数字农科院2.0

A new l-serine binding orphan SerBP affects indole synthesis in Pantoea ananatis

文献类型： 外文期刊

作者： Zhang, Lei;Yu, Jiajia;Zheng, Jing;Wu, Liqing;Zhou, Xinyi;Ban, Yali;Sun, Yuanhao;Zhang, Haotian;Feng, Yongjun

作者机构：

关键词： amino acid transport;indole;Pantoea ananatis YJ76;substrate-binding protein

期刊名称： JOURNAL OF BASIC MICROBIOLOGY

ISSN： 0233-111X

年卷期： 2023 年

页码：

收录情况： SCIE（2023版）

摘要： Indole is traditionally known as a metabolite of l-tryptophan and now as an important signaling molecule in bacteria, however, the understanding of its upstream synthesis regulation is very limited. Pantoea ananatis YJ76, a predominant diazotrophic endophyte isolated from rice (Oryza sativa), can produce indole to regulate various physiological and biochemical behaviors. We constructed a mutant library of YJ76 using the mTn5 transposon insertion mutation method, from which an indole-deficient mutant was screened out. Via high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR), the transposon was determined to be inserted in a gene (RefSeq: WP014605468.1) of unknown function that is highly conserved at the intraspecific level. Bioinformatics analysis implied that the protein (Protein ID: WP089517194.1) encoded by the mutant gene is most likely to be a new orphan substrate-binding protein (SBP) for amino acid ABC transporters. Amino acid supplement cultivation experiments and surface plasmon resonance revealed that the protein could bind to l-serine (K-D = 6.149 x 10(-5) M). Therefore, the SBP was named as SerBP. This is the first case that a SBP responds to l-serine ABC transports. As a precursor of indole synthesis, the transmembrane transported l-serine was directly correlated with indole signal production and the mutation of serBP gene weakened the resistance of YJ76 to antibiotics, alkali, heavy metals, and starvation. This study provided a new paradigm for exploring the upstream regulatory pathway for indole synthesis of bacteria.

分类号：

  • 相关文献

[1]Poly(A) polymerase I participates in the indole regulatory pathway of Pantoea agglomerans YS19. Li, Zihua,Jiang, Jing,Yu, Xuemei,Feng, Yongjun,Wu, Cunxiang,Shen, Delong.

[2]Indole affects the formation of multicellular aggregate structures in Pantoea agglomerans YS19. Yu, Xuemei,Jiang, Jing,Liang, Chen,Zhang, Xiao,Wang, Jieru,Feng, Yongjun,Shen, Delong.

[3]Selective Toxicity of Secondary Metabolites from the Entomopathogenic Bacterium Photorhabdus luminescens sonorensis against Selected Plant Parasitic Nematodes of the Tylenchina Suborder. Kusakabe A., 王辰., Xu Y.-M., Molnár I., Stock S.P.. 2022

[4]An Odorant Receptor from the Proboscis of the Cotton Bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) Narrowly Tuned to Indole. Guo, Mengbo,Ren, Xueting,Liu, Yang,Wang, Guirong. 2022

[5]The Effect of Lactobacillus plantarum on the Fecal Microbiota, Short Chain Fatty Acids, Odorous Substances, and Blood Biochemical Indices of Cats. Bing Han,Shukun Liang,Jintao Sun,Hui Tao,Zhenlong Wang,Baosheng Liu,Xiumin Wang,Jie Liu,Jinquan Wang. 2024

[6]Metabolic Analysis of Indole-degradated Metabolites in Two Acinetobacter spp.. T. A. Tesso,G. Liu. 2023

[7]Synthesis, Antimicrobial Activities, and Model of Action of Indolyl Derivatives Containing Amino-Guanidinium Moieties. Yu Xi Li,Xiang Geng,Qi Tao,Ruo Chen Hao,Ya Jun Yang,Xi Wang Liu,Jian Yong Li. 2025

作者其他论文 更多>>