数字农科院2.0

Molybdenum cofactor biosynthesis gene ZmCNX6 regulates vivipary and drought tolerance in maize

文献类型： 外文期刊

作者： Chen, Yuxin;Wang, Yiru;Sun, Minghao;Li, Jian;Qin, Yang;Huang, Quansheng;Zheng, Jun

作者机构：

关键词： Maize;Vivipary;Molybdenum cofactor (MoCo);Abscisic acid (ABA);Drought

期刊名称： CROP JOURNAL

ISSN： 2095-5421

年卷期： 2025 年 13 卷 5 期

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： Pre-harvest sprouting (PHS) or vivipary is a major problem affecting cereal quality and grain quantity and is primarily linked to the dysregulation of abscisic acid (ABA) biosynthesis in plants. Therefore, elucidating the molecular mechanisms governing seed dormancy is crucial for developing strategies to improve crop productivity. In this study, we identified a novel viviparous maize mutant, viviparous-like 5 (vp-like5), which exhibits precocious germination in developing seeds. Through map-based cloning, we discovered that ZmCNX6, which encodes a small subunit of molybdopterin synthase essential for molybdenum cofactor (MoCo) biosynthesis, is the causal gene responsible for the vp-like5 phenotype. Biochemical assays have demonstrated significantly reduced activities of MoCo-dependent enzymes, including aldehyde oxidase (AO), xanthine dehydrogenase (XDH), and nitrate reductase (NR), in vp-like5. AO is essential for the ABA biosynthesis, and the observed ABA deficiency in vp-like5 likely drives the viviparous phenotype. Expression analysis showed that ZmCNX6 was stably expressed during seed development, indicating its significant role in seed development. Furthermore, overexpression of ZmCNX6 not only enhanced the activities of MoCo-dependent enzymes but also improved drought tolerance in maize. Collectively, our study revealed ZmCNX6 as a multifunctional hub coordinating MoCo metabolism, ABA-dependent dormancy regulation, and abiotic stress responses, offering a potential target for simultaneously mitigating vivipary and improving drought resistance in maize. (c) 2025 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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