sublethal aflatoxin B1 exposure triggers multidimensional damage in honeybee (Apis mellifera) midgut: Integrative evidence from histomorphology, transcriptomics, and metagenomics. Xuanpeng Liu,Jilian Li,Chuan Ma. 2026

[19]Whole Genome Sequencing and Extracellular Metabolite Profiling of Lactiplantibacillus plantarum FRT4: Insights into Probiotic Functionality. Yuyin Huang,Kun Meng,Guohua Liu,Zhimin Chen,Yunsheng Han,Peilong Yang,Rui Zhang,Hongying Cai. 2025

[20]Heat-Inactivated Lactiplantibacillus plantarum FRT4 Alleviates Diet-Induced Obesity via Gut-Liver Axis Reprogramming. Huang, Yuyin,Wang, Qingya,Han, Xiling,Meng, Kun,Liu, Guohua,Zhang, Haiou,Zhang, Rui,Cai, Hongying,Yang, Peilong. 2025

作者其他论文 更多>>