数字农科院2.0

Intermolecular Structure Conversion-Based G4-TDF Nanostructures Functionalized μPADs for Fluorescent Determination of Potassium Ion in Serum

文献类型： 外文期刊

作者： Mengqi Wang;Xiuli Fu;Yixuan Liu;Zhiyang Zhang;Chenyu Jiang;Dean Song

作者机构：

关键词： DNA tetrahedral nanostructure;G-quadruplex;potassium ion;structure conversion;thioflavin T

期刊名称： Biosensors

ISSN： 2079-6374

年卷期： 2025 年 15 卷 4 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Herein, we proposed a versatile G-quadruplex (G4)-tetrahedral DNA framework (G4-TDF) nanostructure functionalized origami microfluidic paper-based device (μPADs) for fluorescence detection of K+ by lighting up thioflavin T (ThT). In this work, TDF provided robust structural support for G-rich sequence in well-defined orientation and spacing to ensure high recognition efficiency, enabling sensitive fluorescence sensing on origami μPAD. After introducing ThT, the G-rich sequences extended from TDF vertices formed a parallel G4 structure, showing weak fluorescence signal output. Upon the presence of target K+, this parallel G4 structure transitioned to antiparallel G4 structure, leading to a significantly increase in fluorescence signal of ThT. Benefiting from the outstanding fluorescence enhancement characteristic of the G4 structure for ThT and excellent specificity of the G4 structure to K+ plus satisfactory recognition efficiency with the aid of TDF, this origami paper-based fluorescence sensing strategy exhibited an impressive detection limit as low as 0.2 mM with a wide range of 0.5–5.5 mM. This innovative G4-TDF fluorescence sensing was applied for the first time on μPAD, providing a simple, effective, and rapid method for K+ detection in human serum with significant potential for clinical diagnostics.

分类号：

  • 相关文献

[1]Ultrasensitive detection of lead(II) using a turn-on probe based on the use of an aptamer and a water-soluble fluorescent perylene probe. Yan, Mengmeng,Zhu, Chao,Huang, Yafei,Yan, Jiao,Chen, Ailiang,Yan, Mengmeng,Zhu, Chao,Huang, Yafei,Yan, Jiao,Chen, Ailiang,Huang, Yafei,Yan, Jiao.

[2]G-quadruplexes in genomes of viruses infecting eukaryotes or prokaryotes are under different selection pressures from hosts. Zhen Li,Sheng Hu Qian,Fan Wang,Hany I. Mohamed,Guangfu Yang,Zhen Xia Chen,Dengguo Wei. 2022

[3]In vivo dynamics and regulation of DNA G-quadruplex structures in mammals. Ze Hao Zhang,Sheng Hu Qian,Dengguo Wei,Zhen Xia Chen. 2023

[4]Plasmonic colorimetry and G-quadruplex fluorescence-based aptasensor: A dual-mode, protein-free and label-free detection for OTA. Ming Li,Youxin Qiu,Guoxing Liu,Yu Xiao,Ye Tian,Song Fang. 2024

[5]Ultrasensitive detection assay for Cronobacter sakazakii based on nucleic acid-driven aggregation-induced emission of gold nanoclusters and cascaded signal amplification. Qing Kang,Shuai qi Zhang,Tong Lin,Jin zhao Li,Chen jing Ma,Jing bo Jiao,Cheng Li,Xin jun Du,Shuo Wang. 2024

[6]Host helicase DHX36 inhibits pseudorabies virus proliferation by unwinding the G-quadruplex in the 3’UTR of IE180. Dehua Luo,Yuqing Bai,Qingling Li,Yingge Zheng,Lijun Guo,Daozhong Wang,Xi Chen,Dengguo Wei. 2025

作者其他论文 更多>>