数字农科院2.0

Development of a specific anti-idiotypic nanobody for monitoring aflatoxin M1 in milk and dairy products

文献类型： 外文期刊

作者： Cai Chong;Zhang Qi;Nidiaye Seyni;Yan Honglin;Zhang Wen;Tang Xiaoqian;Li Peiwu

作者机构：

关键词： Aflatoxin M1; ELISA; Milk products; Nanobody; Phage-displayed

期刊名称： Microchemical Journal

ISSN： 0026-265X

年卷期： 2021 年 167 卷

页码：

收录情况： JCR（2021版）

摘要： Anti-idiotypic antibodies (AIds) display stable protein conformation, offering an alternative to conventional antigens in immunoassays. In this work, an anti-idiotypic variable domain of heavy-chain antibody (VHH) or nanobody named VHH C4 binding to monoclonal antibody (mAb) 2C9 against aflatoxin M1 (AFM1) was screened out from an alpaca immunized phage-displayed library. VHH C4 shows outstanding features in specificity and sensitivity, the IC50 is 0.25 ng/mL and exhibit almost no cross-reactivity with other aflatoxin analogs. As our knowledge, it is the first report for developing an anti-idiotypic nanobody for AFM1. An environment-friendly competitive ELISA was developed using VHH C4 as a coating antigen. The results revealed a linear range from 0.10 ng/mL to 0.60 ng/mL, with a limit of detection (LOD) of 0.05 ng/mL. The recovery of spiked samples (pure milk, yogurt, and milk powder) ranged from 84.1% to 119.1%. The ELISA results of AFM1 content in naturally contaminated samples were in good agreement with those determined by high performance liquid chromatography (HPLC). The results demonstrated that the nanobody VHH C4 is a promising alternative antigen in developing immunological methods for the detection of AFM1 in dairy products. © 2021

分类号：

  • 相关文献

[1]Enhancing the detection sensitivity of nanobody against aflatoxin B1 through structure-guided modification. He T., Nie Y., Yan T., Zhu J., He X., Li Y., Zhang Q., Tang X., Hu R., Yang Y., Liu M.. 2022

[2]产气荚膜梭菌β1毒素蛋白的原核表达及其间接ELISA方法的建立. 张广林,徐龙,高云艳,李玲霞,刘永生,尚佑军,曹小安. 2021

[3]中国西部和北部边境4省区牛支原体血清流行病学调查及分析. 李媛,闫磊,刘桐,辛九庆. 2021

[4]非洲猪瘟病毒p72蛋白间接ELISA抗体检测方法的建立. 庚辛,孙明霞,王淑杰,汤艳东,高金源,蔡雪辉. 2021

[5]Anti-Idiotypic Nanobody-Phage Display-Mediated Real-Time Immuno-Pcr F.or Sensitive, Simultaneous And Q uantitative Detection Of Total Aflatoxins And Zearalenone In Grains. Ren, XF, Zhang, Q, Wu, WH, Yan, TT, Tang, XQ, Zhang, W, Yu, L, Li, PW. 2019

[6]Time-Resolved Fluorescence Immunochromatography Assay (Trfica) For Aflatoxin: Aiming At Increasing Strip Method Sensitivity. Li, H, Wang, D, Tang, XQ, Zhang, W, Zhang, Q, Li, PW. 2020

[7]Development And Application Of A Novel Elisa For Detecting Antibodies Against Group I Fowl Adenoviruses. Pan, Q, Wang, J, Gao, YL, Cui, HY, Liu, CJ, Qi, XL, Zhang, YP, Wang, YQ, Li, K, Gao, L, Wang, XM. 2020

[8]Tissue Fluid Enzyme-Linked Immunosorbant Assay F.or Piglets Experimentally Infected W ith Toxoplasma Gondii And Survey On Local And Imported Pork In Korean Retail Meat Markets. Yoo, WG, Kim, SM, Won, EJ, Lee, JY, Dai, FH, Woo, HC, Nam, HW, Kim, TI, Han, JH, Kwak, D, Cho, YS, Kang, SW, Kim, TS, Zhu, XQ, Wang, CR, Youn, H, Hong, SJ. 2018

[9]No Detrimental Effect Of Bt M.aize Pollen Containing Cry1Ab/2Aj O r Cry1Ac On Adult Green Lacewings Chrysoperla Sinica Tjeder. Li Yun-he,Liu Yan-min,Peng Yu-fa,Shen Ping,Song Xin-yuan,Chen Xiu-ping. 2019

[10]Development Of An Indirect Competitive E.lisa For The Detection O f Acenaphthene And Pyrene. Qiao, B, Li, YS, Meng, XY, Sun, Y, Hu, P, Lu, SY, Ren, HL, Liu, ZS, Zhou, Y. 2017

[11]Serosurvey Of Ovine And Caprine T.heileriosis With Elisa In C hina. Li, Q, Han, Y, Liu, ZJ, Liu, JL, Liu, AH, Li, YQ, Guan, GQ, Luo, JX, Yin, H, Li, YQ. 2017

[12]Development Of An Indirect Enzyme-Linked I.mmunosorbent Assay (Elisa) To D ifferentiate Antibodies Against Wild-Type Porcine Reproductive And Respiratory Syndrome From The Vaccine Strain Tjm-F92 Based On A Recombinant Nsp2 Protein. Wang, XX, Wang, FX, Li, ZG, Wen, YJ, Wang, X, Song, N, Wu, H. 2018

[13]Development Of Luciferase-Linked Antibody Capture A.ssay Based On Luciferase I mmunoprecipitation Systems For Antibody Detection Of Porcine Reproductive And Respiratory Syndrome Virus. Li, J, Wang, G, Yang, D, Zhao, B, Zhao, YP, Liu, YG, Cai, XH, Nan, YC, Zhou, EM, Wu, CY. 2018

[14]Toxicological And Biochemical Analyses Demonstrate T.he Absence Of Lethal O r Sublethal Effects Of Cry1C- Or Cry2A-Expressing Bt Rice On The Collembolan Folsomia Candida. Li, Yunhe,Peng, Yufa,Romeis, Jorg,Romeis, Jorg,Zhang, Bing,Yang, Yan,Yang, Yan,Zhou, Xiang,Zhang, Bing. 2018

[15]Self-assembly into virus-like particles of the recombinant capsid protein of porcine circovirus type 3 and its application on antibodies detection. 王宇,王刚,段卫同,孙明霞,汪孟航,王淑杰,蔡雪辉,涂亚斌. 2020

[16]Seroprevalence And Risk Factors Of F.ascioliasis In Yaks, Bos G runniens, From Three Counties Of Gansu Province, China. Zhang, XX, Feng, SY, Ma, JG, Zheng, WB, Yin, MY, Qin, SY, Zhou, DH, Zhao, Q, Zhu, XQ. 2017

[17]Preparation Of Monoclonal Antibody And D.evelopment Of An Indirect C ompetitive Enzyme-Linked Immunosorbent Assay For Ornidazole Detection. Zhao, L, Li, JY, Li, Y, Wang, TG, Jin, XL, Wang, K, Rahman, E, Xing, Y, Ji, BP, Zhou, F. 2017

[18]Development And Application Of An I.ndirect Elisa For The D etection Of Gp45 Antibodies To Equine Infectious Anemia Virus. Du, C, Hu, Z, Hu, SD, Lin, YZ, Wang, XJ, Li, YJ. 2018

[19]A Perspective For Improving The S.ensitivity Of Detection: The A pplication Of Multi-Epitope Recombinant Antigen In Serological Analysis Of Buffalo Schistosomiasis. Lv, C, Fu, ZQ, Lu, K, Yue, RL, Wang, T, Cao, XD, Zhu, CG, Li, H, Hong, Y, Lin, JJ. 2018

[20]Does Bt Maize Expressing Cry1Ac P.rotein Have Adverse Effects O n The Parasitoid Macrocentrus Cingulum (Hymenoptera: Braconidae)?. Cai, Wan-Zhi,Bai, Shu-Xiong,Wang, Zeng-Xia,Wang, Zeng-Xia,Zhang, Tian-Tao,Li, Yun-He,He, Kang-Lai,Wang, Zhen-Ying. 2017

作者其他论文 更多>>