数字农科院2.0

m6A modification of plant virus enables host recognition by NMD factors in plants

文献类型： 外文期刊

作者： He, Hao;Ge, Linhao;Chen, Yalin;Zhao, Siwen;Li, Zhaolei;Zhou, Xueping;Li, Fangfang

作者机构：

关键词： m(6)A;Pepino mosaic virus;plant defense;m(6)A readers;NMD factors

期刊名称： SCIENCE CHINA-LIFE SCIENCES

ISSN： 1674-7305

年卷期： 2023 年

页码：

收录情况： SCIE（2023版） ; ; CSCD（2023-2024年度） ; ; 科技核心（2023版）

摘要： N-6-methyladenosine (m(6)A) is the most abundant eukaryotic mRNA modification and is involved in various biological processes. Increasing evidence has implicated that m(6)A modification is an important anti-viral defense mechanism in mammals and plants, but it is largely unknown how m(6)A regulates viral infection in plants. Here we report the dynamic changes and functional anatomy of m(6)A in Nicotiana benthamiana and Solanum lycopersicum during Pepino mosaic virus (PepMV) infection. m(6)A modification in the PepMV RNA genome is conserved in these two species. Overexpression of the m6A writers, mRNA adenosine methylase A (MTA), and HAKAI inhibit the PepMV RNA accumulation accompanied by increased viral m6A modifications, whereas deficiency of these writers decreases the viral RNA m6A levels but enhances virus infection. Further study reveals that the cytoplasmic YTH-domain family protein NbECT2A/2B/2C as m(6)A readers are involved in anti-viral immunity. Protein-protein interactions indicate that NbECT2A/2B/2C interact with nonsense-mediated mRNA decay (NMD)-related proteins, including NbUPF3 and NbSMG7, but not with NbUPF1. m(6)A modification-mediated restriction to PepMV infection is dependent on NMD-related factors. These findings provide new insights into the functionality of m6A anti-viral activity and reveal a distinct immune response that NMD factors recognize the m(6)A readers-viral m6A RNA complex for viral RNA degradation to limit virus infection in plants.

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