数字农科院2.0

Simultaneous determination of protoporphyrin IX and magnesium protoporphyrin IX in Arabidopsis thaliana and Camellia sinensis using UPLC-MS/MS

文献类型： 外文期刊

作者： Zhang, Chenyu;Ma, Chunlei;Zhu, Li;Yao, Mingzhe

作者机构：

关键词： Mg-protoporphyrin IX;Protoporphyrin IX;UPLC-MS;MS;Chlorophyll biosynthesis;Camellia sinensis;Arabidopsis thaliana

期刊名称： PLANT METHODS

ISSN：

年卷期： 2023 年 19 卷 1 期

页码：

收录情况： SCIE（2023版）

摘要： BackgroundsInsertion of Mg2+ into protoporphyrin IX (PPIX) to produce magnesium-protoporphyrin IX (Mg-PPIX) was the first step toward chlorophyll biosynthesis, which not only imparts plants green pigmentation but underpins photosynthesis. Plants that blocked the conversion of PPIX to Mg-PPIX displayed yellowish or albino-lethal phenotypes. However, the lack of systematic study of the detection method and the metabolic difference between species have caused the research on chloroplast retrograde signaling controversial for a long time.ResultsAn advanced and sensitive UPLC-MS/MS strategy for determining PPIX and Mg-PPIX was established in two metabolic different plants, Arabidopsis thaliana (Columbia-0) and Camellia sinensis var. sinensis. Two metabolites could be extracted by 80% acetone (v/v) and 20% 0.1 M NH4OH (v/v) without hexane washing. Since the Mg-PPIX could be substantially de-metalized into PPIX in acidic conditions, analysis was carried out by UPLC-MS/MS with 0.1% ammonia (v/v) and 0.1% ammonium acetonitrile (v/v) as mobile phases using negative ion multiple reaction monitoring modes. Interestingly, it could be easier to monitor these two compounds in dehydrated samples rather than in fresh samples. Validation was performed in spiked samples and mean recoveries ranged from 70.5 to 916%, and the intra-day and inter-day variations were less than 7.5 and 10.9%, respectively. The limit of detection was 0.01 mg center dot kg(- 1) and the limit of quantification was 0.05 mg center dot kg(- 1). The contents of PPIX (1.67 +/- 0.12 mg center dot kg(- 1)) and Mg-PPIX (3.37 +/- 0.10 mg center dot kg(- 1)) in tea were significantly higher than in Arabidopsis (PPIX: 0.05 +/- 0.02 mg center dot kg(- 1); Mg-PPIX: 0.08 +/- 0.01 mg center dot kg(- 1)) and they were only detected in the leaf.ConclusionsOur study establishes a universal and reliable method for determining PPIX and Mg-PPIX in two plants using UPLC-MS/MS. This procedure will facilitate studying chlorophyll metabolism and natural chlorophyll production.

分类号：

  • 相关文献

[1]Synthesis of Isotopically Labeled C-13(3)-Simazine and Development of a Simultaneous UPLC-MS/MS Method for the Analysis of Simazine in Soil. Guo, Yangzhen,Zhang, Xia,Yang, Yue,Chen, Shuo,She, Dongmei,Song, Yan,Zhang, Xia,Yang, Yue,She, Gaimei.

[2]Concentration and dissipation of chlorantraniliprole and thiamethoxam residues in maize straw, maize, and soil. Zheng, Yongquan,He, Min,Jia, Hong C.,Song, Dan.

[3]Determination of beta(2)-Agonists in Porcine Urine by Molecularly Imprinted Solid Phase Extraction Followed Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Detection. Wang, Peilong,Li, Yang,Wang, Ruiguo,Zhang, Wei,Cheng, Jie,Wang, Jing,Su, Xiaoou,Ye, Zhihua,Wang, Peilong,Li, Yang,Wang, Ruiguo,Zhang, Wei,Cheng, Jie,Wang, Jing,Su, Xiaoou,Ye, Zhihua. 2013

[4]Simultaneous determination of pyrimethanil, cyprodinil, mepanipyrim and its metabolite in fresh and home-processed fruit and vegetables by a QuEChERS method coupled with UPLC-MS/MS. Liu, Xingang,Dong, Fengshou,Xu, Jun,Li, Yuanbo,Tian, Yingying,Zhang, Ying,Han, Yongtao,Zheng, Yongquan,Qin, Dongmei. 2013

[5]Analytical Strategies for LC-MS-Based Untargeted and Targeted Metabolomics Approaches Reveal the Entomological Origins of Honey. Wang, Xinran,Li, Yi,Chen, Lanzhen,Zhou, Jinhui. 2022

[6]Simultaneous Determination of 21 Sulfonamides in Poultry Eggs Using Ionic Liquid-Modified Molecularly Imprinted Polymer SPE and UPLC-MS/MS. Suo, Decheng,Zhang, Su,Song, Zhanteng,Wang, Shi,Li, Yang,Fan, Xia. 2022

[7]Transcriptome and Biochemical Analyses of a Chlorophyll-Deficient Bud Mutant of Tea Plant (Camellia sinensis). Yuanyuan Li,Chenyu Zhang,Chunlei Ma,Liang Chen,Mingzhe Yao. 2023

[8]Box-Behnken法优化人参皂苷Rg1、Re、Rb1提取工艺. 赵立春,刘继永. 2020

[9]12H-双苯并[1,3,2]-二氧磷杂八环的合成与波谱分析. 陈万义,李重九. 2003

[10]12H-双苯并[1;3;2]-二氧磷杂八环的合成与波谱分析. 陈万义,李重九. 2003

[11]他汀类药物抗寄生虫效果和用LC／MS／MS进行药代动力学的研究. 严凤,何国声,余琛,徐梅倩,黄士新. 2006

[12]河南主栽甘薯品种脱毒组培苗的生长效果综合评价. 马春业,秦素研,赵月强,孙健,孟凡奇,王清,杨森要,刘志坚,黄大华,张勇跃. 2026

[13]Mechanisms associated with the depigmentation of brown eggshells: a review. Ming Yuan Lu,Li Xu,Guang Hai Qi,Hai Jun Zhang,Kai Qiu,Jing Wang,Shu Geng Wu. 2021

[14]Analysis of egg consumption trends: Gen Z egg consumption preferences and the classification of pink-shell eggs in China. Zhou, Wenbin,Zeng, Lingsen,Chen, Ruochen,Liu, Yu,Huang, Anning,Huang, Guoxuan,Li, Junying,Xu, Guiyun,Zheng, Jiangxia. 2025

[15]Redox status regulates eggshell color by modulating protoporphyrin IX biosynthesis via the SIRT1/PGC-1α/ALAS1 axis in brown-shelled hens. Yu Fu,Mingyuan Lu,Dongkai Liu,Jianping Wang,Haijun Zhang,Guanghai Qi,Jing Wang. 2025

[16]Optimization of ultrasonic-assisted extraction of flavonoids from Lactuca indica L. cv. Mengzao and their antioxidant properties. Hao, Junfeng,Wang, Zhijun,Jia, Yushan,Sun, Lin,Fu, Zhihui,Zhao, Muqier,Li, Yuyu,Yuan, Ning,Cong, Baiming,Zhao, Lixing,Ge, Gentu. 2023

[17]Characterization and comparison of metabolites in colostrum from yaks, buffaloes, and cows based on UPLC-QTRAP-MS metabolomics. Zhang, Xueyan,Li, Changhui,Huang, Jiaxiang,Zeng, Qingkun,Li, Ling,Yang, Pan,Wang, Pengjie,Chu, Min,Luo, Jie,Zhang, Hao. 2024

[18]Heme oxygenase 1 defects lead to reduced chlorophyll in Brassica napus. Zhu, Lixia,Gao, Jie,Liu, Jie,Yi, Bin,Ma, Chaozhi,Shen, Jinxiong,Tu, Jinxing,Fu, Tingdong,Wen, Jing,Yang, Zonghui,Zeng, Xinhua.

[19]Brassinosteroid (BR) biosynthetic gene lhdd10 controls late heading and plant height in rice (Oryza sativa L.). Liu, X.,Feng, Z. M.,Zhou, C. L.,Ren, Y. K.,Mou, C. L.,Wu, T.,Yang, C. Y.,Liu, S. J.,Jiang, L.,Wan, J. M.,Wan, J. M..

[20]A pair of light signaling factors FHY3 and FAR1 regulates plant immunity by modulating chlorophyll biosynthesis. Wang, Wanqing,Tang, Weijiang,Ma, Tingting,Lin, Rongcheng,Niu, De,Jin, Jing Bo,Wang, Haiyang,Lin, Rongcheng. 2016

作者其他论文 更多>>