数字农科院2.0

Identification and analysis of miRNA-mRNA regulatory networks of ZM-4 (Malus zumi) in response to salt stress

文献类型： 外文期刊

作者： Liu, Zhao;Gao, Yuan;Guo, Hanxin;Shang, Wei;Wang, Kun;Sun, Simiao;Wang, Lin;Tian, Wen;Li, Zichen;Sun, Yanming;Li, Lianwen;Niu, Jianxin;Wang, Dajiang

作者机构：

关键词： Malus zumi;Salt stress;miRNA;mRNA;Regulatory network

期刊名称： PLANT PHYSIOLOGY AND BIOCHEMISTRY

ISSN： 0981-9428

年卷期： 2025 年 230 卷

页码：

收录情况： SCIE（2025版）

摘要： Malus zumi is an excellent saline-alkali tolerant apple rootstock resource and a valuable economic tree for greening due to its ornamental value. It is of great potential significance in the production of apples in salinealkali areas. miRNA is a class of endogenous non-coding small RNA, which plays an important role in plant resistance to salt stress. However, the miRNA-mediated regulatory mechanisms underlying salt tolerance in Malus zumi remain unclear. In this study, the salt-tolerant apple resource Malus zumi ZM-4 and the salt-sensitive rootstock M9T337 were used as experimental materials. High-throughput sequencing was conducted on the leaves and roots of these plants treated with 75 mmol/L NaCl for 0 h and 24 h. The results showed that a total of 3280 miRNAs were identified, with 274 and 323 miRNAs were specifically expressed in ZM-4 and M9T337, respectively. Under salt stress, 299 and 654 differentially expressed miRNAs were detected in leaves and roots, targeting 994 and 1813 mRNAs, respectively. Furthermore, five (mdm-miR395j-TPS13; miR1507-y-RGA1; miR3699-z-SPL13A; novel-m0411-5p-MKK4; novel-m0641-3p/novel-m0642-3p-ALMT4) and three (miR168-yPKS5; novel-m0474-3p-CNGC10; mdm-miR156a similar to mdm-miR156o-SPL2/SPL6/SPL12) potential miRNA-mRNA regulatory modules were identified to response to salt stress in the leaves and roots, respectively. The qRTPCR validation results were consistent with the RNA-seq results. A dual-luciferase assay further confirmed that mdm-miR156b negatively regulated the expression of MdSPL6. This study revealed the key regulatory network of miRNAs in the leaves and roots of ZM-4 to respond to salt stress, and the results laid the foundation for the post-transcriptional molecular regulatory mechanism of salt tolerance in ZM-4.

分类号：

  • 相关文献

[1]Whole transcriptome sequencing-based identification and functional prediction of salt-tolerant-related circular RNAs in ZM-4 (Malus zumi). Zhao Liu,Xiang Lu,Hanxin Guo,Wei Shang,Yuan Gao,Simiao Sun,Kun Wang,Wen Tian,Lin Wang,Zichen Li,Lianwen Li,Jianxin Niu,Dajiang Wang. 2025

[2]Genome-wide transcriptome analysis of mRNAs and microRNAs in Dorset and Small Tail Han sheep to explore the regulation of fecundity. Miao, Xiangyang,Qingmiao Luo Xiaoyu Qin. 2015

[3]Genome-wide analysis reveals the differential regulations of mRNAs and miRNAs in Dorset and Small Tail Han sheep muscles. Miao, Xiangyang,Luo, Qingmiao,Qin, Xiaoyu.

[4]Comparison of miRNA and mRNA Expression in Sika Deer Testes With Age. Jia B.,Zhang L.,Ma F.,Wang X.,Li J.,Diao N.,Leng X.,Shi K.,Zeng F.,Zong Y.,Liu F.,Gong Q.,Cai R.,Yang F.,Du R.,Chang Z.. 2022

[5]Transcriptome analysis reveals a lncRNA-miRNA-mRNA regulatory network in OsRpp30-mediated disease resistance in rice. Minghua Li,Wei Li,Meixia Zhao,Zhiqiang Li,Guo Liang Wang,Wende Liu,Chun Liang. 2023

[6]Comprehensive analysis of differentially expressed mRNAs, circRNAs, and miRNAs and their ceRNA network in the testis of cattle–yak, yak, and cattle. Mengli Cao,Lin Xiong,Xingdong Wang,Shaoke Guo,Liyan Hu,Yandong Kang,Xiaoyu Wu,Pengjia Bao,Min Chu,Chunnian Liang,Jie Pei,Xian Guo. 2024

[7]Integrated analysis of miRNA and mRNA expression profiles in the bursa of Fabricius of specific pathogen-free chickens infected with avian reticuloendotheliosis virus strain SNV. Yubo Zhao,Qing Zhang,Meng Wang,Bingrong Wu,Saisai Zhao,Xinhui Wei,Youxiang Diao,Yi Tang,Jingdong Hu. 2025

[8]Improvement of plant tolerance to salt stress by GhRL192 mRNA through long-distance movement. Xinjie Zhang,Wenqian Liu,Tianen Zhang,Jinzhu Li,Jie He,Yuanyuan Liu,Lu Yang,Yuan Wang,Jun Peng,Zhanshuai Li. 2025

[9]MicroRNA and target gene responses to salt stress in grafted cucumber seedlings. Li, Yansu,Li, Chaohan,Bai, Longqiang,He, Chaoxing,Yu, Xianchang,Li, Chaohan. 2016

[10]Genome-wide characterization of salt-responsive mirnas, circrnas and associated cerna networks in tomatoes. Zhongyu Wang,Ning Li,Qinghui Yu,Huan Wang. 2021

[11]肉鸡限制饲养研究新进展. 李敏,王焕杰,陈小鸽,张雪洁,商艳红,陈宇,黄艳群,路兵龙,陈文. 2018

[12]日粮赖氨酸的不同添加水平对肉仔鸡肝脏和肌肉中IGF-1mRNA丰度影响的研究. 陈志敏,蔡辉益,刘国华,常文环,张姝,于会民. 2004

[13]不同剂量胰岛素、胰高血糖素和甲状腺素处理对奶牛乳腺上皮细胞FcRn mRNA表达的影响. 胡小丽,张春林,王加启,赵圣国,章玉涛,卜登攀,周凌云. 2011

[14]PGC-1β基因在鸡骨骼肌中的表达差异研究. 章明,单艳菊,束婧婷,徐文娟,李慧芳. 2015

[15]通城猪ADSL基因在两个时期不同组织中的mRNA表达分析. 刘基伟,李奎,徐日福,任红艳,肖邦,白立景. 2009

[16]以组织锌、金属硫蛋白及其基因表达指标评价肉仔鸡对锌源的相对生物学利用率. 曹家银,罗绪刚,S.R.Davis,P.R.Henry,R.J.Cousins,R.D.Miles,C.B.Ammerman. 2003

[17]RNA干扰及其在动物传染病方面的研究概况. 杨亮宇,孙永科,杨玉艾,王凯,王玉娥,孔令富. 2012

[18]能量水平对文昌鸡开产前后NPY基因mRNA表达以及开产性状的影响. 朱文奇,李慧芳,宋卫涛,杜德英,吴蓉蓉,徐文娟,肖小珺,陈宽维. 2011

[19]用改进的Mg~(++)沉淀多聚核糖体法分离胞质mRNA. 张西平,黄磊,张楚瑜,王鄂生,夏伏建. 1998

[20]实时荧光定量PCR对犬乳腺肿瘤组织VEGF-C基因的定量检测研究. 邱昌伟,王亨,陈双燕,林德贵,宋筱瑜,王金秋. 2007

作者其他论文 更多>>