数字农科院2.0

Silencing microRNA172 delays fruit ripening in apple (Malus domestica) through an AP2-ERF transcriptional regulation mechanism

文献类型： 外文期刊

作者： Bin Xia;Jinquan Feng;Yarong Wang;Rongmei Wu;Hengtao Zhang;Zhe Zhou;Zhenli Yan;Andrew P. Gleave;Jia Long Yao

作者机构：

关键词： APETALA2;Ethylene;Fruit ripening;Gene silencing;Genetic plants;Malus domestica;MicroRNA172;Transcriptome analysis

期刊名称： Plant Science

ISSN： 0168-9452

年卷期： 2025 年 360 卷

页码：

收录情况： SCIE（2025版）

摘要： MicroRNA172 (miR172) regulates many processes of plant development, including flowering and fruit growth, yet its function in fruit ripening remains unclear. In this study, we demonstrate that silencing miR172 in apple (Malus domestica) delays fruit ripening, which correlates with a postponed onset of climacteric ethylene production. At harvest, miR172-silenced fruit displayed lower respiration rates than wild-type (WT) fruit, although these rates re-aligned with WT after 12 weeks of cool storage. Although at harvest miR172-silenced and WT fruit showed similar firmness and levels of soluble solid content, the miR172-silenced fruit maintained greater firmness and a higher level of soluble solid content (SSC) after 12 weeks of post-harvest storage at 0 °C. Comparative transcriptome analysis revealed these phenotypic changes to be associated with increased expression of APETALA2 (AP2) homologs MdAP2_1b/2a (miR172 target genes) and an Ethylene Response Factor (ERF) MdERF4, along with reduced expression of ethylene biosynthesis genes MdACS1/ACO1. In a dual luciferase reporter assay, MdAP2_1a/2a activated the MdERF4 promotor in tobacco leaves. These findings, together with existing knowledge that MdERF4 inhibits MdACS1/ACO1 expression, suggest a novel genetic regulatory network involving miR172, MdAP2 and MdERF4 in controlling apple fruit ripening.

分类号：

  • 相关文献

[1]PpIAA1 and PpERF4 form a positive feedback loop to regulate peach fruit ripening by integrating auxin and ethylene signals. Xiaobei Wang,Lei Pan,Yan Wang,Junren Meng,Li Deng,Liang Niu,Hui Liu,Yifeng Ding,Jia Long Yao,Nicolaas Jacobus Nieuwenhuizen,Charles Ampomah-Dwamena,Zhenhua Lu,Guochao Cui,Zhiqiang Wang,Wenfang Zeng. 2021

[2]Diverse Functions of IAA-Leucine Resistant PpILR1 Provide a Genic Basis for Auxin-Ethylene Crosstalk During Peach Fruit Ripening. Xiaobei Wang,Junren Meng,Li Deng,Yan Wang,Hui Liu,Jia-Long Yao,Nicolaas Jacobus Nieuwenhuizen,Zhiqiang Wang,Wenfang Zeng. 2021

[3]RNA-protein interactions reveals the pivotal role of lncRNA1840 in tomato fruit maturation. Zhu, Guoning,Li, Ran,Zhang, Lingling,Ma, Liqun,Li, Jinyan,Chen, Jieyin,Deng, Zhiping,Yan, Shijie,Li, Tao,Ren, Huazhong,Cui, Kaicheng,Qu, Guiqin,Zhu, Benzhong,Fu, Daqi,Luo, Yunbo,Zhu, Hongliang. 2024

[4]Expression Analysis Of Nac Genes D.uring The Growth And R ipening Of Apples. Dong, Wenxuan,Li, Tong,Wang, Aide,Zhang, Qijing,Zhang, Lijie,Zhang, Qijing. 2018

[5]ECTOPIC EXPRESSION OF SUBUNIT A OF VACUOLAR H+-ATPASE FROM APPLE ENHANCES SALT TOLERANCE IN TOBACCO PLANTS. Dong, Q. L.,Liu, D. D.,Wang, Q. J.,Fang, M. J.,Hao, Y. J.,Yao, Y. X.,Dong, Q. L.,Liu, D. D..

[6]Bioinformatic identification and expression analysis of the Malus domestica DREB2 transcription factors in different tissues and abiotic stress. Li, Huifeng,Sun, Xiaoli,Jia, Houzhen,Ran, Kun,Zhao, Qiang.

[7]Cryopreservation of apple dormant buds and shoot tips. Wu, YJ,Zhao, YH,Engelmann, F,Zhou, MD,Zhang, DM,Chen, SY. 2001

[8]Bioinformatic Identification And Expression Analysis O.f The Malus Domestica D reb2 Transcription Factors In Different Tissues And Abiotic Stress. Li, HF, Zhao, Q, Sun, XL, Jia, HZ, Ran, K. 2017

[9]Multielement authentication of apples from the cold highlands in southwest China. Zhang, Jianyi,Nie, Jiyun,Zhang, Liangbin,Xu, Guofeng,Zheng, Haidong,Shen, Youming,Kuang, Lixue,Gao, Xiaoqin,Zhang, Hui. 2022

[10]Metabolite changes of apple Penicillium expansum infection based on a UPLC-Q-TOF metabonomics approach. Youming Shen,Mingyu Liu,Jiyun Nie,Ning Ma,Guofeng Xu,Jianyi Zhang,Yinping Li,Haifei Li,Lixue Kuang,Zhiyuan Li. 2021

[11]Reactive Oxygen Species Metabolism And P.henylpropanoid Pathway Involved In D isease Resistance Against Penicillium Expansum In Apple Fruit Induced By E-Poly-L-Lysine. Ge, YH, Wei, ML, Li, CY, Chen, YR, Lv, JY, Meng, K, Wang, WH, Li, JR. 2018

[12]Genetic identification, pathogenicity and patulin production of Penicillium species from apple blue mold in China. Youming Shen,Wenyu Ma,Ning Ma,Mengyao Li,Jianyi Zhang,Guofeng Xu. 2024

[13]Over-expression of GhDWF4 gene improved tomato fruit quality and accelerated fruit ripening. Ye Shu-e,Li Fang,Li Xian-bi,Zhai Yun-lan,Hu Ming-yu,Wei Ting,Deng Sha-sha,Pei Yan,Luo Ming,Hong Qi-bin. 2015

[14]Functional analysis of sucrose synthase PavSS1 regulating sweet cherry fruit ripening [蔗糖合成酶PavSS1调控甜樱桃果实成熟的功能分析]. Qi X.,Liu C.,Song L.,Li M.. 2021

[15]Genome-wide transcriptome analysis uncovers gene networks regulating fruit quality and volatile compounds in mango cultivar 'Tainong' during postharvest. Li L.,Yi P.,Sun J.,Tang J.,Liu G.,Bi J.,Teng J.,Hu M.,Yuan F.,He X.,Sheng J.,Xin M.,Li Z.,Li C.,Tang Y.,Ling D.. 2023

[16]Transcriptome Profiling of Transposon-Derived Long Non-coding RNAs Response to Hormone in Strawberry Fruit Development. Chen, Xi,Wang, Chengdong,He, Bing,Wan, Zifan,Zhao, Yukun,Hu, Fengqin,Lv, Yuanda. 2022

[17]Characterization and Identification of a Ripening-Related Gene AaPG18 in Actinidia arguta. Yukuo Li,Hailei Huang,Muhammad Abid,Hong Gu,Jinbao Fang,Zhongping Cheng,Xiujuan Qi. 2022

[18]Sucrose non-fermenting1-related protein kinase VcSnRK2.3 promotes anthocyanin biosynthesis in association with VcMYB1 in blueberry. Wang, Xuan,Tang, Qi,Chi, Fumei,Liu, Hongdi,Zhang, Hongjun,Song, Yang. 2023

[19]Time-Series Transcriptome of Cucumis melo Reveals Extensive Transcriptomic Differences with Different Maturity. Liu F.,Shao X.,Fan Y.,Jia B.,He W.,Wang Y.,Wang F.,Wang C.. 2024

[20]Genome-wide analysis of AP2/ERF family in Akebia trifoliata and characterization of an AtrERF001 gene regulate fruit ripening. Juan Niu,Ying Li,Zhimin Sun,Ying Lin,Chao Zhang,Bo Yang,Shuang Tian,Mingbao Luan. 2025

作者其他论文 更多>>