数字农科院2.0

Genome-wide association study reveals the genetic basis of vitamin C content in rapeseed (Brassica napus L.) seedlings

文献类型： 外文期刊

作者： Chao Wang;Lieqiong Kuang;Ze Tian;Xinfa Wang;Hanzhong Wang;Xiaoling Dun

作者机构：

关键词： candidate genes;germplasm resource;GWAS;rapeseed seedlings;vitamin C

期刊名称： Frontiers in Plant Science

ISSN： 1664-462X

年卷期： 2025 年 16 卷

页码：

收录情况： SCIE（2025版）

摘要： Rapeseed (Brassica napus L.) is a versatile crop, with its seedlings and flowering stalks can be utilized as vegetables, which are rich in vitamin C (Vc) and other essential nutrients, including selenium, calcium, zinc, and various amino acids. Despite the well-documented health benefits of Vc as a critical antioxidant nutrient, the genetic mechanisms governing Vc accumulation in rapeseed remain poorly understood. In this study, we investigated the Vc content of 327 rapeseed accessions during the seedling stage across six environments in Hubei province over three consecutive years (2018-2020). The Vc content in these environments ranged from 62.82 to 161.25 mg/100g, demonstrating high genetic variation (7.96% to 9.43%) and heritability (86.11%). Genome-wide association studies (GWAS) identified 31 significant single nucleotide polymorphisms (SNPs) across various chromosomes, which explained 5.68% to 12.78% of the phenotypic variation, integrated into 16 quantitative trait loci (QTLs). Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses of the 2365 annotated genes near associated SNPs revealed significant involvement in diverse metabolic pathways including peroxisome, ascorbate, secondary metabolites, and terpenoid biosynthesis, as well as biological processes such as hydrogen peroxide/lactate metabolism and ROS biosynthesis, along with associations with specific cellular components and molecular functions. Furthermore, we identified six candidate genes that exhibit significant differences in expression between low and high Vc accessions, which are potentially involved in Vc biosynthesis but require further experimental validation. Additionally, we selected four superior germplasms (8S079, 8S200, 8S242, and 8S243) that demonstrate extreme Vc content, providing valuable germplasm resources for breeding. Collectively, these findings provide novel and comprehensive insights into the genetic and molecular mechanisms regulating Vc accumulation in rapeseed, thereby establishing a foundation for targeted genetic improvement of nutritional quality in vegetable rapeseed varieties.

分类号：

  • 相关文献

[1]Integrating GWAS and transcriptomics to identify candidate genes conferring heat tolerance in rice. Pingping Li ,Jing Jiang,Guogen Zhang,Siyu Miao,Jingbing Lu,Yukang Qian,Xiuqin Zhao,Wensheng Wang,Xianjin Qiu,Fan Zhang,and Jianlong Xu. 2023

[2]Genetic Analysis of Vitamin C Content in Rapeseed Seedlings by the Major Gene Plus Polygene Mixed Effect Model. Chao Wang,Tao Wang,Xinfa Wang,Hanzhong Wang,Xiaoling Dun. 2024

[3]Genome-Wide Association Study Reveals the Genetic Basis of Crude Fiber Components in Brassica napus L. Shoots at Stem Elongation Stage. Shi, Rui,Cao, Yu,Yang, Tinghai,Wang, Yaping,Xiang, Yanan,Chen, Feng,Zhang, Wei,Zhou, Xiaoying,Sun, Chengming,Fu, Sanxiong,Hu, Maolong,Zhang, Jiefu,Wang, Xiaodong. 2024

[4]Genome-Wide Linkage Analysis and Association Study Identifies Loci for Polydactyly in Chickens. Sun, Yanfa,Liu, Ranran,Zhao, Guiping,Zheng, Maiqing,Sun, Yan,Yu, Xiaoqiong,Li, Peng,Wen, Jie,Sun, Yanfa,Liu, Ranran,Zhao, Guiping,Zheng, Maiqing,Wen, Jie,Sun, Yanfa. 2014

[5]Identification of loci and genes for growth related traits from a genome-wide association study in a slow- x fast-growing broiler chicken cross. Liu, Ranran,Sun, Yanfa,Zhao, Guiping,Wang, Hongyang,Zheng, Maiqing,Li, Peng,Liu, Li,Wen, Jie,Sun, Yanfa,Liu, Ranran,Zhao, Guiping,Wang, Hongyang,Zheng, Maiqing,Wen, Jie.

[6]The identification of 14 new genes for meat quality traits in chicken using a genome-wide association study. Sun, Yanfa,Zhao, Guiping,Liu, Ranran,Zheng, Maiqing,Hu, Yaodong,Wu, Dan,Zhang, Lei,Li, Peng,Wen, Jie,Zhao, Guiping,Liu, Ranran,Zheng, Maiqing,Li, Peng,Wen, Jie,Sun, Yanfa,Wen, Jie. 2013

[7]High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.). Jingyang Tong,Cong Zhao,Mengjing Sun,Luping Fu,Jie Song,Dan Liu,Yelun Zhang,Jianmin Zheng,Zongjun Pu,Lianzheng Liu,Awais Rasheed,Ming Li,Xianchun Xia,Zhonghu He,Yuanfeng Hao. 2022

[8]A genome-wide association study of lateral root number for Asian cotton (Gossypium arboreum L.). Hu Daowu,He Shoupu,Sun Gaofei,Jia Yinhua,Geng Xiaoli,Wang Liru,Pan Zhaoe,Chen Baojun,Li Hongge,Zubair, Iqbal,Wang Xiaoyang,Zhao Zibo,Ge Yuting,Pang Baoyin,Du Xiongming. 2022

[9]Genome-wide Association Study for Starch Pasting Properties in Chinese Spring Wheat. Tian Y.,Sang W.,Liu P.,Liu J.,Xiang J.,Cui F.,Xu H.,Han X.,Nie Y.,Kong D.,Li W.,Mu P.. 2022

[10]Genome-Wide Association Study Discovered Favorable Single Nucleotide Poly Morphisms and Candidate Genes Associated with Ramie (Boehmeria Nivea L.) Colloidal Matters. Chen, Kunmei,Ou, Wenjing,Yu, Chunming,Zhu, Aiguo,Luan, Mingbao,Chen, Jikang,Gao, Gang,Wang, Xiaofei, I,Chen, Ping. 2023

[11]A Large-Scale Genomic Association Analysis Identifies the Candidate Genes Regulating Salt Tolerance in Cucumber (Cucumis sativus L.) Seedlings. Dongrang Liu,Shaoyun Dong,Han Miao,Xiaoping Liu,Caixia Li,Jianan Han,Shengping Zhang,Xingfang Gu. 2022

[12]Genome-wide association and RNA-seq analyses identify loci for pod orientation in rapeseed (Brassica napus). Yang Y.,Wang W.,Hu Q.,Raman H.,Liu J.. 2023

[13]Identification of Candidate Genes that Affect the Contents of 17 Amino Acids in the Rice Grain Using a Genome-Wide Haplotype Association Study. Xiaoqian Wang,Lihong Xie,Jiachuang Fang,Yunlong Pang,Jianlong Xu,Zhikang Li. 2023

[14]Decryption of superior allele and candidate genes for total lignin contents of rapeseed. Wenkai Yu,Yuting Yang,Liezhao Liu,Zhi Li,Cheng Zhu,Qiong Hu,Jia Liu. 2024

[15]Identification of novel candidate loci and genes for seed vigor-related traits in upland cotton (Gossypium hirsutum L.) via GWAS. Libei Li,Yu Hu,Yongbo Wang,Shuqi Zhao,Yijin You,Ruijie Liu,Jiayi Wang,Mengyuan Yan,Fengli Zhao,Juan Huang,Shuxun Yu,Zhen Feng. 2023

[16]Genetic Diversity, Selection Signatures, and Genome-Wide Association Study Identify Candidate Genes Related to Litter Size in Hu Sheep. Jingjing Bao,Jinke Xiong,Jupeng Huang,Peifu Yang,Mingyu Shang,Li Zhang. 2024

[17]Genetic dissection of crown root traits and their relationships with aboveground agronomic traits in maize. Xiao qian SHA,Hong hui GUAN,Yu qian ZHOU,Er hu SU,Jian GUO,Yong xiang LI,Deng feng ZHANG,Xu yang LIU,Guan hua HE,Yu LI,Tian yu WANG,Hua wen ZOU,Chun hui LI. 2023

[18]Genome wide association and haplotype analyses for the crease depth trait in bread wheat (Triticum aestivum L.). Chengxiang Song,Kaidi Xie,Xin Hu,Zhihua Zhou,Ankui Liu,Yuwei Zhang,Jiale Du,Jizeng Jia,Lifeng Gao,Hailiang Mao. 2023

[19]Candidate Genes and Favorable Haplotypes Associated with Iron Toxicity Tolerance in Rice. Siyu Miao,Jingbing Lu,Guogen Zhang,Jing Jiang,Pingping Li,Yukang Qian,Wensheng Wang,Jianlong Xu,Fan Zhang,Xiuqin Zhao. 2024

[20]Genome-Wide Association Study Reveals Marker–Trait Associations for Heat-Stress Tolerance in Sweet Corn. Quannv Yang,Zifeng Guo,Jianan Zhang,Yunbo Wang,Yunbi Xu,Hai Nian. 2024

作者其他论文 更多>>